HRPQuench-PBS

Cat No.: DCP-HRPPBS1X

Size: 100 mL

Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.

HRPQuench-PBS represents a significant advancement in horseradish peroxidase (HRP) signal management for immunoassays, providing precise control during sequential and multiplex immunostaining protocols. This innovative phosphate-buffered saline-based solution, formulated with a proprietary blend of enzyme inhibitors and stabilizers, ensures rapid and complete inactivation of HRP without affecting tissue integrity or subsequent antibody binding. By effectively eliminating cross-reactivity and false-positive signals, HRPQuench-PBS markedly enhances the specificity and reliability of complex immunohistochemistry and immunofluorescence procedures. Its optimized formulation is particularly valuable in workflows involving multiple HRP-conjugated antibodies, allowing for clear, distinct labeling of different antigens within the same sample. In addition to improving staining quality, HRPQuench-PBS streamlines procedures and reduces overall experiment time and reagent consumption, making it an essential tool for advanced immunological research.

Applications

• Enhanced Antigen-Antibody Interactions: Ideal for immunoassays, Western blotting, and ELISA, where improved binding efficiency is crucial.
• Research and Diagnostic Tools: Suitable for research settings to enhance assay sensitivity and specificity, and in diagnostic settings for precise antigen or antibody detection.

Advantages:

• Enhanced Binding Efficiency: The combination of PEG 6000, PVP-40, and BSA helps reduce non-specific binding, enhancing the specificity and sensitivity of antigen-antibody interactions.
• Cell and Tissue Compatibility: The isotonic nature of PBS ensures that cells and tissues remain viable during experimental procedures, making it suitable for cell culture and tissue handling applications.

Composition:
 

Quality Control:

• Appearance: Clear, Colorless Liquid.

• pH: 7.4

• Sterility: Non-Sterile

Storage and Shelf Life:

• Storage: 4℃ 

• Shelf life: 1 year

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.