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FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose: 1X Liquid

Product#: DCP-GMEM-JN1X
$44.00
DCP-GMEM-JN1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains High Glucose

FluxMPS™ DCP-GMEM-JN1X is a Microfluidics Suitable, ultra-filtered high-glucose GMEM formulation engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High-glucose GMEM formulation (4,500 mg/L D-Glucose) with 292 mg/L L-Glutamine and 2,750 mg/L sodium bicarbonate buffering
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • No growth after 14-day sterility incubation (USP <71>); mycoplasma control via 0.1 µm mycoplasma-retentive filtration stages
  • 30 verified ingredients per lot across inorganic salts, amino acids, vitamins and other components, with CAS traceability
  • Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
  • Phenol red indicator included (15.000 mg/L) for visual pH monitoring
  • pH, glucose concentration, HEPES, salts, and nutrient composition customizable on request
CAT. NO.
DCP-GMEM-JN1X | Cell culture media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Glasgow's Minimum Essential Medium (GMEM), High Glucose: 1X Liquid
  • Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium
  • Glucose4500.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
  • Sizes Available500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma, subvisible particulates, and endotoxin fragments that confound cell assays.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-controlled media for OoC microfluidic chips.

target

Total metabolic control

Defined high-glucose formulation for precise control of carbon sources, nutrients, and metabolic inputs in your experimental system.

water_drop

Ultrapure-grade water

18.2 MΩ·cm resistivity (ASTM D1193 Type I / ISO 3696). Low trace-metal and total organic carbon (TOC) profile supports reproducible, well-controlled culture conditions.

visibility

Low background for imaging

Ultra-low particulate baseline for confocal microscopy, biosensor, and live-cell imaging applications on-chip.

science

Rich, stable nutrient profile

30 verified ingredients per lot. Full CAS traceability. Micro-batch precision manufacturing.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated 0.1 µm prefilter passes, each paired with its own 0.04 µm final filter — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions, delivering ready-to-use, ultra-filtered media for microfluidic applications.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cellular debris, and protein aggregates; provides mycoplasma-retentive filtration (0.2–0.3 µm size range, validated with A. laidlawii; not tested per lot). Protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First sub-micron polishing pass; retains particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm final filter cartridge; redundant large-particulate and mycoplasma-retentive barrier.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish; final product QC release gate.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration (0.2–0.3 µm size range); mycoplasma testing is not performed per lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-GMEM-JN1X Quadruple-stage filtration system GMEM High Glucose cell culture media for organ-on-a-chip and microfluidic applications Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-GMEM-JN1X.
© Diagnocine® — DCP-GMEM-JN1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-GMEM-JN1X delivers ultra-filtered purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant available on request for automated bioreactors and robotic perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip where particle-controlled media reduces channel fouling.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose formulation supports NCI-60 cancer lines and Warburg effect metabolic studies with defined nutrient control.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-clean base for iPSC differentiation protocols requiring defined, particulate-controlled media.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-controlled formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate background for confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium
Appearance Red-colored, clear solution (phenol red indicator)
Total ingredients 30
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Approximately 7% CO2 (derived from 2,750 mg/L NaHCO3 to maintain pH 7.4; validate empirically)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glasgow's Minimum Essential Medium (GMEM), High Glucose: 1X Liquid: 30 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2750.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 42.000
L-Cystine 30925-07-6 24.000
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride 5934-29-2 21.000
L-Isoleucine 73-32-5 52.400
L-Leucine 61-90-5 52.400
L-Lysine hydrochloride 657-27-2 73.100
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 33.000
L-Threonine 72-19-5 47.600
L-Tryptophan 73-22-3 8.000
L-Tyrosine Disodium salt 69847-45-6 52.000
L-Valine 72-18-4 46.800
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxal hydrochloride 65-22-5 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.000
i-Inositol 87-89-8 3.600
Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-GMEM-JN1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity (ASTM D1193 Type I / ISO 3696). Low trace-metal and TOC profile controlled at the manufacturing source.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to prevent recontamination.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

LAL assay per batch. Release specification: < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate analysis. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-GMEM-JN1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-GMEM-JN1X (FluxMPS™) Conventional 0.22 µm Filtered GMEM Standard GMEM Alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium Standard GMEM formulation Standard GMEM formulation
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Type 2 typical Type 2 typical
Manufacturing QMS ISO 13485:2016 ISO 9001 typical Variable
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEM-JN1X and its use in OoC and MPS applications.

Yes. DCP-GMEM-JN1X is specifically engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces microfluidic channel fouling, making it suitable for OoC, ToC, BoC, and LoC applications with sensitivity to particulate contamination.
FluxMPS uses a Quadruple-stage system (0.1 µm ×2 + 0.04 µm ×2), providing approximately 5× lower particulate counts and mycoplasma-retentive filtration that standard 0.22 µm filtration cannot provide.
This formulation contains 4,500 mg/L D-Glucose (High Glucose) along with 292 mg/L L-Glutamine and 2,750 mg/L sodium bicarbonate, matching classical high-glucose GMEM specifications for glycolytically active cell lines. If your protocol calls for low-glucose culture, do not add additional glucose (already present at 4,500 mg/L); instead request a low-glucose GMEM variant or a glucose-free basal medium for your own dilution. Contact support@diagnocine.com for a custom formulation.
Yes. This formulation contains 2,750 mg/L sodium bicarbonate as its buffering system. Based on the Henderson-Hasselbalch relationship, maintaining the stated pH of 7.4 with this bicarbonate concentration requires an atmosphere of approximately 7% CO2. Validate against your specific incubator and altitude before use.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements (e.g., B27, N2), growth factors, or other additives as required by your cell type and experimental protocol. When adding serum or other protein-containing supplements, pre-filter through a 0.2 µm low-protein-binding PES or PVDF membrane; defined, protein-free additions may be filtered at 0.1 µm.
Endotoxin is controlled per manufacturing batch to a release specification of less than 0.05 EU/mL, verified by a Limulus Amebocyte Lysate (LAL) assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) before each batch is released. A Certificate of Analysis is available on request.
Yes. CoA is available per lot upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, mycoplasma control status, USP <788> particulate data, appearance, and full raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media applications.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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