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Human Apelin Receptor, APLNR ELISA Kit
Product#: BTL-E1754Hu
$686.40
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Human Apelin Receptor, APLNR ELISA Kit
Cat. No#: BTL-E1754Hu
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 7.37ng/L
Detection range: 15-3000ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Human
Product overview
Full product name
Apelin Receptor
SKU#.: ? BTL- |
E1754Hu |
Assay type |
Sandwich |
Size |
7.37ng/L |
Detection range |
15-3000ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Human |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed: 11090199, PubMed: 25639753, PubMed: 28137936). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (PubMed: 25639753, PubMed: 28137936). (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia (PubMed: 11090199). |
UniProt accession |
42,660 |
GeneID |
187 |
Synonyms |
Angiotensin receptor-like 1;Apelin receptor;APLNR;G-protein coupled receptor APJ;G-protein coupled receptor HG11 |
Gene names |
APLNR |
Research area |
Neuroscience |
Target protein |
APLNR |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1754Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.096 | 2.215 | 2.191 |
| 2.335 | |||
| 800ng/L | 1.428 | 1.318 | 1.294 |
| 1.209 | |||
| 400ng/L | 0.574 | 0.621 | 0.597 |
| 0.669 | |||
| 200ng/L | 0.331 | 0.317 | 0.293 |
| 0.304 | |||
| 100ng/L | 0.146 | 0.162 | 0.138 |
| 0.178 | |||
| 0ng/L | 0.022 | 0.024 | 0 |
| 0.026 |
Precision
We measured random samples of E1754Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 553.5 | 26.08 | 4.7 |
| Intra-Assay | 2 | 18 | 1138.9 | 42.96 | 3.8 |
| Intra-Assay | 3 | 18 | 288.2 | 13.85 | 4.8 |