FluxMPS™ Ham's F-12K (Kaighn's) Medium: 1X Liquid

FluxMPS™ Ham's F-12K (Kaighn's) Medium: 1X Liquid

FluxMPS™ DCP-H12K1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Ham's F-12K (Kaighn's) medium engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. Enriched with putrescine, thymidine, hypoxanthine, and elevated sodium pyruvate for primary hepatocyte culture, the formulation is processed through Diagnocine's four-stage filtration train, reducing particulate load below conventional 0.22 µm–filtered media. Formulation: [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (1.260 g/L), [+] Sodium Pyruvate.

• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final polish for microfluidic-channel compatibility
• Complete Ham's F-12K (Kaighn's) formulation: 1.260 g/L glucose, 292 mg/L L-glutamine, 220 mg/L sodium pyruvate, 2500 mg/L sodium bicarbonate, and phenol red pH indicator
• Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85>), tested per manufacturing batch
• Enriched with putrescine, thymidine, hypoxanthine, and zinc for primary hepatocyte and liver-cell culture per the Kaighn's modification
• Manufactured under an ISO 13485:2016 quality management system with full lot traceability; final QC at Diagnocine, Totowa, NJ
• 48 formulation components verified per lot across inorganic salts, amino acids, vitamins, and metabolic additives
• Custom pH, glucose concentration, salts, and nutrient adjustments available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and aggregates that clog microfluidic channels and interfere with sensor readings. FluxMPS™ reduces these failure modes with a four-stage filtration train reaching a 0.04 µm final cut-off.

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish, delivering purity levels not achievable with standard 0.22 µm filtration.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter, including the mycoplasma size range (0.2–0.3 µm).

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge downstream.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) environment.

Designed for next-generation cell models

FluxMPS™ DCP-H12K1X supports demanding platforms from single-channel microfluidic chips to hepatocyte-focused multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk for solenoid valves and inline optical sensors
• Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

48 components across 4 formulation categories, organized into 3 tabs below, verified per lot with CAS numbers for raw-material traceability. Ham's F-12K (Kaighn's) modification carries elevated zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-H12K1X compares

FluxMPS™ DCP-H12K1X vs. conventional 0.22 µm–filtered Ham's F-12K (Kaighn's) formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-H12K1X.

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and hepatocyte culture research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Bell CC, et al. Characterization of primary human hepatocyte spheroids for repeat-dose toxicity studies. Sci Rep. 2016;6:25187. doi:10.1038/srep25187
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j