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Human Dynein Heavy Chain 5, Axonemal, DNAH5 ELISA Kit
Product#: BTL-E3543Hu
$686.40
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Human Dynein Heavy Chain 5, Axonemal, DNAH5 ELISA Kit
Cat. No#: BTL-E3543Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.027ng/ml
Detection range: 0.05-30ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Dynein Heavy Chain 5,Axonemal
SKU#.: ? BTL- |
E3543Hu |
Assay type |
Sandwich |
Size |
0.027ng/ml |
Detection range |
0.05-30ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles. |
UniProt accession |
529,021 |
GeneID |
1767 |
Synonyms |
Axonemal beta dynein heavy chain 5;Ciliary dynein heavy chain 5;DNAH 5;DNAH5;Dynein heavy chain 5, axonemal |
Gene names |
DNAH5 |
Research area |
Signal Transduction |
Target protein |
DNAH5 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3543Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 16ng/ml | 1.926 | 1.834 | 1.787 |
| 1.743 | |||
| 8ng/ml | 1.102 | 1.016 | 0.969 |
| 0.93 | |||
| 4ng/ml | 0.462 | 0.478 | 0.43 |
| 0.493 | |||
| 2ng/ml | 0.297 | 0.22 | 0.173 |
| 0.143 | |||
| 1ng/ml | 0.099 | 0.105 | 0.057 |
| 0.11 | |||
| 0ng/ml | 0.06 | 0.047 | 0 |
| 0.034 |
Precision
We measured random samples of E3543Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 14.3 | 0.44 | 3.1 |
| Intra-Assay | 2 | 18 | 4.9 | 0.17 | 3.4 |
| Intra-Assay | 3 | 18 | 9.5 | 0.44 | 4.6 |
