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Human Cytoplasmic Dynein 1 Heavy Chain 1, DYNC1H1 ELISA Kit
Product#: BTL-E5166Hu
$686.40
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Human Cytoplasmic Dynein 1 Heavy Chain 1, DYNC1H1 ELISA Kit
Cat. No#: BTL-E5166Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 4.66ng/L
Detection range: 10-2000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Cytoplasmic Dynein 1 Heavy Chain 1
SKU#.: ? BTL- |
E5166Hu |
Assay type |
Sandwich |
Size |
4.66ng/L |
Detection range |
10-2000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression (PubMed: 27462074). |
UniProt accession |
532,408 |
GeneID |
1778 |
Synonyms |
Cytoplasmic dynein 1 heavy chain 1;Cytoplasmic dynein heavy chain 1;DYNC 1H1;DYNC1H1;Dynein heavy chain, cytosolic |
Gene names |
DYNC1H1 |
Research area |
Others |
Target protein |
DYNC1H1 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5166Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.299 | 2.303 | 2.275 |
| 2.307 | |||
| 600ng/L | 1.807 | 1.769 | 1.741 |
| 1.731 | |||
| 300ng/L | 1.12 | 1.142 | 1.114 |
| 1.165 | |||
| 150ng/L | 0.682 | 0.676 | 0.648 |
| 0.67 | |||
| 75ng/L | 0.398 | 0.419 | 0.39 |
| 0.439 | |||
| 0ng/L | 0.029 | 0.028 | 0 |
| 0.028 |
Precision
We measured random samples of E5166Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 80.7 | 3.4 | 4.2 |
| Intra-Assay | 2 | 18 | 583.2 | 34.65 | 5.9 |
| Intra-Assay | 3 | 18 | 165 | 10.18 | 6.2 |