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Human Serine Threonine-protein Phosphatase with EF-Hands 1, PPEF1 ELISA Kit
Product#: BTL-E6155Hu
$686.40
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Human Serine Threonine-protein Phosphatase with EF-Hands 1, PPEF1 ELISA Kit
Cat. No#: BTL-E6155Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 9.16ng/L
Detection range: 20-3500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Serine/threonine-protein phosphatase with EF-hands 1
SKU#.: ? BTL- |
E6155Hu |
Assay type |
Sandwich |
Size |
9.16ng/L |
Detection range |
20-3500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
May have a role in the recovery or adaptation response of photoreceptors. May have a role in development. |
UniProt accession |
75,792 |
GeneID |
5475 |
Synonyms |
PP7;PPEF;PPEF1;PPEF-1;PPP7C;PPP7CA;Protein phosphatase with EF calcium-binding domain;Serine/threonine-protein phosphatase 7;Serine/threonine-protein phosphatase with EF-hands 1; |
Gene names |
PPEF1 |
Research area |
Others |
Target protein |
PPEF1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6155Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 1.741 | 1.734 | 1.698 |
| 1.726 | |||
| 1000ng/L | 1.087 | 1.05 | 1.014 |
| 1.013 | |||
| 500ng/L | 0.61 | 0.511 | 0.475 |
| 0.412 | |||
| 250ng/L | 0.276 | 0.282 | 0.246 |
| 0.288 | |||
| 125ng/L | 0.091 | 0.082 | 0.046 |
| 0.073 | |||
| 0ng/L | 0.046 | 0.036 | 0 |
| 0.026 |
Precision
We measured random samples of E6155Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1692.76 | 70.2 | 4.15 |
| Intra-Assay | 2 | 18 | 673.99 | 37.74 | 5.6 |
| Intra-Assay | 3 | 18 | 759.21 | 36.2 | 4.77 |