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Human Sparc-related Modular Calcium-binding Protein 1, SMOC1 ELISA Kit
Product#: BTL-E4583Hu
$686.40
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Human Sparc-related Modular Calcium-binding Protein 1, SMOC1 ELISA Kit
Cat. No#: BTL-E4583Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 14.85ng/L
Detection range: 30-9000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Sparc-Related Modular Calcium-Binding Protein 1
SKU#.: ? BTL- |
E4583Hu |
Assay type |
Sandwich |
Size |
14.85ng/L |
Detection range |
30-9000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation. |
UniProt accession |
48,163 |
GeneID |
64093 |
Synonyms |
Secreted modular calcium-binding protein 1;SMOC 1;SMOC1;SMOC-1;SPARC-related modular calcium-binding protein 1 |
Gene names |
SMOC1 |
Research area |
Others |
Target protein |
SMOC1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4583Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4800ng/L | 2.035 | 2.142 | 2.083 |
| 2.248 | |||
| 2400ng/L | 1.412 | 1.475 | 1.416 |
| 1.537 | |||
| 1200ng/L | 0.917 | 0.897 | 0.838 |
| 0.877 | |||
| 600ng/L | 0.526 | 0.539 | 0.48 |
| 0.551 | |||
| 300ng/L | 0.267 | 0.277 | 0.218 |
| 0.286 | |||
| 0ng/L | 0.066 | 0.059 | 0 |
| 0.052 |
Precision
We measured random samples of E4583Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 817.3 | 52.65 | 6.4 |
| Intra-Assay | 2 | 18 | 1209.5 | 64.81 | 5.4 |
| Intra-Assay | 3 | 18 | 2830.3 | 106.24 | 3.8 |