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Human Sphingosine 1-phosphate Receptor Type 3, S1P3 ELISA Kit
Product#: BTL-E6217Hu
$686.40
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Human Sphingosine 1-phosphate Receptor Type 3, S1P3 ELISA Kit
Cat. No#: BTL-E6217Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 7.66ng/L
Detection range: 15-3000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Sphingosine 1-Phosphate Receptor Type 3
SKU#.: ? BTL- |
E6217Hu |
Assay type |
Sandwich |
Size |
7.66ng/L |
Detection range |
15-3000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. |
UniProt accession |
42,250 |
GeneID |
1903 |
Synonyms |
Endothelial differentiation G-protein coupled receptor 3;S1P receptor 3;S1P receptor Edg-3;S1P3;S1PR3;Sphingosine 1-phosphate receptor 3;Sphingosine 1-phosphate receptor Edg-3 |
Gene names |
S1PR3 |
Research area |
Signal Transduction |
Target protein |
S1PR3 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6217Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.039 | 2.17 | 2.117 |
| 2.3 | |||
| 800ng/L | 1.481 | 1.409 | 1.356 |
| 1.337 | |||
| 400ng/L | 0.765 | 0.821 | 0.768 |
| 0.877 | |||
| 200ng/L | 0.493 | 0.514 | 0.462 |
| 0.536 | |||
| 100ng/L | 0.22 | 0.23 | 0.178 |
| 0.241 | |||
| 0ng/L | 0.07 | 0.052 | 0 |
| 0.035 |
Precision
We measured random samples of E6217Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1211 | 46.28 | 3.8 |
| Intra-Assay | 2 | 18 | 204.3 | 9.69 | 4.7 |
| Intra-Assay | 3 | 18 | 454.8 | 27.56 | 6.1 |