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Human Gap Junction Beta-6 Protein, GJB6 ELISA Kit
Product#: BTL-E5322Hu
$686.40
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Human Gap Junction Beta-6 Protein, GJB6 ELISA Kit
Cat. No#: BTL-E5322Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 4.35ng/L
Detection range: 10-2000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Gap Junction Beta-6 Protein
SKU#.: ? BTL- |
E5322Hu |
Assay type |
Sandwich |
Size |
4.35ng/L |
Detection range |
10-2000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
One gap junction consists of a cluster of closely packed pairs of transmembrane Channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
UniProt accession |
30,387 |
GeneID |
10804 |
Synonyms |
Connexin-30;Cx30;Gap junction beta-6 protein;GJB 6;GJB6 |
Gene names |
GJB6 |
Research area |
Others |
Target protein |
GJB6 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5322Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.132 | 2.162 | 2.116 |
| 2.192 | |||
| 600ng/L | 1.733 | 1.789 | 1.743 |
| 1.845 | |||
| 300ng/L | 1.146 | 1.207 | 1.162 |
| 1.269 | |||
| 150ng/L | 0.62 | 0.654 | 0.608 |
| 0.689 | |||
| 75ng/L | 0.442 | 0.417 | 0.371 |
| 0.392 | |||
| 0ng/L | 0.034 | 0.046 | 0 |
| 0.058 |
Precision
We measured random samples of E5322Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 211.9 | 12.04 | 5.7 |
| Intra-Assay | 2 | 18 | 542.4 | 34.3 | 6.3 |
| Intra-Assay | 3 | 18 | 139.3 | 7.36 | 5.3 |