Glyceraldehyde-3-Phosphate Dehydrogenase YK1, Thermostable
Glyceraldehyde-3-Phosphate Dehydrogenase YK1, Thermostable
Cat. No. FNK-GAP-97-01
Stroage -20°C
Size 1ml
EC No. 1.2.1.12
Substrate glyceraldehyde-3-phosphate
Reaction product 1,3-bisphosphoglyceric acid
Reaction temperature ~70℃
Reaction pH 8.0~11.0
Lot No. 1-H201A
Protein conc. 11 mg/ml
Form 20 mM Tris-HCl (pH 7.5), 20 mM KCl
Activity 120 U/ml
Notes For research use only
Description
Thermostable Enzymes Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".
Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.
Features of Thermostable Enzyme
- Highly stable and durable for long term use
- High reactivity at high temperature (70 - 100°C)
- Resistance to organic solvent is expected
- Special instruments to inhibit bacterial growth are not required.
- Reaction speficity is much higher compared with chemical reaction
- Speed of reaction can be accelerated
Activity measurement
Reaction mix (20 mM Potassium phosphate buffer (pH 7.0), 1 mM Glyceraldehyde 3-phosphate, 1 mM NAD+ and appropriate amount of the enzyme) was incubated at 50 oC and A340 was monitored. One unit is defined as the amount of enzyme producing 1 ?mol of NADH (?340=6.22 mM-1 cm-1 ) per 1 minute using Glyceraldehyde 3-phosphate as a substrate.
