DMEM, High Glucose without Sodium Chloride

Catalog No.: DCP-DMEM-S1X

Size: 500 mL, 1000 mL
Sterile: Filtered 0.1-micron membrane TWICE, 0.04-micron membrane ONCE

[+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate
[-] Sodium Chloride

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modifications of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentrations of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1 g/L of glucose and was originally used to culture embryonic mouse cells. The low glucose level is suitable for growing cells that may be adversely affected by high glucose concentrations, such as certain primary cells, stem cells, or transformed cell lines.

High glucose may induce ROS, protein modifications, glycosylations and other signaling in certain cell types and in such case, low glucose DMEM may be preferred media. As for glucose contents, DMEM High Glucose has 4.5g/L; MEM has 1g/L; RPMI has 4g/L. Different glucose concentration may affect cell signaling pathways.
 
This product is 1X Dulbecco's Modified Eagle Medium with 1.0 gms glucose per liter, L-glutamine, sodium pyruvate, sodium bicarbonate. It does not contain HEPES. Users should review the literature for recommendations regarding medium supplementation and physiological growth requirements for different cell lines and conditions.

CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
 

Composition:

Ingredients	mg/L	Ingredients	mg/L
INORGANIC SALTS		AMINO ACIDS
Calcium chloride dihydrate	265.000	Glycine	30.000
Ferric nitrate nonahydrate	0.100	L-Arginine hydrochloride	84.000
Magnesium sulphate anhydrous	97.720	L-Cystine dihydrochloride	62.570
Potassium chloride	400.000	L-Glutamine	584.000
Sodium bicarbonate	3700.000	L-Histidine hydrochloride monohydrate	42.000
Sodium dihydrogen phosphate anhydrous	109.000	L-Isoleucine	105.000
VITAMINS		L-Leucine	105.000
Choline chloride	4.000	L-Lysine hydrochloride	146.000
D-Ca-Pantothenate	4.000	L-Methionine	30.000
Folic acid	4.000	L-Phenylalanine	66.000
Nicotinamide	4.000	L-Serine	42.000
Pyridoxal hydrochloride	4.000	L-Threonine	95.000
Riboflavin	0.400	L-Tryptophan	16.000
Thiamine hydrochloride	4.000	L-Tyrosine Disodium Salt dihydrate	103.790
i-Inositol	7.200	L-Valine	94.000
OTHERS
D-Glucose	4500.000
Phenol red sodium salt	15.900
Sodium pyruvate	110.000

 

Quality Control

• Apperance: Red-colored, clear solution.
• pH (at 1X concentration): 7.4
• Osmolality in mOsm/Kg H2O (at 1X concentration): 310.00 - 350.00
• Sterility: Filtered 0.1 micron twice and 0.04 micron once in a sterile environment. No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
• Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for morphology and quantitatively by estimating the cell counts and comparing it with a control medium.
• Endotoxin Content: NMT 1EU/ml

Storage and Shelf Life

• Storage: 2-8°C, away from bright light.
• Shelf life: 12 months.
• Use before the expiry date given on the product label.

* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented.  The smallest size mycoplasma type can be about 0.2 microns.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA