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Human SPARC ELISA Kit
Product#: BTL-E6206Hu
$686.40
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Human SPARC ELISA Kit
Cat. No#: BTL-E6206Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 9.48ng/mL
Detection range: 20-7000ng/mL
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
SPARC
SKU#.: ? BTL- |
E6206Hu |
Assay type |
Sandwich |
Size |
9.48ng/mL |
Detection range |
20-7000ng/mL |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity. |
UniProt accession |
34,632 |
GeneID |
6678 |
Synonyms |
Basement-membrane protein 40;BM-40;OI17;ON;ONT;Osteonectin;Secreted protein acidic and rich in cysteine;SPARC |
Gene names |
SPARC |
Research area |
Others |
Target protein |
SPARC |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4000ng/mL | 2.044 | 2.155 | 2.099 |
| 2.265 | |||
| 2000ng/mL | 1.352 | 1.328 | 1.273 |
| 1.304 | |||
| 1000ng/mL | 0.779 | 0.762 | 0.707 |
| 0.746 | |||
| 500ng/mL | 0.533 | 0.466 | 0.41 |
| 0.399 | |||
| 250ng/mL | 0.223 | 0.225 | 0.169 |
| 0.227 | |||
| 0ng/mL | 0.051 | 0.056 | 0 |
| 0.06 |
Precision
We measured random samples of E6206Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 474.95 | 25.96 | 5.47 |
| Intra-Assay | 2 | 18 | 1085.88 | 70.13 | 6.46 |
| Intra-Assay | 3 | 18 | 2992.7 | 140.91 | 4.71 |