Hepatitis B virus Core antigen (Recombinant)

Cat. No.: FNK-BCL-HBC-01

Size: 250μl

Storage: -20℃ (1 year)


Introduction:

HBsAg (also called Bionanocapsule) is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials, and developed the production process of various HBsAgs . 



Specifications:

Source: E.coli

Appearance: Lyophilized white powder

Structure: Particle (about 40 nm by dynamic light scattering method) composed of 21kDa C protein.

Dissolve: For 100 µg vial, added 500 µL of water to the vial that makes a antigen solution at 0.2 mg/mL in PBS (137 mM NaCl, 8.1 mM Na₂HPO4?12H₂O, 2.68 mM KCl, 1.47 mM KH₂PO₄, pH 7.2 - 7.4) containing 1% sucrose.

Purity: over 95%

Endotoxin : < 50 EU/mg protein

Usage: Standard antigen for ELISA, western, and others Tagged Pre-S1 peptides are better for ELISA plate immobilization.

*Caution :The antigen may be adsorbed on plastic tubes especially low concentration (below 100ng/mL). Please use protein low bind tubes at lower concentration.


SDS-PAGE (silver staining)


HbcAg of 100ng was subjected to SDS-PAGE (M :Marker)