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Human Serine Threonine-protein Phosphatase PP1-beta Catalytic Subunit, PPP1CB ELISA Kit
Product#: BTL-E6154Hu
$686.40
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Human Serine Threonine-protein Phosphatase PP1-beta Catalytic Subunit, PPP1CB ELISA Kit
Cat. No#: BTL-E6154Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 4.92ng/L
Detection range: 10-2000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
SKU#.: ? BTL- |
E6154Hu |
Assay type |
Sandwich |
Size |
4.92ng/L |
Detection range |
10-2000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). |
UniProt accession |
37,187 |
GeneID |
5500 |
Synonyms |
HEL-S-80p;MP;NSLH2;PP1B;PP-1B;PP1c;PPP1beta;PPP1CB;PPP1CD;Serine/threonine-protein phosphatase PP1-beta catalytic subunit |
Gene names |
PPP1CB |
Research area |
Others |
Target protein |
PPP1CB |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6154Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.237 | 2.272 | 2.209 |
| 2.306 | |||
| 600ng/L | 1.254 | 1.234 | 1.172 |
| 1.215 | |||
| 300ng/L | 0.784 | 0.777 | 0.714 |
| 0.769 | |||
| 150ng/L | 0.431 | 0.494 | 0.432 |
| 0.558 | |||
| 75ng/L | 0.282 | 0.269 | 0.206 |
| 0.255 | |||
| 0ng/L | 0.07 | 0.063 | 0 |
| 0.055 |
Precision
We measured random samples of E6154Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 406.27 | 21.26 | 5.23 |
| Intra-Assay | 2 | 18 | 914.28 | 22.39 | 2.45 |
| Intra-Assay | 3 | 18 | 289.35 | 19.06 | 6.59 |