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FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-IMDMH-BR1X
$44.00
DCP-IMDMH-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

A Microfluidics Suitable, high-glucose Iscove's Modified Dulbecco's Medium re-engineered for microphysiological systems and microfluidic channels. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Nano-filtered, microchannel-safe IMDM formulation engineered for microphysiological systems (MPS) and organ-on-a-chip (OoC) platforms
  • FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) — two dedicated prefilter/final-filter pairs for ultra-low particulate, sub-mycoplasma polishing
  • Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85> BET), controlled per manufacturing batch
  • Iscove's Modified Dulbecco's Medium, high glucose (4500 mg/L), HEPES-buffered (25 mM); formulated without sodium bicarbonate and without phenol red
  • Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon control in microfluidic perfusion
  • ISO Class 5 (Class 100) aseptic fill & finish under an ISO 13485:2016 quality management system
  • Microfluidics Suitable at a 0.04 µm final cut-off — a separate 0.01 µm MPS Grade line is available on request
  • pH, glucose concentration, salts, HEPES, and nutrient composition available on request
DCP-IMDMH-BR1X | Size: 500 mL and 1000 mL | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid, sterile-filtered
  • Glucose4500.000 mg/L
  • L-Glutamine584.000 mg/L
  • Sodium Pyruvate110.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)210–250 mOsm/kg H₂O
  • Endotoxin< 0.05 EU/mL
  • FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold-pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard 0.22 µm media fails

Conventional 0.22 µm-filtered media can carry subvisible particulates and mycoplasma-scale contaminants (mycoplasma range approximately 0.2–0.3 µm) that accumulate in microfluidic channels, corrupt biosensor signals, and shorten perfusion runs. FluxMPS™ IMDM, High Glucose + 25mM HEPES w/o Bicarbonate, Phenol Red is re-engineered for these microphysiological systems.[1,2]

filter_alt

Microchannel-safe purity

A 0.04 µm final-polish filter targets subvisible particulates per USP <788> Method 1 (light obscuration), preventing the microchannel accumulation and clogging that degrade organ-on-a-chip experiments.

target

Total metabolic control

A defined carbon-source and nutrient backbone (glucose, L-glutamine, sodium pyruvate) supports Warburg-effect and metabolic-flux studies where standard serum-rich media confound the readout.

water_drop

Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon (TOC) control, supporting a clean baseline for sensitive assays.

visibility

Low background for imaging

Ultra-low particulate baseline reduces optical scatter for confocal microscopy, biosensors, and TEER measurements.

science

Rich, stable nutrient profile

IMDM's enriched amino-acid and vitamin profile, selenium, and sodium pyruvate are released on a per-batch basis for lot-to-lot consistency.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrient composition can be adjusted on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

The FluxMPS™ purity architecture is built around a four-stage membrane train — two dedicated 0.1 µm prefilter / 0.04 µm final-filter pairs run in series (0.1 µm ×2 + 0.04 µm ×2) — positioning DCP-IMDMH-BR1X as a ready-to-use, ultra-clean medium for microfluidic and organ-on-a-chip work at a purity level conventional 0.22 µm media do not typically reach.[2,3]

  1. 1

    0.1 µmPrefiltration I

    Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µmFinal filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µmPrefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µmFinal filtration II — Polish

    Ultimate polishing filter; aseptic fill performed under an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

5×
cleaner than 0.22 µm media (particulate count)
0.04
µm final-polish membrane
Sterility & mycoplasma assurance. No bacterial or fungal growth after 14 days of incubation per USP <71> specification. Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are approximately 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid (DCP-IMDMH-BR1X) ? HEPES-buffered, without sodium bicarbonate, without phenol red ? Quadruple-stage filtration (0.1 μm ×2 + 0.04 μm ×2) ultra-filtered IMDM cell culture media for organ-on-a-chip, microphysiological system (MPS) and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture — two 0.1 µm prefiltration passes paired with two 0.04 µm final-filtration passes for ultra-low-particulate, microchannel-safe media.
© Diagnocine® — DCP-IMDMH-BR1X
Applications

Built for microphysiological & organ-on-a-chip research

IMDM, High Glucose + 25mM HEPES w/o Bicarbonate, Phenol Red supports rapidly proliferating, high-density cultures — from erythroid progenitors, macrophages, and B/T lymphocytes to hybridomas — and is well suited to perfused MPS, metabolic, and imaging applications.[1,4]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors and robotic perfusion platforms, an optional 10 nm (0.01 µm) ultra-filtered MPS Grade variant is available to push particulate exclusion beyond this Microfluidics Suitable formulation's 0.04 µm polish.

  • Total particulate exclusion for narrow microchannels and high-resolution optics
  • Valve & sensor protection across long unattended runs
  • Extended perfusion stability for multi-week organ-on-a-chip experiments

Inquiry Required: the optional 0.01 µm (10 nm) MPS Grade variant is made to order — contact support@diagnocine.com to request it.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low-particulate fluid for perfused chips and barrier models.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon-source backbone for tumor-metabolism studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Consistent nutrient profile for differentiated iPSC cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Low-background medium for vessel-on-chip and primary cells.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Clean, bicarbonate-free, phenol-red-free baseline for tracer and respirometry workflows.

¹³C tracingSeahorse XFNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline for long-term imaging and biosensing.

ConfocalBiosensorsTEER
Technical Specifications

Technical specifications

Representative specifications for DCP-IMDMH-BR1X. Values are confirmed per lot on the Certificate of Analysis. Available in 500 mL and 1000 mL sizes.

Physical & Chemical Parameters
Parameter Specification
Formulation Iscove's Modified Dulbecco's Medium (IMDM), 1X liquid, high glucose, with L-glutamine, HEPES (25 mM), calcium, magnesium and sodium pyruvate; without sodium bicarbonate and without phenol red
Appearance Colorless, clear solution
pH USP <791> 7.4
Osmolality USP <785> 210–250 mOsm/kg H₂O
Glucose 4500.000 mg/L
L-Glutamine 584.000 mg/L
Sodium Pyruvate 110.000 mg/L
Phenol Red Not added
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> Filtered in a sterile environment. No bacterial or fungal growth observed after 14 days of incubation, per USP specification.
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Meets USP <788> Method 1 (light obscuration) limits
Particulate ≥25 µm USP <788> Meets USP <788> Method 1 (light obscuration) limits
Water purity Ultrapure Type 1 (18.2 MΩ·cm)
Manufacturing std. ISO ISO 13485:2016; 21 CFR Part 820 (QMSR) aligned
Fill environment ISO Class 5 (Class 100) aseptic
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Avoid repeated freeze-thaw
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold-pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (no sodium bicarbonate) — validate per cell line
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell-culture grade, traceable lots
Traceability Full lot traceability with CoA
Manufacturing QMS ISO 13485:2016 certified facility
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Batch production, ISO Class 5 fill & finish
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete defined formulation of DCP-IMDMH-BR1X, released on a per-batch basis. Total: 40 components across 4 formulation categories (Inorganic Salts, Amino Acids, Vitamins, Others).

INORGANIC SALTS
Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 219.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 330.000
Potassium nitrate 7757-79-1 0.076
Sodium chloride 7647-14-5 4505.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Sodium selenite 10102-18-8 0.0173
AMINO ACIDS
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Alanine 56-41-7 25.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Asparagine 70-47-3 25.000
L-Aspartic acid 56-84-8 30.000
L-Cystine dihydrochloride 30925-07-6 91.240
L-Glutamic acid 56-86-0 75.000
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 104.800
L-Leucine 61-90-5 104.800
L-Lysine hydrochloride 657-27-2 146.200
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Proline 147-85-3 40.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.200
L-Tryptophan 73-22-3 16.000
L-Tyrosine disodium salt 69847-45-6 104.200
L-Valine 72-18-4 93.600
VITAMINS / OTHERS
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Biotin 58-85-5 0.013
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
Vitamin B12 68-19-9 0.013
i-Inositol 87-89-8 7.200
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Sodium pyruvate 113-24-6 110.000
Need a custom formulation? pH, glucose, salts, HEPES, and nutrient composition can be adjusted — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

All final packaging, quality assurance, and testing for DCP-IMDMH-BR1X are completed at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Manufactured under an ISO 13485:2016 quality management system, 21 CFR Part 820 (QMSR) aligned.

water_drop

Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm Ultrapure Type 1 water for trace-metal and organic-carbon control.

biotech

ISO Class 5 Fill & Finish

Final 0.04 µm polish and aseptic fill performed in an ISO Class 5 (Class 100) environment.

assignment

Batch-Level Precision

Batch production with per-lot release testing for lot-to-lot consistency and traceability.

Endotoxin — USP <85> BET

Bacterial endotoxin testing (LAL assay) to a < 0.05 EU/mL release specification.

Particulate — USP <788> Method 1

Subvisible particulate matter controlled to USP <788> Method 1 (light obscuration) limits.

Osmolality — USP <785>

Osmolality verified per lot within the specified range.

Documentation / CoA

A Certificate of Analysis accompanies every lot with measured QC values.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-IMDMH-BR1X compares

DCP-IMDMH-BR1X versus conventional 0.22 µm-filtered IMDM and a standard 0.22 µm-filtered DMEM-class alternative.

Parameter DCP-IMDMH-BR1X (FluxMPS™) Conventional IMDM (0.22 µm) Standard DMEM-class (0.22 µm)
Grade Microfluidics Suitable (0.04 µm final) Standard grade (0.22 µm) Standard grade (0.22 µm)
Formulation Iscove-enriched amino acids/vitamins + selenium, potassium nitrate; HEPES-buffered, no bicarbonate, no phenol red IMDM base DMEM base
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Type 1/2 Type 1/2
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle Limited Limited
Custom formulation check_circle Limited Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about DCP-IMDMH-BR1X for microphysiological and standard cell-culture workflows.

Yes. DCP-IMDMH-BR1X is nano-filtered to a 0.04 µm final polish and formulated with Ultrapure Type 1 water specifically to minimize subvisible particulates that clog microchannels and corrupt biosensor signals, making it well suited to OoC, MPS, and microfluidic perfusion. This is a Microfluidics Suitable formulation; a separate 0.01 µm MPS Grade line is available on request for automated bioreactors.
 
In DCP-IMDMH-BR1X, phenol red is omitted to give you control over your culture conditions. No pH indicator is present, which removes a known source of optical/estrogenic interference; monitor pH externally if needed. Review the literature for the requirements of your specific cell line.
This formulation is HEPES-buffered (25 mM) and does not contain sodium bicarbonate, so standard bicarbonate/CO₂ buffering is not provided. It has reduced CO₂ dependence; validate the appropriate CO₂ environment for your specific cell line and culture system.
Yes. IMDM contains no proteins, lipids, or growth factors, so it is typically supplemented with serum or serum-free additives. When supplementing with serum, growth factors, or other protein-containing additives, filter through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use 0.04 µm filters for these additions, as they will strip essential serum proteins and lipoproteins.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by LAL assay per USP <85> (BET); assay sensitivity is 0.005 EU/mL. Every batch is tested before release.
Yes. A CoA accompanies every batch of DCP-IMDMH-BR1X with measured QC values such as appearance, pH, osmolality, sterility, and endotoxin. Request one at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed literature supporting the use of enriched, ultra-filtered media in microphysiological and metabolic research.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell. 2018;22(3):310–324.doi:10.1016/j.stem.2018.02.011
  3. Halldorsson S, et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231.doi:10.1016/j.bios.2014.07.029
  4. Iscove NN, Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978;147(3):923–933.doi:10.1084/jem.147.3.923
  5. Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029–1033.doi:10.1126/science.1160809
  6. Nikolaev M, et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020;585(7826):574–578.doi:10.1038/s41586-020-2724-8
  7. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90.doi:10.1023/A:1022913015916
  8. Booth R, Kim H. Characterization of a microfluidic in vitro model of the blood-brain barrier. Lab Chip. 2012;12(10):1784–1792.doi:10.1039/c2lc40094d
  9. Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442(7101):368–373.doi:10.1038/nature05058
  10. Low LA, Mummery C, Berridge BR, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361.doi:10.1038/s41573-020-0079-3

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