Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
Description:
The medium stripping buffer (low-pH glycine-HCl, 0.1 M, pH 2.2) removes primary and secondary antibodies from western blot membranes or other immunocomplex assays. It can dissociate the antibody-antigen interactions via a low-pH glycine solution. It alters the antibody's binding site to free it from bound antigens. The membrane can be re-probed with other antibodies, which enables scientists to detect multiple proteins of interest on the same membrane. It provides minimal disruptions to the blotted proteins. It contains 0.1% SDS to help antibody-antigen dissociation. Low pH and SDS detergents work together to alter the structure of the antibodies bound to the membrane, rendering their antigen-binding sites inactive, and glycine will protect protein integrity.
This glycine-HCl stripping buffer is considered a "mild" method compared to harsher stripping buffers containing high concentrations of SDS and reducing agents at higher temperatures. It allows for efficient antibody removal while potentially preserving the antibodies for re-use.
*The buffer volume must be enough to move side-to-side (i.e., wave motion) inside the container when placed on a rocking platform (Rockers); we do not recommend rotators (circular motion shakers).
Advantages:
The Medium Stripping Buffer enables the membrane to be re-probed with new antibodies.
It saves time, samples, and reagents as researchers don’t have to repeat the Western blot process repeatedly.
It optimizes experimental conditions.
It can correct errors, such as if the wrong antibody is used.
DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: Room temperature
Shelf life: 1 year
CUSTOMIZATION:Please INQUIRE if other concentrations, detergents, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration two times. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
