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Human Cytoskeleton-associated Protein 4, CKAP4 ELISA Kit
Product#: BTL-E4664Hu
$686.40
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Human Cytoskeleton-associated Protein 4, CKAP4 ELISA Kit
Cat. No#: BTL-E4664Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 6.88ng/L
Detection range: 15-3000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Cytoskeleton-Associated Protein 4
SKU#.: ? BTL- |
E4664Hu |
Assay type |
Sandwich |
Size |
6.88ng/L |
Detection range |
15-3000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Mediates the anchoring of the endoplasmic reticulum to microtubules. High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells. |
UniProt accession |
66,022 |
GeneID |
10970 |
Synonyms |
63-kDa cytoskeleton-linking membrane protein;CKAP 4;CKAP4;Climp-63;Cytoskeleton-associated protein 4;p63 |
Gene names |
CKAP4 |
Research area |
Cancer |
Target protein |
CKAP4 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4664Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.248 | 2.195 | 2.142 |
| 2.142 | |||
| 800ng/L | 1.456 | 1.428 | 1.375 |
| 1.4 | |||
| 400ng/L | 0.788 | 0.756 | 0.703 |
| 0.723 | |||
| 200ng/L | 0.437 | 0.438 | 0.385 |
| 0.44 | |||
| 100ng/L | 0.292 | 0.255 | 0.202 |
| 0.219 | |||
| 0ng/L | 0.046 | 0.053 | 0 |
| 0.061 |
Precision
We measured random samples of E4664Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1140.7 | 53.39 | 4.7 |
| Intra-Assay | 2 | 18 | 292.2 | 15.34 | 5.3 |
| Intra-Assay | 3 | 18 | 381.8 | 19.71 | 5.2 |