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Human C-C Motif Chemokine 5, CCL5/RANTES ELISA Kit
Product#: BTL-E5009Hu
$686.40
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Human C-C Motif Chemokine 5, CCL5/RANTES ELISA Kit
Cat. No#: BTL-E5009Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 5.26ng/L
Detection range: 10-2000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
C-C Motif Chemokine 5
SKU#.: ? BTL- |
E5009Hu |
Assay type |
Sandwich |
Size |
5.26ng/L |
Detection range |
10-2000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed: 16791620, PubMed: 1380064, PubMed: 8525373, PubMed: 9516414, PubMed: 15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed: 23979485). |
UniProt accession |
9,990 |
GeneID |
6352 |
Synonyms |
C-C motif chemokine 5;CCL 5;CCL5;EoCP;Eosinophil chemotactic cytokine;RANTES(3-68;RANTES(4-68;SIS-delta;Small-inducible cytokine A5;T cell-specific protein P228;T-cell-specific protein RANTES;TCP228 |
Gene names |
CCL5 |
Research area |
Immunology; Cardiovascular |
Target protein |
CCL5 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5009Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.347 | 2.504 | 2.445 |
| 2.66 | |||
| 600ng/L | 1.594 | 1.489 | 1.431 |
| 1.385 | |||
| 300ng/L | 0.75 | 0.748 | 0.689 |
| 0.746 | |||
| 150ng/L | 0.38 | 0.452 | 0.394 |
| 0.525 | |||
| 75ng/L | 0.275 | 0.257 | 0.199 |
| 0.24 | |||
| 0ng/L | 0.066 | 0.059 | 0 |
| 0.052 |
Precision
We measured random samples of E5009Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 216.8 | 9.11 | 4.2 |
| Intra-Assay | 2 | 18 | 632 | 15.77 | 2.5 |
| Intra-Assay | 3 | 18 | 463.5 | 19.93 | 4.3 |