Gel Loading Non-denaturing Buffer [6X]
Cat No.: DCP-GLNDB6X
Size: 5 ml
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
6X Gel Loading Buffer is a specialized reagent used to prepare nucleic acid samples for loading onto agarose or polyacrylamide gels during electrophoresis. This buffer is widely used in laboratories for preparing DNA or RNA samples for gel electrophoresis, including applications such as restriction fragment analysis, PCR product analysis, and DNA ladder preparation. Its formulation ensures reliable performance and ease of use, making it a staple in nucleic acid research and analysis. Sucrose acts as a density agent, ensuring that samples sink into the wells of the gel. The presence of sucrose also helps maintain the integrity of the samples during the electrophoresis process. The tracking dyes migrate alongside the nucleic acids, providing a visual reference to monitor the progress of electrophoresis and indicating when the bands are optimally separated. EDTA protects nucleic acids from degradation by inhibiting enzymes that could compromise sample integrity.
Applications
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Sample preparation: The buffer is mixed with DNA or RNA samples before loading them onto agarose or polyacrylamide gels.
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Versatile: Suitable for DNA, RNA and native protein samples.
Advantages:
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Density increase: The high concentration of sucrose (usually around 40%) increases the density of samples, allowing them to sink to the bottom of the gel wells.
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Tracking dye: Contains dyes like bromophenol blue that migrate through the gel, allowing visual tracking of sample migration.
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Higher concentration: The 6X concentration allows for larger sample volumes to be loaded without diluting the DNA too much.
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Sharp bands: Sucrose can help produce well-defined, sharp DNA bands on the gel.
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Compatibility: Works well with both agarose and polyacrylamide gels.
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Non-denaturing: Unlike formamide-based buffers, sucrose loading buffers are non-denaturing and maintain the native structure of DNA
Composition:
| Ingredients |
Concentration |
| Sucrose |
40% |
| EDTA |
0.1 M |
| SDS |
0.5% |
| Bromophenol Blue |
0.05% |
Quality Control:
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Appearance: Blue-purple
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pH: 8.0
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
