RamDA-seq™ Single Cell Kit24 rxn · TYB-RMD-101T
$1,144.80$572.40
- Full-length total RNA from 1–100 cells or 10 pg–1 ng RNA
- cDNA/dsDNA kit; library prep reagents sold separately
GenNext™ RamDA-seq™ Total RNA-seq Kits Promotion
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Product Spotlight · Single-Cell Total RNA-seq · TOYOBO
See the RNA that oligo(dT) Leaves Behind, in a Single Cell
GenNext™ RamDA-seq™ and Shin-RamDA-seq™ are described by their RIKEN developers as the first full-length total RNA-seq method for single cells (Nature Communications, 2018). No cDNA PCR step. Poly(A) and non-poly(A) RNA in one run.
One Cell Holds Only Picograms of RNA
RamDA-seq™ kits amplify cDNA during reverse transcription, using random primers. They capture RNA with or without a poly(A) tail, across its full length, without a cDNA PCR step. To mark their U.S. launch, both 24-reaction kits are 50% off list price.
How RamDA-seq™ Compares
| 10x Chromium 3′/5′ | Takara SMART-Seq mRNA Single Cell | NEBNext Single Cell / Low Input | RamDA-seq™ | Shin-RamDA-seq™ Stranded | |
|---|---|---|---|---|---|
| Input | Cell / nuclei suspension | Single cells or nuclei | Single cells, or 2 pg–200 ng RNA | 1–100 cells, or 10 pg–1 ng | 1–100 cells, or 10 pg–1 ng |
| RT priming | Poly(dT) | Oligo(dT) | Oligo(dT) | Random primers (NSR optional) | Random primers (NSR optional) |
| Coverage | 3′ or 5′ end | Full-length mRNA | Full-length mRNA | Full-length total RNA | Full-length total RNA, stranded |
| cDNA PCR amplification step | Yes | Yes | Yes | No | No |
| Instrument | Chromium instrument | Thermocycler | Thermocycler | Thermocycler | Thermocycler |
Comparison covers the kits named above, based on each manufacturer’s published product information as of September 2026.
Ultra-Low Input Sensitivity
About 5,000 More Genes from 10 pg in TOYOBO’s Test
From 10 pg of mouse ES cell total RNA, RamDA-seq™ detected about 5,000 more genes than an unnamed comparison kit run with 18 cycles of cDNA PCR.* In separate TOYOBO data, Shin-RamDA-seq™ detected over 10,000 genes from 1 ng of FFPE RNA.
*TOYOBO data. The comparison kit is not one of the kits named in the table above.
Beyond Poly(A)
Poly(A) and Non-poly(A) RNA
Random priming captures lncRNAs, pre-mRNA and histone RNA, plus circRNA with Shin-RamDA-seq™. Optional NSR primers (human or mouse, sold separately) reduce rRNA reads.
Coverage
Full-length Coverage of Long Transcripts
TOYOBO specifies coverage of target RNAs of 10 kb and longer. In TOYOBO’s data, RamDA-seq™ covered the lncRNAs Neat1 and Malat1 across their full length.
Read coverage of Neat1 and Malat1, same samples. Data: TOYOBO.
No cDNA Pre-Amplification
Amplify While You Synthesize
RT-RamDA™ uses the strand-displacement activity of reverse transcriptase to amplify cDNA as it is made, with no amplification adaptors and no cDNA PCR step. TOYOBO reports this reduces PCR amplification bias.
All-in-one kit
Stranded, Full-length Total RNA, Library Prep Included
Shin-RamDA-seq™ preserves strand information, so antisense and overlapping genes can be distinguished. It includes an rRNA remover and library prep reagents; index kit and beads are sold separately. In TOYOBO’s data from single-cell-quantity RNA, it covered the ~20 kb NEAT1_2 transcript in full.
Single-cell quantity of K562 RNA: full-length NEAT1_2 and opposite-strand genes MRRF / RBM18. Data: TOYOBO.
Research applications
Where full-length, random-primed total RNA from very little input adds the most.
- Single-cell total RNA-seq of sorted cells (1–100 cells)
- Ultra-low-input RNA-seq (10 pg–1 ng)
- Isoforms and alternative splicing
- Nuclear RNA: pre-mRNA and lncRNA
- Antisense and overlapping genes (Shin-RamDA-seq™)
- Degraded and FFPE RNA (Shin-RamDA-seq™)
- Recursive splicing and enhancer RNAs (Hayashi et al., 2018)
Already running droplet-based scRNA-seq?
Use it to find the population that matters, then sort those cells and run RamDA-seq™ to see their full transcripts in depth.
Use it to find the population that matters, then sort those cells and run RamDA-seq™ to see their full transcripts in depth.
U.S. launch offer · through December 31, 2026
Choose your kit: 50% off list price
Shin-RamDA-seq™ Single Cell Stranded Kit24 rxn · TYB-RML-101T
$1,188.00$594.00
- Stranded, full-length total RNA from 1–100 cells or 10 pg–1 ng RNA
- Library prep reagents included; index kit and beads sold separately
50% off the diagnocine.com list price on TYB-RMD-101T and TYB-RML-101T only, through December 31, 2026. [Quantity limit / eligibility terms]. Not valid on 96-reaction kits or NSR primers (TYB-NSR-101 human, TYB-NSR-102 mouse).
For Research Use Only (RUO). Not for use in diagnostic procedures.
Reference: Hayashi T. et al. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. Nat Commun 9:619 (2018).
Shin-RamDA-seq™, RamDA-seq™ and RT-RamDA™ are trademarks of RIKEN. GenNext™ is a trademark of TOYOBO CO., LTD. Chromium is a trademark of 10x Genomics, Inc. SMART-Seq is a trademark of Takara Bio Inc. NEBNext is a trademark of New England Biolabs, Inc. Diagnocine is not affiliated with 10x Genomics, Takara Bio or New England Biolabs.
Questions about the best kit for your model? Email support@diagnocine.com or call (201) 681-7263.
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