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Human Tumour necrosis factor related weak inducer of apoptosis, TWEAK ELISA Kit
Product#: BTL-E1820Hu
$686.40
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Human Tumour necrosis factor related weak inducer of apoptosis, TWEAK ELISA Kit
Cat. No#: BTL-E1820Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 5.51mg/L
Detection range: 10-4000mg/L
Sample type: Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue
Reactive with: Human
Product overview
Full product name
Tumour necrosis factor related weak inducer of apoptosis
SKU#.: ? BTL- |
E1820Hu |
Assay type |
Sandwich |
Size |
5.51mg/L |
Detection range |
10-4000mg/L |
Sample type |
Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue |
Species |
Human |
Storage |
2-8°C |
Assay time |
1h 30m |
Background |
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. |
Research area |
Cell Biology;Cancer |
Target protein |
TWEAK |
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1820Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400mg/L | 2.106 | 2.214 | 2.161 |
| 2.322 | |||
| 1200mg/L | 1.852 | 1.782 | 1.729 |
| 1.712 | |||
| 600mg/L | 1.133 | 1.195 | 1.142 |
| 1.257 | |||
| 300mg/L | 0.725 | 0.692 | 0.639 |
| 0.66 | |||
| 150mg/L | 0.439 | 0.439 | 0.386 |
| 0.44 | |||
| 0mg/L | 0.047 | 0.053 | 0 |
| 0.059 |
Precision
We measured random samples of E1820Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1234.5 | 74.3 | 6 |
| Intra-assay | 2 | 18 | 270.1 | 14.5 | 5.4 |
| Intra-assay | 3 | 18 | 606.6 | 42.3 | 7 |