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Human Immunosuppressive Acidic Protein, IAP ELISA Kit
Product#: BTL-E0425Hu
$686.40
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Human Immunosuppressive Acidic Protein, IAP ELISA Kit
Cat. No#: BTL-E0425Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 4.71μg/ml
Detection range: 10-2000ug/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
immunosuppressive acidic protein
SKU#.: ? BTL- |
E0425Hu |
Assay type |
Sandwich |
Size |
4.71μg/ml |
Detection range |
10-2000ug/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
UniProt accession |
20,333 |
GeneID |
10944 |
Synonyms |
IAP;IMAGE145052;Small acidic protein;SMAP |
Gene names |
SMAP |
Research area |
Cell Biology; Signal Transduction; Epigenetics and Nuclear Signaling; Cancer; Metabolism |
Target protein |
IAP |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0425Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200μg/ml | 2.221 | 2.179 | 2.134 |
| 2.137 | |||
| 600μg/ml | 1.509 | 1.503 | 1.458 |
| 1.497 | |||
| 300μg/ml | 0.705 | 0.698 | 0.653 |
| 0.691 | |||
| 150μg/ml | 0.421 | 0.388 | 0.343 |
| 0.354 | |||
| 75μg/ml | 0.14 | 0.177 | 0.133 |
| 0.214 | |||
| 0μg/ml | 0.056 | 0.045 | 0 |
| 0.034 |
Precision
We measured random samples of E0425Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 766.55 | 23.79 | 3.1 |
| Intra-Assay | 2 | 18 | 228.17 | 10.04 | 4.4 |
| Intra-Assay | 3 | 18 | 339.76 | 17.23 | 5.07 |