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Human DNA Repair Protein Rad51 Homolog 1, RAD51 ELISA Kit
Product#: BTL-E1370Hu
$686.40
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Human DNA Repair Protein Rad51 Homolog 1, RAD51 ELISA Kit
Cat. No#: BTL-E1370Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.11ng/ml
Detection range: 0.2-70ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Dna Repair Protein Rad51 Homolog 1
SKU#.: ? BTL- |
E1370Hu |
Assay type |
Sandwich |
Size |
0.11ng/ml |
Detection range |
0.2-70ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed: 28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed: 26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed: 26253028). |
UniProt accession |
36,966 |
GeneID |
5888 |
Synonyms |
DNA repair protein RAD51 homolog 1;hRAD51;HsRAD51;RAD51;RAD51 homolog A |
Gene names |
RAD51 |
Research area |
Others |
Target protein |
RAD51 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1370Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.546 | 2.538 | 2.482 |
| 2.531 | |||
| 20ng/ml | 1.467 | 1.48 | 1.423 |
| 1.492 | |||
| 10ng/ml | 0.904 | 0.859 | 0.802 |
| 0.814 | |||
| 5ng/ml | 0.386 | 0.419 | 0.363 |
| 0.453 | |||
| 2.5ng/ml | 0.265 | 0.23 | 0.173 |
| 0.195 | |||
| 0ng/ml | 0.057 | 0.057 | 0 |
| 0.056 |
Precision
We measured random samples of E1370Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 17.1 | 0.5 | 2.9 |
| Intra-Assay | 2 | 18 | 10.1 | 0.46 | 4.6 |
| Intra-Assay | 3 | 18 | 7.6 | 0.42 | 5.5 |