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FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine: 1X Liquid

Product#: DCP-IMDMH-Q1X
$44.00
DCP-IMDMH-Q1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine

A Microfluidics Suitable, high-glucose Iscove's Modified Dulbecco's Medium (IMDM) re-engineered for microphysiological systems and microfluidic channels. Quadruple-stage ultra-filtration (0.1 µm ×2 + 0.04 µm ×2) targets the 0.04 µm particulate range, well below conventional 0.22 µm formulations, with an endotoxin release specification of < 0.05 EU/mL and Ultrapure Type 1 water.

  • High-glucose (4500 mg/L) Iscove's Modified Dulbecco's Medium supplemented with 25 mM HEPES; formulated without L-glutamine so the end user controls glutamine supplementation
  • Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2), four validated passes, for ultra-low particulate, microchannel-safe media
  • Endotoxin release specification < 0.05 EU/mL by USP <85> (Bacterial Endotoxins Test), tested per manufacturing batch
  • Sodium pyruvate (110 mg/L) included as an alternate carbon source and antioxidant buffer for metabolically sensitive cultures
  • Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize trace-metal and organic-carbon background
  • ISO Class 5 (Class 100) aseptic fill & finish under an ISO 13485:2016 quality management system
  • pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
DCP-IMDMH-Q1X Size: 500 mL, 1000 mL Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine: 1X Liquid, sterile-filtered
  • Glucose4500.000 mg/L
  • L-GlutamineNot included
  • Sodium Pyruvate110.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)210-250 mOsm/kg H2O
  • Endotoxin< 0.05 EU/mL
  • FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
  • Storage2-8°C, protected from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold-pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard 0.22 µm media fails

Conventional 0.22 µm-filtered media can carry subvisible particulates and mycoplasma-scale contaminants (mycoplasma range 0.2–0.3 µm in diameter) that accumulate in microfluidic channels, corrupt biosensor signals, and shorten perfusion runs. FluxMPS™ IMDM, High Glucose + 25mM HEPES w/o L-Glutamine is re-engineered for these microphysiological systems.[1,2]

filter_alt

Microchannel-safe purity

A 0.04 µm final-polish filter targets subvisible particulates per USP <788> Method 1 (light obscuration), reducing the microchannel accumulation and clogging that can degrade organ-on-a-chip experiments.

target

Total metabolic control

A defined carbon-source and nutrient backbone, with sodium pyruvate as an alternate substrate, supports Warburg-effect and metabolic-flux studies where standard serum-rich media confound the readout.

water_drop

Ultrapure-grade water

Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), reducing trace-metal and organic-carbon background in the finished medium.

visibility

Low background for imaging

Low-particulate fluid reduces the risk of scatter artifacts in confocal microscopy, biosensor readouts, and TEER measurements. This formulation contains phenol red and riboflavin, both of which contribute their own optical background; phenol-red-free custom formulations are available on request.

science

Rich, stable nutrient profile

IMDM's enriched amino-acid and vitamin profile, selenium, and sodium pyruvate are released on a micro-batch basis for lot-to-lot consistency.

tune

Customization on demand

pH, glucose, salts, HEPES, and nutrient composition can be adjusted on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

The FluxMPS™A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.[2,3]

  1. 1

    0.1 µmPrefiltration I

    Large particulate and aggregate removal that extends downstream filter life and protects the first 0.04 µm cartridge.

  2. 2

    0.04 µmFinal filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µmPrefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge for redundant assurance.

  4. 4

    0.04 µmFinal filtration II — Polish

    Ultimate polishing filter; ISO Class 5 (Class 100) aseptic fill & finish.

Performance vs. conventional media

0.04
µm final-polish membrane
4
validated filtration passes
Sterility & mycoplasma assurance. No bacterial or fungal growth after 14 days of incubation per USP <71> specification. Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma range 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Iscove's Modified Dulbecco's Medium (IMDM), High Glucose with 25mM HEPES w/o L-Glutamine 1X Liquid (DCP-IMDMH-Q1X) - Quadruple-stage filtration (0.1 micron x2 + 0.04 micron x2) ultra-filtered IMDM cell culture media for organ-on-a-chip, microphysiological system (MPS) and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture — two 0.1 µm prefiltration passes, each paired with a dedicated 0.04 µm final-filtration pass, for ultra-low-particulate, microchannel-safe media.
© Diagnocine® — DCP-IMDMH-Q1X
Applications

Built for microphysiological & organ-on-a-chip research

IMDM, High Glucose + 25mM HEPES w/o L-Glutamine supports rapidly proliferating, high-density cultures — from erythroid progenitors, macrophages, and B/T lymphocytes to hybridomas — and is well suited to perfused MPS, metabolic, and imaging applications.[1,4]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors and robotic perfusion platforms, an optional Microfluidics-plus, 10 nm (0.01 µm) ultra-filtered MPS Grade variant is available, adding 0.02 µm and 0.01 µm stages beyond the standard 0.04 µm polish.

  • Total particulate exclusion for narrow microchannels and high-resolution optics
  • Valve & sensor protection across long unattended runs
  • Extended perfusion stability for multi-week organ-on-a-chip experiments

Inquiry Required: the optional 0.01 µm (10 nm) MPS Grade variant is made to order — contact support@diagnocine.com to request it.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low-particulate fluid for perfused chips and barrier models.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon-source backbone for tumor-metabolism studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Consistent nutrient profile for differentiated iPSC cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Low-background medium for vessel-on-chip and primary cells.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Clean baseline for tracer and respirometry workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low-particulate fluid for long-term imaging workflows.

ConfocalBiosensorsTEER
Technical Specifications

Technical specifications

Representative specifications for DCP-IMDMH-Q1X. Values are confirmed per lot on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation IMDM base with sodium bicarbonate, phenol red, 25 mM HEPES, calcium, magnesium, glucose (4500 mg/L) and sodium pyruvate (110 mg/L); L-glutamine not included
Appearance Orange-red-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 210-250 mOsm/kg H2O
Glucose 4500.000 mg/L
L-Glutamine Not included
Sodium Pyruvate 110.000 mg/L
Phenol Red 15.000 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see §Manufacturing & Compliance)
Sterility USP <71> Filtered in a sterile environment. No bacterial or fungal growth observed after 14 days of incubation, per USP specification.
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Meets USP <788> Method 1 (light obscuration) limits
Particulate ≥25 µm USP <788> Meets USP <788> Method 1 (light obscuration) limits
Water purity Ultrapure Type 1 (18.2 MΩ·cm)
Manufacturing std. ISO ISO 13485:2016; 21 CFR Part 820 (QMSR) aligned
Fill environment ISO Class 5 (Class 100) aseptic
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2-8°C, protected from light
Freeze-thaw Avoid repeated freeze-thaw
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold-pack
CO2 requirement ∼8% CO2 recommended (HEPES-supplemented, sodium bicarbonate-buffered system; calculated per Henderson-Hasselbalch from 36 mM NaHCO3 to maintain pH 7.4)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell-culture grade, traceable lots
Traceability Full lot traceability with CoA
Manufacturing QMS ISO 13485:2016 certified facility
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, ISO Class 5 fill & finish
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete defined formulation of DCP-IMDMH-Q1X, released on a per-lot basis. All 41 components and their mg/L values are reproduced from the validated formulation.

INORGANIC SALTS
Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 219.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 330.000
Potassium nitrate 7757-79-1 0.076
Sodium bicarbonate 144-55-8 3024.000
Sodium chloride 7647-14-5 4505.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Sodium selenite 10102-18-8 0.0173
AMINO ACIDS
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Alanine 56-41-7 25.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Asparagine 70-47-3 25.000
L-Aspartic acid 56-84-8 30.000
L-Cystine dihydrochloride 30925-07-6 91.240
L-Glutamic acid 56-86-0 75.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 104.800
L-Leucine 61-90-5 104.800
L-Lysine hydrochloride 657-27-2 146.200
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Proline 147-85-3 40.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.200
L-Tryptophan 73-22-3 16.000
L-Tyrosine disodium salt 69847-45-6 104.200
L-Valine 72-18-4 93.600
VITAMINS / OTHERS
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Biotin 58-85-5 0.013
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
Vitamin B12 68-19-9 0.013
i-Inositol 87-89-8 7.200
OTHERS
D-Glucose 50-99-7 4500.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 15.000
Sodium pyruvate 113-24-6 110.000
Need a custom formulation? pH, glucose, salts, HEPES, and nutrient composition can be adjusted — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

All final packaging, quality assurance, and testing for DCP-IMDMH-Q1X are completed at the Diagnocine R&D and Quality Testing Center; customization and assembly are performed at Diagnocine Precision, Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Manufactured under an ISO 13485:2016 quality management system, 21 CFR Part 820 (QMSR) aligned.

water_drop

Ultrapure Type 1 Water

Formulated with 18.2 MΩ·cm Ultrapure Type 1 water for low trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Final 0.04 µm polish and aseptic fill performed in an ISO Class 5 (Class 100) environment.

assignment

Micro-Batch Precision

Micro-batch production with per-lot release testing for lot-to-lot consistency and traceability.

Endotoxin USP <85> BET

Bacterial endotoxin testing to a < 0.05 EU/mL release specification.

Particulate USP <788> Method 1

Subvisible particulate matter controlled to USP <788> Method 1 (light obscuration) limits.

Osmolality USP <785>

Osmolality verified per lot within the specified range.

Documentation / CoA

A Certificate of Analysis accompanies every lot with measured QC values.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-IMDMH-Q1X compares

DCP-IMDMH-Q1X versus conventional 0.22 µm-filtered IMDM and a standard 0.22 µm-filtered DMEM-class alternative.

Parameter DCP-IMDMH-Q1X (FluxMPS™) Conventional IMDM (0.22 µm) Standard DMEM-class (0.22 µm)
Grade Microfluidics Suitable Not applicable (standard 0.22 µm filtered) Not applicable (standard 0.22 µm filtered)
Formulation enrichment Iscove-enriched amino acids/vitamins + selenium, potassium nitrate; L-glutamine-free IMDM base DMEM base
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Number of filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Type 1/2 Type 1/2
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle Limited Limited
Custom formulation check_circle Limited Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about DCP-IMDMH-Q1X for microphysiological and standard cell-culture workflows.

Yes. DCP-IMDMH-Q1X is filtered to a 0.04 µm final polish across four validated passes and formulated with Ultrapure Type 1 water specifically to minimize subvisible particulates that clog microchannels and corrupt biosensor signals, making it well suited to OoC, MPS, and microfluidic perfusion. It is a Microfluidics Suitable product; the separate 0.01 µm MPS Grade line is available for automated bioreactors on request.
The FluxMPS™ Quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) carries the medium through two dedicated prefilter-plus-final-filter pairs to a 0.04 µm final polish, well below the 0.22 µm cut-off of conventional media, and provides a 0.1 µm mycoplasma-retentive filtration step (not tested per lot).
In DCP-IMDMH-Q1X, L-glutamine is omitted to give you control over your culture conditions. To adapt it, supplement with fresh L-glutamine or a stable dipeptide (e.g., L-alanyl-L-glutamine) at your preferred concentration just before use. Review the literature for the requirements of your specific cell line.
Yes. This formulation combines 25 mM HEPES with 36 mM sodium bicarbonate buffering. Calculated per Henderson-Hasselbalch from the stated bicarbonate concentration, an atmosphere of approximately 8% CO2 is recommended to maintain pH 7.4; the HEPES component provides additional buffering capacity and tolerance for brief excursions outside a controlled incubator.
Yes. IMDM contains no proteins, lipids, or growth factors, so it is typically supplemented with serum or serum-free additives. When adding serum or other protein-containing supplements, filter with a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for supplement filtration, as it will strip serum of the components that make it functional. Defined, protein-free additions may use a 0.1 µm membrane.
The release specification is < 0.05 EU/mL, verified by LAL assay per USP <85> (Bacterial Endotoxins Test), assay sensitivity 0.005 EU/mL. Endotoxin is controlled per manufacturing batch; every batch is tested before release and must meet this specification, and the result is reported on the Certificate of Analysis.
Yes. A CoA accompanies every lot of DCP-IMDMH-Q1X with measured QC values such as appearance, pH, osmolality, sterility, and endotoxin, along with lot number and expiry. Request one at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed literature supporting the use of enriched, ultra-filtered media in microphysiological and metabolic research.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772.doi:10.1038/nbt.2989
  2. Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development. Cell Stem Cell. 2018;22(3):310-324.doi:10.1016/j.stem.2018.02.011
  3. Halldorsson S, et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218-231.doi:10.1016/j.bios.2014.07.029
  4. Iscove NN, Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978;147(3):923-933.doi:10.1084/jem.147.3.923
  5. Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029-1033.doi:10.1126/science.1160809
  6. Nikolaev M, et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature. 2020;585(7826):574-578.doi:10.1038/s41586-020-2724-8
  7. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75-90.doi:10.1023/A:1022913015916
  8. Booth R, Kim H. Characterization of a microfluidic in vitro model of the blood-brain barrier. Lab Chip. 2012;12(10):1784-1792.doi:10.1039/c2lc40094d
  9. Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442(7101):368-373.doi:10.1038/nature05058
  10. Low LA, Mummery C, Berridge BR, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361.doi:10.1038/s41573-020-0079-3

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