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Human Dynein Heavy Chain 12, Axonemal, DNAH12 ELISA Kit
Product#: BTL-E5206Hu
$686.40
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Human Dynein Heavy Chain 12, Axonemal, DNAH12 ELISA Kit
Cat. No#: BTL-E5206Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 3.11ng/L
Detection range: 7-1500ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Dynein Heavy Chain 12, Axonemal
SKU#.: ? BTL- |
E5206Hu |
Assay type |
Sandwich |
Size |
3.11ng/L |
Detection range |
7-1500ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly (By similarity). |
UniProt accession |
356,942 |
GeneID |
201625 |
Synonyms |
Axonemal beta dynein heavy chain 12;Axonemal dynein heavy chain 12-like protein;Axonemal dynein heavy chain 7-like protein;Ciliary dynein heavy chain 12;DNAH 12;DNAH12;Dynein heavy chain 12, axonemal;Dynein heavy chain 7-like, axonemal;Dynein heavy chain domain-containing protein 2; |
Gene names |
DNAH12 |
Research area |
Others |
Target protein |
DNAH12 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.026 | 1.976 | 1.92 |
| 1.926 | |||
| 400ng/L | 0.969 | 1.027 | 0.971 |
| 1.085 | |||
| 200ng/L | 0.555 | 0.544 | 0.488 |
| 0.534 | |||
| 100ng/L | 0.178 | 0.184 | 0.128 |
| 0.19 | |||
| 50ng/L | 0.105 | 0.124 | 0.068 |
| 0.142 | |||
| 0ng/L | 0.056 | 0.056 | 0 |
| 0.056 |
Precision
We measured random samples of E5206Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 255.9 | 12.61 | 4.9 |
| Intra-Assay | 2 | 18 | 482.4 | 13.82 | 2.9 |
| Intra-Assay | 3 | 18 | 450.7 | 19.91 | 4.4 |