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Human Histone-lysine N-methyltransferase Ezh2, EZH2 ELISA Kit
Product#: BTL-E5422Hu
$686.40
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Human Histone-lysine N-methyltransferase Ezh2, EZH2 ELISA Kit
Cat. No#: BTL-E5422Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 9.93ng/L
Detection range: 20-4500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Histone-lysine N-methyltransferase EZH2
SKU#.: ? BTL- |
E5422Hu |
Assay type |
Sandwich |
Size |
9.93ng/L |
Detection range |
20-4500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
85,363 |
GeneID |
2146 |
Synonyms |
Enhancer of zeste homolog 2;ENX1;ENX-1;EZH2;EZH2b;Histone-lysine N-methyltransferase EZH2;KMT6;Lysine N-methyltransferase 6;WVS;WVS2 |
Gene names |
EZH2 |
Research area |
Others |
Target protein |
EZH2 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5422Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.403 | 2.346 | 2.313 |
| 2.289 | |||
| 1200ng/L | 1.355 | 1.397 | 1.364 |
| 1.439 | |||
| 600ng/L | 0.725 | 0.639 | 0.606 |
| 0.553 | |||
| 300ng/L | 0.294 | 0.312 | 0.279 |
| 0.329 | |||
| 150ng/L | 0.118 | 0.125 | 0.092 |
| 0.132 | |||
| 0ng/L | 0.023 | 0.033 | 0 |
| 0.043 |
Precision
We measured random samples of E5422Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 998.71 | 32.74 | 3.28 |
| Intra-Assay | 2 | 18 | 1321.91 | 42.12 | 3.19 |
| Intra-Assay | 3 | 18 | 527.76 | 23.78 | 4.51 |