Gel Loading Solution [6X]
FluxMPS™ Gel Loading Solution [6X]
FluxMPS™ Gel Loading Solution [6X] is an ultrapure, sterile 6X loading buffer engineered for DNA and RNA electrophoresis sample preparation. Manufactured with quadruple-stage 0.1 µm / 0.04 µm membrane filtration and formulated with a defined glycerol and dual-dye system, it delivers reliable well loading and real-time migration tracking for demanding molecular biology and organ-on-a-chip (OoC) nucleic acid QC workflows.
- Positioned as one of the cleanest gel loading buffers available for cell and molecular biology experiments
- Quadruple-stage filtration: 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice
- Sterile, ultrapure formulation manufactured in an aseptic environment
- 30% glycerol content for consistent sample density and accurate well loading
- Dual tracking-dye system — bromophenol blue and xylene cyanol FF — for real-time electrophoresis monitoring
- No detectable DNase or RNase activity after extended incubation testing
- Compatible with agarose gel and non-denaturing polyacrylamide gel electrophoresis
- Custom concentrations, alternate tracking dyes, and pH modifications available on request
- Format3 x 1 mL
- Concentration6X
- AppearanceBlue-purple to violet
- Filtration0.1 µm membrane (x2) + 0.04 µm membrane (x2)
- SterilityFilter-sterilized, aseptic environment
- DNase ActivityNone detected
- RNase ActivityNone detected
- Storage-20°C
- Shelf Life1 year
- Water QualityUltrapure Type 1 (18.2 MΩ·cm)
Engineered where standard gel loading buffers fall short
Conventional 0.22 µm-filtered gel loading buffers can carry subvisible particulates, undetected nuclease activity, and inconsistent dye/glycerol formulation — all of which can compromise band resolution, sample loss, or downstream nucleic acid integrity. FluxMPS™ Gel Loading Solution [6X] is built to remove those failure modes at the source.
Microchannel & Gel-Safe Purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration reduces particulates that can interfere with clean electrophoretic band migration.
Precise, Reproducible Loading Density
A 30% glycerol formulation delivers consistent sample density so DNA/RNA samples sink reliably to the bottom of the well, run after run.
Ultrapure Type 1 Water Base
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) consistent with USP <85> water-quality expectations for sensitive nucleic acid work.
Built-In Visual Tracking
Bromophenol blue and xylene cyanol FF provide dual-dye visual monitoring of electrophoresis progress in real time.
Defined, Traceable Composition
Glycerol and tracking-dye content are formulated to a defined composition with lot-level quality control testing.
Customization on Demand
Alternate concentrations, additional tracking dyes, different pH, and other modifications are available on request.
Quadruple-stage filtration system
DCP-GLS6X undergoes sequential 0.1 µm and 0.04 µm membrane filtration — each applied twice — for ultraclean, sterile performance in DNA and RNA gel loading workflows.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the life of downstream filtration stages.
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2
0.04 µm Pre-filtration II
Retains finer particulates and bioburden ahead of final sterile filtration.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant particulate and bioburden control.
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4
0.04 µm Sterile-filtration II — Final Polish
A second 0.04 µm pass provides the final polish in an ISO 13485-certified, CE-approved aseptic environment.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single-pass 0.22 µm filtration typical of conventional gel loading buffers.
![Quadruple-stage 0.1 micron and 0.04 micron membrane filtration diagram for FluxMPS Gel Loading Solution [6X] DCP-GLS6X, an ultrapure sterile buffer for DNA and RNA electrophoresis and organ-on-a-chip nucleic acid workflows, manufactured by Diagnocine](https://diagnocine.com/Content/Upload/Buffer/Quadruple-stage.png "FluxMPS™ Quadruple-Stage Filtration System ? Gel Loading Solution [6X] for DNA/RNA Electrophoresis and Organ-on-a-Chip Applications | Diagnocine")
© Diagnocine® — DCP-GLS6X
Where FluxMPS™ Gel Loading Solution [6X] performs
DCP-GLS6X supports sample preparation and visual tracking for DNA and RNA electrophoresis across a range of molecular biology, genomics, and microfluidic nucleic acid QC workflows.
Automated Bioreactors & Robotics
For high-throughput and automated liquid-handling workflows dispensing gel loading buffer at scale, an optional 0.01 µm (10 nm) ultra-filtered variant is available to further reduce particulate load on sensitive robotic dispensing systems.
- Total Particulate Exclusion — supports cleaner fluidic pathways in automated dispensing systems
- Valve & Sensor Protection — reduces particulate accumulation risk in automated liquid handlers
- Extended Perfusion Stability — supports consistent performance in continuous or automated workflows
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered variant is available by request — contact support@diagnocine.com.
Nucleic Acid QC for MPS & Chip Workflows
Supports visual, gel-based QC of DNA/RNA isolated from organ-on-a-chip and microphysiological system cultures.
DNA & RNA Sample Preparation for Gel Loading
Prepares DNA or RNA samples for loading into agarose or polyacrylamide gels; recommended usage is typically 1 part buffer to 5-9 parts sample.
iPSC-Derived Model Handling
Supports gel-based confirmation of nucleic acids extracted from iPSC-derived model systems.
Endothelial & Primary Cell Perfusion
Supports nucleic acid gel QC following extraction from endothelial and primary cell perfusion cultures.
Nucleic Acid Confirmation Alongside Blotting Workflows
Compatible with sample workflows where gel-separated nucleic acids are confirmed prior to blotting or immunodetection steps.
Microscopy & Optical Sensing Support
Confirms nucleic acid extraction quality that supports downstream live-cell imaging and optical sensing assays.
Specifications at a glance
Key physical, purity, storage, and regulatory parameters for DCP-GLS6X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Glycerol, bromophenol blue, and xylene cyanol FF in aqueous solution |
| Appearance | Blue-purple to violet color |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane (x2) and 0.04 µm membrane (x2) in a sterile environment |
| DNase Activity | None detected after 18-hour incubation with plasmid DNA at room temperature |
| RNase Activity | None detected after 18-hour incubation with ribosomal RNA at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Fill Environment | ISO Class 5 (Class 100) aseptic fill |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing Site | Diagnocine R&D and Quality Testing Center; Totowa, New Jersey, USA |
| Manufacturing QMS | ISO 13485-certified, CE-approved facility |
| Regulatory Alignment | ISO 13485:2016, CE-approved |
| Production Method | Filtered 0.1 µm membrane (x2) and 0.04 µm membrane (x2) |
| Intended Use | Research Use Only (RUO) — DNA/RNA gel electrophoresis sample loading |
Full composition
Component concentrations for DCP-GLS6X, expressed exactly as released.
| Component | CAS Number | Concentration |
|---|---|---|
| Glycerol | 56-81-5 | 30% |
| Bromophenol Blue | 115-39-9 | 0.25% |
| Xylene Cyanol FF | 2650-17-1 | 0.25% |
Manufacturing & compliance
DCP-GLS6X is manufactured, filled, and quality-tested under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
ISO Class 5 Fill & Finish
Final sterile filtration and fill are performed in an ISO Class 5 (Class 100) aseptic environment.
Micro-Batch Precision
Customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.
Sterility & Filtration USP <71>
Filtered 0.1 µm membrane (x2) and 0.04 µm membrane (x2) in a sterile environment.
Particulate Control USP <788>
Quadruple-stage sub-0.1 µm filtration supports particulate control objectives during sterile fill.
Nuclease-Free Verification
No DNase or RNase activity detected after 18-hour incubation testing at room temperature.
Documentation / CoA
Certificate of Analysis available on request, covering appearance, sterility/filtration, and DNase/RNase testing.
How DCP-GLS6X compares
A side-by-side look at DCP-GLS6X against conventional gel loading buffers.
| Parameter | DCP-GLS6X (FluxMPS™) | Conventional Buffer | Standard Alternative |
|---|---|---|---|
| Filtration Architecture | Quadruple-stage (0.1 µm x2 + 0.04 µm x2) | Single-pass 0.22 µm | Single-pass 0.22 µm |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Nuclease-Free Verification | check_circle | cancel | cancel |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Manufacturing QMS | ISO 13485:2016 certified | Not typically certified | Varies by supplier |
| Microfluidic / Chip Compatibility | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-GLS6X.
Supporting literature
Curated literature relevant to gel loading buffer chemistry, nucleic acid electrophoresis, and sterile filtration practices.
- Lee, P. Y. et al. Agarose gel electrophoresis for the separation of DNA fragments. J. Vis. Exp. doi:10.3791/3923
- Sambrook, J. & Russell, D. W. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis. Cold Spring Harb. Protoc. doi:10.1101/pdb.top133
- Aranda, P. S. et al. Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis doi:10.1002/elps.201200335
- Voytas, D. Agarose gel electrophoresis. Curr. Protoc. Mol. Biol. doi:10.1002/0471142727.mb0205as51
- Barril, P. A. & Nates, S. V. Introduction to agarose and polyacrylamide gel electrophoresis matrices with respect to their detection sensitivities. Gel Electrophoresis - Principles and Basics doi:10.5772/38359
- Barbas, C. F. et al. Quantitation of DNA and RNA. Cold Spring Harb. Protoc. doi:10.1101/pdb.ip84
- Rio, D. C. et al. Nondenaturing polyacrylamide gel electrophoresis of RNA. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot5445
- Bustin, S. A. et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. doi:10.1373/clinchem.2008.112797
- Low, L. A. et al. Organs-on-chips: into the next decade. Nat. Rev. Drug Discov. doi:10.1038/s41573-020-0079-3
- Rios, A. C. & Clevers, H. Imaging organoids: a bright future ahead. Nat. Methods doi:10.1038/s41592-018-0130-7