FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose w/o Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-GMEM-BJN1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Glasgow's Minimum Essential Medium (GMEM) engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a validated four-stage filtration train (0.1 µm ×2 + 0.04 µm ×2), it is high-glucose (4500 mg/L) and supplied without sodium bicarbonate buffer for flexible use across incubation systems.

• High glucose Glasgow's Minimum Essential Medium (4500 mg/L) formulated without sodium bicarbonate buffer
• Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a validated 0.04 µm final polish
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> Bacterial Endotoxins Test)
• L-Glutamine included at 292 mg/L; phenol red included at 15 mg/L for visual pH reference
• Sodium bicarbonate excluded — buffer system to be added by the user per their CO₂ incubation protocol
• 29 verified ingredients per lot across inorganic salts, amino acids, vitamins and other components, with CAS traceability
• Manufactured under an ISO 13485:2016 quality management system with full lot traceability
• Custom pH, glucose, salt and buffer modifications available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and cellular debris that confound sensitive assays and foul microfluidic channels.

Quadruple-stage filtration system

Four serial filtration stages — two 0.1 µm prefilters each paired with a dedicated 0.04 µm final filter — reaching a validated 0.04 µm final polish under ISO Class 5 aseptic fill conditions, purpose-built for microfluidic channels and organ-on-a-chip (OoC) culture systems.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates, including mycoplasma-sized organisms (0.2–0.3 µm), that pass a standard 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge downstream.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; ISO Class 5 aseptic fill & finish. Final product QC release gate.

OoC and MPS Applications

FluxMPS™ DCP-GMEM-BJN1X delivers Microfluidics Suitable, ultra-filtered purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactors and robotic perfusion systems requiring the ultra nano-filtered tier.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
• Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion and recirculation systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Full composition (mg/L)

Glasgow's Minimum Essential Medium (GMEM), High Glucose w/o Sodium Bicarbonate: 1X Liquid: 29 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-GMEM-BJN1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

How DCP-GMEM-BJN1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEM-BJN1X and its use in OoC and MPS applications.

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

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3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
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8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309