What makes the filtration process superior to standard 0.22 µm filtered media?

The FluxMPS™ Quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) targets the 0.04 µm particulate range - roughly 5× cleaner than conventional 0.22 µm media by particulate count - and provides a 40 nm mycoplasma-scale barrier (USP less than 63greater than -equivalent assurance).

What endotoxin level should I expect, and how is it verified?

The specification is less than 0.05 EU/mL, verified by bacterial endotoxin testing per USP less than 85greater than (BET). The measured value for each lot is reported on the Certificate of Analysis.

Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. A CoA accompanies every lot of DCP-IMDM-B1X with measured QC values such as appearance, pH, osmolality, sterility, and endotoxin. Request one at less than a href="mailto:support@diagnocine.com"greater than support@diagnocine.comless than /agreater than .

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FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM) High Glucose w/o Sodium Bicarbonate: 1X Liquid

Q: Is DCP-IMDM-B1X suitable for organ-on-a-chip (OoC) applications?

Yes. DCP-IMDM-B1X is nano-filtered to a 0.04 µm final polish and formulated with Ultrapure Type 1 water specifically to minimize subvisible particulates that clog microchannels and corrupt biosensor signals, making it well suited to OoC, MPS, and microfluidic perfusion.

Product#: DCP-IMDM-B1X

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DCP-IMDM-B1X

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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM) High Glucose w/o Sodium Bicarbonate: 1X Liquid

An MPS-grade, high-glucose Iscove's Modified Dulbecco's Medium re-engineered for microphysiological systems and microfluidic channels. Quadruple-stage ultra-filtration (0.1 μm ×2 + 0.04 μm ×2) delivers media approximately 5× cleaner than conventional 0.22 μm formulations, with an endotoxin specification below 0.05 EU/mL and Ultrapure Type 1 water.

Nano-filtered, microchannel-safe formulation engineered for microphysiological systems (MPS) and organ-on-a-chip (OoC) platforms
FluxMPS™ Quadruple-stage filtration architecture (0.1 μm ×2 + 0.04 μm ×2) for ultra-low particulate, sub-mycoplasma polishing
Endotoxin specification < 0.05 EU/mL by USP <85> BET, supporting low-background live-cell imaging and biosensing
Iscove's Modified Dulbecco's Medium (high-glucose); without sodium bicarbonate
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for ultra-clean microfluidic perfusion
ISO Class 5 (Class 100) aseptic fill & finish under an ISO 13485:2016 quality management system
pH, glucose concentration, salts, HEPES, and nutrient composition available on request

DCP-IMDM-B1X| Size: 500 mL and 1000 mL| UNSPSC 41122100| Cell Culture Media

Iscove's Modified Dulbecco's Medium (IMDM) High Glucose w/o Sodium Bicarbonate: 1X Liquid, sterile-filtered

Glucose4500.000 mg/L
L-Glutamine584.000 mg/L
Sodium Pyruvate110.000 mg/L
pH (USP <791>)7.4
Osmolality (USP <785>)210~250 mOsm/kg H₂O
Endotoxin< 0.05 EU/mL
FiltrationQuadruple-stage (0.1 μm ×2 + 0.04 μm ×2)
Storage2~8 °C, protected from light
Shelf Life12 months
ShippingCold-pack

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard 0.22 μm media fails

Conventional 0.22 μm-filtered media can carry subvisible particulates and mycoplasma-scale contaminants (the smallest mycoplasma are ~0.2 μm) that accumulate in microfluidic channels, corrupt biosensor signals, and shorten perfusion runs. FluxMPS™ IMDM, High Glucose w/o Sodium Bicarbonate is re-engineered for these microphysiological systems.^[1,2]

filter_alt

Microchannel-safe purity

A 0.04 μm final-polish filter targets subvisible particulates per USP <788> Method 2, preventing the microchannel accumulation and clogging that degrade organ-on-a-chip experiments.

target

Total metabolic control

A defined, user-tunable carbon-source and nutrient backbone supports Warburg-effect and metabolic-flux studies where standard serum-rich media confound the readout.

water_drop

Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), reducing ionic and organic background consistent with USP <85> endotoxin control.

visibility

Low background for imaging

Ultra-clean, low-particulate fluid minimizes optical scatter and autofluorescence for confocal microscopy, biosensors, and TEER measurements.

science

Rich, stable nutrient profile

IMDM's enriched amino-acid and vitamin profile, selenium, and sodium pyruvate are released on a micro-batch basis for lot-to-lot consistency.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrient composition can be adjusted on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

The FluxMPS™ purity architecture is built around a four-stage membrane train (0.1 μm ×2 + 0.04 μm ×2), positioning DCP-IMDM-B1X as a ready-to-use, ultra-clean medium for microfluidic and organ-on-a-chip work at a purity level conventional 0.22 μm media cannot reach.^[2,3]

1

0.1 μmPre-filtration I

Large particulate and aggregate removal that extends downstream filter life and protects the polishing stages.
2

0.04 μmPre-filtration II

Fine-particulate reduction with bacteria and mycoplasma-scale retention ahead of sterile filtration.
3

0.1 μmSterile-filtration I

Second-pass redundancy for robust, validated sterility assurance.
4

0.04 μmSterile-filtration II - Final Polish

Ultimate ultra-low-particulate polish, filled under an ISO Class 5 (Class 100) aseptic environment.

Performance vs. conventional media

By targeting the 0.04 μm particulate range, the FluxMPS™ train delivers approximately 5× cleaner fluid than conventional 0.22 μm-filtered media by particulate count — protecting microchannels, valves, and optical sensors.

5×

cleaner than 0.22 μm media (particulate count)

0.04

μm final-polish membrane

Sterility & mycoplasma assurance. No bacterial or fungal growth after 14 days of incubation per USP <71> specification. Filtration to 40 nm provides a USP <63>-equivalent mycoplasma barrier (the smallest mycoplasma are ~0.2 μm).

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM) High Glucose w/o Sodium Bicarbonate: 1X Liquid (DCP-IMDM-B1X) w/o sodium bicarbonate ? Quadruple-stage filtration (0.1 μm ×2 + 0.04 μm ×2) ultra-filtered IMDM cell culture media for organ-on-a-chip, microphysiological system (MPS) and microfluidic applications | Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration architecture — two 0.1 μm pre-filtration passes followed by two 0.04 μm sterile-polish passes for ultra-low-particulate, microchannel-safe media.
© Diagnocine® — DCP-IMDM-B1X

Applications

Built for microphysiological & organ-on-a-chip research

IMDM, High Glucose w/o Sodium Bicarbonate supports rapidly proliferating, high-density cultures — from erythroid progenitors, macrophages, and B/T lymphocytes to hybridomas — and is well suited to perfused MPS, metabolic, and imaging applications.^[1,4]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors and robotic perfusion platforms, an optional 10 nm (0.01 μm) ultra-filtered grade is available to push particulate exclusion beyond the standard 0.04 μm polish.

Total particulate exclusion for narrow microchannels and high-resolution optics
Valve & sensor protection across long unattended runs
Extended perfusion stability for multi-week organ-on-a-chip experiments

Inquiry Required: the optional 0.01 μm (10 nm) grade is made to order — contact support@diagnocine.com to request it.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low-particulate fluid for perfused chips and barrier models.

OoCToCBoCLoCMPS

Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon-source backbone for tumor-metabolism studies.

MCF-7MDA-MB-231HeLaA549

Stem Cell Biology

iPSC-Derived Models

Consistent nutrient profile for differentiated iPSC cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cells

Low-background medium for vessel-on-chip and primary cells.

HUVECsHAECsPrimary hepatocytes

Metabolomics

Metabolic Flux Analysis

Clean baseline for tracer and respirometry workflows.

¹³C tracingSeahorse XFNMR metabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Low autofluorescence and scatter for long-term imaging.

ConfocalBiosensorsTEER

Technical Specifications

Technical specifications

Representative specifications for DCP-IMDM-B1X. Values are confirmed per lot on the Certificate of Analysis.

Physical & Chemical Parameters

Parameter	Specification
Formulation	Iscove's Modified Dulbecco's Medium (IMDM), 1X liquid (w/o sodium bicarbonate)
Appearance	Orange-Red-colored, clear solution.
pH USP <791>	7.4 at 1X
Osmolality USP <785>	210~250 mOsm/kg H₂O
Glucose	4500.000 mg/L
L-Glutamine	584.000 mg/L
Sodium Pyruvate	110.000 mg/L
Phenol Red	15.000 mg/L

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	Filtered in a sterile environment. No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
Mycoplasma	40 nm filtration barrier; USP <63>-equivalent assurance
Particulate ≥10 μm USP <788>	Meets USP <788> Method 2 limits
Particulate ≥25 μm USP <788>	Meets USP <788> Method 2 limits
Water purity	Ultrapure Type 1 (18.2 MΩ·cm)
Manufacturing std. ISO	ISO 13485:2016; 21 CFR Part 820 (cGMP) aligned
Fill environment	ISO Class 5 (Class 100) aseptic

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2~8 °C, away from bright light
Freeze-thaw	Avoid repeated freeze-thaw
Shelf life	12 months from manufacture
Shipping condition	Cold-pack
CO₂ requirement	Reduced/independent CO₂ (no bicarbonate buffer) verify for your system

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Cell-culture grade, traceable lots
Traceability	Full lot traceability with CoA
Manufacturing QMS	ISO 13485:2016 certified facility
Regulatory alignment	21 CFR Part 820 (cGMP) aligned; CE-approved supplier facility
Production method	Micro-batch, ISO Class 5 fill & finish
Intended use	For Research Use Only (RUO)

Formulation

Full composition (mg/L)

Complete defined formulation of DCP-IMDM-B1X, released on a per-lot basis. All 40 components and their mg/L values are reproduced from the validated formulation.

INORGANIC SALTS
Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	219.000
Magnesium sulphate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	330.000
Potassium nitrate	7757-79-1	0.076
Sodium chloride	7647-14-5	4505.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	109.000
Sodium selenite	10102-18-8	0.0173

AMINO ACIDS
Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	30.000
L-Alanine	56-41-7	25.000
L-Arginine hydrochloride	1119-34-2	84.000
L-Asparagine	70-47-3	25.000
L-Aspartic acid	56-84-8	30.000
L-Cystine dihydrochloride	30925-07-6	91.240
L-Glutamic acid	56-86-0	75.000
L-Glutamine	56-85-9	584.000
L-Histidine hydrochloride monohydrate	5934-29-2	42.000
L-Isoleucine	73-32-5	104.800
L-Leucine	61-90-5	104.800
L-Lysine hydrochloride	657-27-2	146.200
L-Methionine	63-68-3	30.000
L-Phenylalanine	63-91-2	66.000
L-Proline	147-85-3	40.000
L-Serine	56-45-1	42.000
L-Threonine	72-19-5	95.200
L-Tryptophan	73-22-3	16.000
L-Tyrosine disodium salt	69847-45-6	104.200
L-Valine	72-18-4	93.600

VITAMINS / OTHERS
Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	4.000
D-Biotin	58-85-5	0.013
D-Ca-Pantothenate	137-08-6	4.000
Folic acid	59-30-3	4.000
Nicotinamide	98-92-0	4.000
Pyridoxal hydrochloride	65-22-5	4.000
Riboflavin	83-88-5	0.400
Thiamine hydrochloride	67-03-8	4.000
Vitamin B12	68-19-9	0.013
i-Inositol	87-89-8	7.200
OTHERS
D-Glucose	50-99-7	4500.000
Phenol red sodium salt	34487-61-1	15.000
Sodium pyruvate	113-24-6	110.000

Need a custom formulation- pH, glucose, salts, HEPES, and nutrient composition can be adjusted — contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

All final packaging, quality assurance, and testing for DCP-IMDM-B1X are completed at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Manufactured under an ISO 13485:2016 quality management system at CE-approved supplier facilities, 21 CFR Part 820 (cGMP) aligned.

water_drop

Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm Ultrapure Type 1 water for ultra-low ionic and organic background.

biotech

ISO Class 5 Fill & Finish

Final 0.04 μm polish and aseptic fill performed in an ISO Class 5 (Class 100) environment.

assignment

Micro-Batch Precision

Micro-batch production with per-lot release testing for lot-to-lot consistency and traceability.

Endotoxin USP <85> BET

Bacterial endotoxin testing to a < 0.05 EU/mL specification.

Particulate USP <788> Method 2

Subvisible particulate matter controlled to USP <788> Method 2 limits.

Osmolality USP <785>

Osmolality verified per lot within the specified range.

Documentation / CoA

A Certificate of Analysis accompanies every lot with measured QC values.

A Certificate of Analysis (CoA) is available for every lot — request one at support@diagnocine.com.

Product Comparison

How DCP-IMDM-B1X compares

DCP-IMDM-B1X versus conventional 0.22 μm-filtered IMDM and a standard 0.22 μm-filtered DMEM-class alternative.

Parameter	DCP-IMDM-B1X (FluxMPS™)	Conventional IMDM (0.22 μm)	Standard DMEM-class (0.22 μm)
Formulation enrichment	Iscove-enriched amino acids/vitamins + selenium, potassium nitrate	IMDM base	DMEM base
Final filtration pore size	0.04 μm (40 nm)	0.22 μm	0.22 μm
Number of filtration stages	4 (0.1 μm ×2 + 0.04 μm ×2)	1	1
Mycoplasma barrier filtration	check_circle	cancel	cancel
Endotoxin specification	< 0.05 EU/mL	Typically < 1 EU/mL	Not always specified
USP <788> particulate compliance	check_circle	cancel	cancel
Water quality	Ultrapure Type 1 (18.2 MΩ·cm)	Type 1/2	Type 1/2
Manufacturing QMS	ISO 13485:2016	Varies	Varies
Microfluidic channel compatibility	check_circle	Limited	Limited
Custom formulation	check_circle	Limited	Limited

FAQ

Frequently asked questions

Common questions about DCP-IMDM-B1X for microphysiological and standard cell-culture workflows.

Yes. DCP-IMDM-B1X is nano-filtered to a 0.04 μm final polish and formulated with Ultrapure Type 1 water specifically to minimize subvisible particulates that clog microchannels and corrupt biosensor signals, making it well suited to OoC, MPS, and microfluidic perfusion.

The FluxMPS™ Quadruple-stage train (0.1 μm ×2 + 0.04 μm ×2) targets the 0.04 μm particulate range ? roughly 5× cleaner than conventional 0.22 μm media by particulate count ? and provides a 40 nm mycoplasma-scale barrier (USP <63>-equivalent assurance).

In DCP-IMDM-B1X, sodium bicarbonate is omitted to give you control over your culture conditions. To adapt it, add your own bicarbonate or an alternative buffer matched to your gas environment before use. Review the literature for the requirements of your specific cell line.

This formulation omits sodium bicarbonate, so the standard bicarbonate/CO₂ buffering is not provided; choose a buffer and gas environment appropriate to your system.

Yes. IMDM contains no proteins, lipids, or growth factors, so it is typically supplemented with serum or serum-free additives. It also serves as a base for some serum-free formulations.

The specification is < 0.05 EU/mL, verified by bacterial endotoxin testing per USP <85> (BET). The measured value for each lot is reported on the Certificate of Analysis.

Yes. A CoA accompanies every lot of DCP-IMDM-B1X with measured QC values such as appearance, pH, osmolality, sterility, and endotoxin. Request one at support@diagnocine.com.

Scientific References

Supporting literature

Peer-reviewed literature supporting the use of enriched, ultra-filtered media in microphysiological and metabolic research.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772.doi:10.1038/nbt.2989
Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell. 2018;22(3):310-324.doi:10.1016/j.stem.2018.02.011
Halldorsson S, et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218-231.doi:10.1016/j.bios.2014.07.029
Iscove NN, Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978;147(3):923-933.doi:10.1084/jem.147.3.923
Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029-1033.doi:10.1126/science.1160809
Nikolaev M, et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020;585(7826):574-578.doi:10.1038/s41586-020-2724-8
Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75-90.doi:10.1023/A:1022913015916
Booth R, Kim H. Characterization of a microfluidic in vitro model of the blood-brain barrier. Lab Chip. 2012;12(10):1784-1792.doi:10.1039/c2lc40094d
Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442(7101):368-373.doi:10.1038/nature05058
Low LA, Mummery C, Berridge BR, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361.doi:10.1038/s41573-020-0079-3