DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
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Human Ubiquitin-like 1-activating Enzyme E1B (UBA2)
Cat.No.: JNB-JL17959
Size: 96TSpecies: HumanAssay Type: SandwichSensitivity: 0.07ng/mLDetection Range: 0.156-10ng/mLAlternative Names: UBLE1B;SAE2; ARX; SUMO1 Activating Enzyme Subunit 2; Anthracycline-associated resistance ARX; Ubiquitin Like Modifier Activating Enzyme 2; SUMO-activating enzyme subunit 2;EC:2.3.2.- ;Ubiquitin-like modifier-activating enzyme 2;Ubiquitin-like 1-activating enzyme E1B;HRIHFB2115Research Areas: Cell BiologyAssay Time: 2h45mAbbreviation: UBA2Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UBA2. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Human UBA2 in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Human UBA2 in the samples by comparing the OD of the samples to the standard curve.Specificity: This kit recognizes Human UBA2 in samples. No significant cross-reactivity or interference between Human UBA2 and analogues was observed.Recovery rate: 80%-120%Precision: Intra-CV and Inter-CV <10% Shipping: Ice Bag