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FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth(TPB), 25mM HEPES: 1X Liquid

Product#: DCP-GMEMH-N1X
$49.50
DCP-GMEMH-N1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth(TPB), 25mM HEPES: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains Glucose (High, 4500 mg/L)

FluxMPS™ DCP-GMEMH-N1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid GMEM formulation supplemented with Tryptose Phosphate Broth (TPB) and 25 mM HEPES, engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final polish removes the sub-micron particulates and microaggregates that a conventional 0.22 µm filter passes through.

  • High-glucose GMEM (4500 mg/L D-glucose) enriched with 25 mM HEPES and Tryptose Phosphate Broth for fastidious and demanding cell lines
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore-size polish
  • Batch release specification: endotoxin < 0.05 EU/mL (LAL, USP <85>)
  • Sodium bicarbonate (2750 mg/L) plus 25 mM HEPES co-buffering; approximately 7.2% CO2 required to hold pH 7.4
  • Manufactured under an ISO 13485:2016 quality management system with full lot traceability
  • 32 verified components with CAS traceability across inorganic salts, amino acids, vitamins and other additives
  • Phenol red sodium salt (15 mg/L) included as a visual pH indicator — red-colored, clear solution
  • Custom pH, glucose, HEPES and nutrient modifications available on request
CAT. NO.
DCP-GMEMH-N1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth, 25mM HEPES: 1X Liquid
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose (High)
  • Glucose4500.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
  • Sizes500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particles, subvisible particulates, and microaggregates that confound sensitive on-chip assays and foul microfluidic channels.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Formulated for particle-sensitive OoC microfluidic chips.

target

Total metabolic control

High-glucose GMEM base with defined L-glutamine, Tryptose Phosphate Broth and HEPES levels for precise control of carbon source and buffering in your experimental system.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm resistivity (ASTM D1193 / ISO 3696). Low trace-metal and organic carbon (TOC) content minimizes uncontrolled variables in sensitive downstream assays.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy, biosensor measurement and live-cell imaging on-chip. Phenol red is present in this formulation for visual pH indication.

science

Rich, stable nutrient profile

32 verified ingredients per lot across inorganic salts, amino acids, vitamins, and other additives including Tryptose Phosphate Broth. Full CAS traceability, micro-batch precision manufacturing.

tune

Customization on demand

pH, nutrient concentrations, HEPES level, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic fill conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris and protein precipitates; protects the downstream 0.04 µm final filter and extends its service life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates below the resolution of standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive grade prefilter, protecting the second 0.04 µm cartridge from bioburden and particulate loading.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate 0.04 µm polishing filter; aseptic fill & finish in an ISO Class 5 (Class 100) environment. Final product QC release gate.

Performance vs. conventional media

FluxMPS™ DCP-GMEMH-N1X runs a repeated prefilter + final-filter pair, twice in series, reaching a 0.04 µm final cut-off with mycoplasma-retentive prefiltration at every production stage.

4
Serial filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-GMEMH-N1X Glasgow's Minimum Essential Medium GMEM High Glucose with Tryptose Phosphate Broth 25mM HEPES Quadruple-stage filtration for organ-on-a-chip and microfluidic cell culture, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-GMEMH-N1X.
© Diagnocine® — DCP-GMEMH-N1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-GMEMH-N1X delivers ultra-filtered, Microfluidics Suitable purity for organ-on-a-chip, microphysiological system and microfluidic applications requiring GMEM with Tryptose Phosphate Broth supplementation.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates that foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered GMEM/TPB/HEPES formulation for organ-on-a-chip, tissue-on-a-chip and body-on-a-chip devices where particle-free media prevents channel fouling.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose formulation supports Warburg-effect metabolic studies and glycolysis-dependent cancer cell line culture with precise carbon-source control.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-clean base with Tryptose Phosphate Broth enrichment for iPSC differentiation protocols requiring defined, particulate-free media.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free, TPB-enriched formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for 13C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate background for confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose (High, 4500 mg/L); also contains Tryptose Phosphate Broth
Appearance Red-colored, clear solution
Total ingredients 32
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Phenol Red 15.000 mg/L (phenol red sodium salt)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 7.2% CO2 (calculated from 2750 mg/L NaHCO3 plus 25 mM HEPES co-buffering, target pH 7.4; validate per cell line and incubator set-point)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth, 25mM HEPES: 1X Liquid — 32 ingredients verified per lot with CAS numbers for raw-material traceability where known.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 10421-48-4 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2750.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 42.000
L-Cystine 30925-07-6 24.000
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride 5934-29-2 21.000
L-Isoleucine 73-32-5 52.400
L-Leucine 61-90-5 52.400
L-Lysine hydrochloride 657-27-2 73.100
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 33.000
L-Threonine 72-19-5 47.600
L-Tryptophan 73-22-3 8.000
L-Tyrosine Disodium salt 69847-45-6 52.000
L-Valine 72-18-4 46.800
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxal hydrochloride 65-22-5 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
i-Inositol 87-89-8 3.600
D-Glucose 50-99-7 4500.000
HEPES buffer 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 15.000
Tryptose Phosphate Broth   2950.000
Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-GMEMH-N1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity (ASTM D1193 / ISO 3696 Type 1). Low trace-metal and TOC content controlled at the water-purification stage.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in an ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to limit recontamination risk.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Each batch tested independently, not pooled or blended across lots.

Endotoxin USP <85> BET

LAL-based endotoxin test per manufacturing batch. Release specification: < 0.05 EU/mL — well below the levels reported to activate TLR4-mediated responses in sensitive immune cell assays.

Particulate USP <788> Method 1

Light obscuration particulate count. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm per batch.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-GMEMH-N1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-GMEMH-N1X (FluxMPS™) Conventional 0.22 µm Filtered Standard Alternative
Grade Microfluidics Suitable Standard grade Standard grade
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose (High) Standard GMEM Standard GMEM
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Type 2 typical Type 2 typical
Manufacturing QMS ISO 13485:2016 ISO 9001 typical Variable
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEMH-N1X and its use in OoC and MPS applications.

Yes. DCP-GMEMH-N1X is a Microfluidics Suitable formulation engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces the risk of microfluidic channel fouling, making it suitable for OoC, ToC, BoC and LoC applications sensitive to particulate contamination.
FluxMPS uses a Quadruple-stage system (0.1 µm ×2 + 0.04 µm ×2, four passes), running two dedicated prefilter + final-filter pairs in series, reaching a 0.04 µm final cut-off and mycoplasma-retentive prefiltration that standard 0.22 µm filtration does not provide.
Tryptose Phosphate Broth (2950 mg/L) supplies additional peptides and cofactors for fastidious or difficult-to-culture cell lines, and 25 mM HEPES provides supplemental buffering alongside the 2750 mg/L sodium bicarbonate already in the formulation. This is a basal formulation; further supplementation (serum, growth factors, or defined additives) should be selected based on your specific cell type and protocol.
Yes. This formulation is buffered with sodium bicarbonate (2750 mg/L) plus 25 mM HEPES. Based on the bicarbonate concentration, approximately 7.2% CO2 is required to maintain the target pH of 7.4 in a standard incubator; validate the exact set-point for your specific incubator and cell line.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements (e.g., B27, N2), growth factors, or other additives as required by your cell type and protocol. Pre-filter serum and other protein-containing supplements through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for serum, as it will retain lipoproteins and clog rapidly.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by the Limulus Amebocyte Lysate (LAL) assay per USP <85> (Bacterial Endotoxins Test), with an assay sensitivity of 0.005 EU/mL. Every batch is tested before release; a Certificate of Analysis is available on request.
Yes. CoA is available per batch upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, particulate (USP <788> Method 1), appearance, and full raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC/MPS applications and FluxMPS™ ultra-filtered cell culture media.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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