FluxMPS™ Ham's F-12 Nutrient Mixture w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ Ham's F-12 Nutrient Mixture w/o L-Glutamine, Sodium Bicarbonate, Phenol Red: 1X Liquid

FluxMPS™ DCP-H12-QBR1X is a Microfluidics Suitable, ultra-filtered Ham's F-12 Nutrient Mixture engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. This formulation excludes L-Glutamine, Sodium Bicarbonate, and Phenol Red while retaining glucose, sodium pyruvate, calcium, and magnesium.

• Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
• Formulation: [+] Calcium, [+] Magnesium, [+] Glucose (1801.60 mg/L), [+] Sodium Pyruvate (110.100 mg/L) | [-] L-Glutamine, [-] Sodium Bicarbonate, [-] Phenol Red
• CO₂-independent formulation (bicarbonate-free); use HEPES or another organic buffer for pH control
• Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
• Sterility confirmed by USP <71> 14-day no-growth incubation
• 44 verified ingredients across inorganic salts, amino acids, vitamins, and other components
• Custom pH, salt, and nutrient adjustments available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels and corrupt sensor signals. FluxMPS™ targets these failure modes with a four-stage sub-0.04 µm filtration train.

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reach a final 0.04 µm polish under ISO Class 5 aseptic conditions — engineered for microchannel-safe purity beyond conventional 0.22 µm–filtered media.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge downstream.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains mycoplasma-scale organisms (0.2–0.3 µm) and sub-micron particulates that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge and providing full pair-wise redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter ahead of aseptic fill & finish in a validated ISO Class 5 (Class 100) environment.

Designed for next-generation cell models

FluxMPS™ DCP-H12-QBR1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers — a distinct, higher tier above the Microfluidics Suitable product on this page.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
• Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

44 ingredients verified per lot with CAS numbers for raw-material traceability. This Ham's F-12 base includes zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-H12-QBR1X compares

FluxMPS™ DCP-H12-QBR1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-H12-QBR1X.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j