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Human Dolichyl-diphosphooligosaccharide—Protein Glycosyltransferase Subunit 1 (RPN1)
Product#: JNB-JL17700
$614.40
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DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Human Dolichyl-diphosphooligosaccharide?Protein Glycosyltransferase Subunit 1 (RPN1)
Cat.No.: JNB-JL17700
Size: 96T
Species: Human
Assay Type: Sandwich
Sensitivity: 0.8ng/mL
Detection Range: 1.56-100ng/mL
Alternative Names: RPNI; OST1; RBPH1; Ribophorin I; Dolichyl-diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit；Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1；Ribophorin-1；RPN1
Research Areas: Signal Transduction
Assay Time: 2h45m
Abbreviation: RPN1
Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RPN1. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Human RPN1 in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Human RPN1 in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human RPN1 in samples. No significant cross-reactivity or interference between Human RPN1 and analogues was observed.
Recovery rate: 80%-120%
Precision: Intra-CV and Inter-CV <10%
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