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Human Gap Junction Alpha-4 Protein, GJA4 ELISA Kit
Product#: BTL-E5321Hu
$686.40
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Human Gap Junction Alpha-4 Protein, GJA4 ELISA Kit
Cat. No#: BTL-E5321Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 9.61ng/L
Detection range: 20-4500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Gap Junction Alpha-4 Protein
SKU#.: ? BTL- |
E5321Hu |
Assay type |
Sandwich |
Size |
9.61ng/L |
Detection range |
20-4500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
One gap junction consists of a cluster of closely packed pairs of transmembrane Channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
UniProt accession |
37,414 |
GeneID |
2701 |
Synonyms |
Connexin-37;Cx37;Gap junction alpha-4 protein;GJA 4;GJA4 |
Gene names |
GJA4 |
Research area |
Signal Transduction |
Target protein |
GJA4 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5321Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.202 | 2.259 | 2.207 |
| 2.315 | |||
| 1200ng/L | 1.247 | 1.272 | 1.22 |
| 1.298 | |||
| 600ng/L | 0.836 | 0.829 | 0.777 |
| 0.821 | |||
| 300ng/L | 0.44 | 0.441 | 0.389 |
| 0.441 | |||
| 150ng/L | 0.304 | 0.275 | 0.222 |
| 0.246 | |||
| 0ng/L | 0.052 | 0.052 | 0 |
| 0.052 |
Precision
We measured random samples of E5321Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 809.8 | 49.93 | 6.2 |
| Intra-Assay | 2 | 18 | 1689.2 | 37.27 | 2.2 |
| Intra-Assay | 3 | 18 | 317.6 | 14.81 | 4.7 |