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Human Sarcoplasmic Reticulum Histidine-rich Calcium-binding Protein, HRC ELISA Kit
Product#: BTL-E2744Hu
$686.40
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Human Sarcoplasmic Reticulum Histidine-rich Calcium-binding Protein, HRC ELISA Kit
Cat. No#: BTL-E2744Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.019ng/ml
Detection range: 0.05-15ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Sarcoplasmic reticulum histidine-rich calcium-binding protein
SKU#.: ? BTL- |
E2744Hu |
Assay type |
Sandwich |
Size |
0.019ng/ml |
Detection range |
0.05-15ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
May play a role in the regulation of calcium sequestration or release in the SR of skeletal and cardiac muscle. |
UniProt accession |
80,244 |
GeneID |
3270 |
Synonyms |
HRC;Sarcoplasmic reticulum histidine-rich calcium-binding protein |
Gene names |
HRC |
Research area |
Cell Biology |
Target protein |
HRC |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2744Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.389 | 2.461 | 2.426 |
| 2.533 | |||
| 4ng/ml | 1.323 | 1.461 | 1.426 |
| 1.599 | |||
| 2ng/ml | 0.938 | 0.908 | 0.873 |
| 0.877 | |||
| 1ng/ml | 0.419 | 0.444 | 0.409 |
| 0.469 | |||
| 0.5ng/ml | 0.214 | 0.217 | 0.182 |
| 0.22 | |||
| 0ng/ml | 0.036 | 0.035 | 0 |
| 0.034 |
Precision
We measured random samples of E2744Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.32 | 0.08 | 6.35 |
| Intra-Assay | 2 | 18 | 3.28 | 0.11 | 3.3 |
| Intra-Assay | 3 | 18 | 2.41 | 0.13 | 5.21 |