.jpg "/Content/Upload/Product/Human_ELISA_kits_Diagnocine_1200(21755).jpg")
Human Dynein Heavy Chain 17, Axonemal, DNAH17 ELISA Kit
Product#: BTL-E5207Hu
$686.40
Availability:
Ships in 24 hours
Ready to use
Human Dynein Heavy Chain 17, Axonemal, DNAH17 ELISA Kit
Cat. No#: BTL-E5207Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.046ng/mL
Detection range: 0.1-40ng/mL
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Dynein Heavy Chain 17, Axonemal
SKU#.: ? BTL- |
E5207Hu |
Assay type |
Sandwich |
Size |
0.046ng/mL |
Detection range |
0.1-40ng/mL |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Force generating protein component of the outer dynein arms (ODAs) in the sperm flagellum. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP (Probable). Plays a major role in sperm motility, implicated in sperm flagellar assembly and beating (PubMed: 31178125). |
UniProt accession |
509,313 |
GeneID |
8632 |
Synonyms |
Axonemal beta dynein heavy chain 17;Axonemal dynein heavy chain-like protein 1;Ciliary dynein heavy chain 17;Ciliary dynein heavy chain-like protein 1;DNAH 17;DNAH17;Dynein heavy chain 17, axonemal;Dynein light chain 2, axonemal |
Gene names |
DNAH17 |
Research area |
Others |
Target protein |
DNAH17 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5207Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.161 | 2.222 | 2.173 |
| 2.282 | |||
| 12ng/mL | 1.335 | 1.289 | 1.24 |
| 1.243 | |||
| 6ng/mL | 0.82 | 0.79 | 0.741 |
| 0.76 | |||
| 3ng/mL | 0.557 | 0.538 | 0.489 |
| 0.519 | |||
| 1.5ng/mL | 0.304 | 0.317 | 0.268 |
| 0.33 | |||
| 0ng/mL | 0.04 | 0.049 | 0 |
| 0.058 |
Precision
We measured random samples of E5207Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 14 | 0.46 | 3.3 |
| Intra-Assay | 2 | 18 | 7.4 | 0.54 | 7.3 |
| Intra-Assay | 3 | 18 | 4.9 | 0.36 | 7.4 |