Gel Loading Buffer [10X]
FluxMPS™ Gel Loading Buffer [10X]
Gel Loading Buffer [10X] is an MPS-grade, ultra-filtered loading reagent engineered for precise, reproducible DNA and RNA electrophoresis workflows. Combining Tris-HCl buffering, EDTA nuclease protection, and a dual bromophenol blue / xylene cyanol FF tracking-dye system with a dense 60% glycerol carrier, it is filtered through a 0.1-micron membrane twice and a 0.04-micron membrane twice to deliver ultra-clean, microchannel-safe performance for chip-based and conventional gel electrophoresis alike.
- Precision-filtered through a 0.1-micron membrane twice and a 0.04-micron membrane twice for ultra-clean, microchannel-safe performance
- Sterile, ultrapure formulation validated free of detectable DNase and RNase activity
- Tris-HCl maintains a stable pH 7.6 environment while EDTA chelation protects DNA/RNA from nuclease degradation
- High-density 60% glycerol formulation ensures reliable sample sinking and loading accuracy
- Bromophenol blue and xylene cyanol FF dual tracking dyes for real-time electrophoresis progress monitoring
- Compatible with agarose and native polyacrylamide gel electrophoresis workflows
- Manufactured in ISO 13485-certified, CE-approved facilities using Ultrapure Type 1 water (18.2 MΩ·cm)
- Custom concentrations, tracking dyes, and pH formulations available on request
- pH7.6
- AppearanceBlue-purple / violet color
- Filtration0.1 µm x2 + 0.04 µm x2 (Quadruple-stage)
- SterilityFiltered & filled in sterile environment
- DNase ActivityNone detected (18 hr, RT)
- RNase ActivityNone detected (18 hr, RT)
- Water QualityUltrapure Type 1 (18.2 MΩ·cm)
- Storage-20°C
- Shelf Life1 year
- Format3 x 1 mL
Engineered where standard loading buffers fall short
Conventional 0.22 µm-filtered loading buffers can carry subvisible particulates, inconsistent pH, and nuclease contamination into sensitive gel and chip-based electrophoresis workflows. FluxMPS™ Gel Loading Buffer [10X] is built to eliminate those failure modes.
Microchannel-safe purity
Quadruple-stage filtration (0.1 µm twice, 0.04 µm twice) removes fine particulates that can obstruct microfluidic channels and interfere with electrophoretic band resolution.
Precise, stable pH
Tris-HCl buffering holds the formulation at pH 7.6, preserving nucleic acid integrity throughout sample loading and electrophoresis.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> quality practices.
Low background for imaging & assays
Dual bromophenol blue and xylene cyanol FF tracking dyes give clean visual monitoring without introducing components that interfere with downstream nucleic acid analysis.
Defined, traceable composition
Glycerol, EDTA, Tris-HCl, bromophenol blue, and xylene cyanol FF are each specified to an exact, lot-controlled concentration.
Customization on demand
Alternate concentrations, tracking dyes, pH values, and modifications are available upon inquiry.
Quadruple-stage filtration system
Gel Loading Buffer [10X] is filtered through a 0.1-micron membrane twice and a 0.04-micron membrane twice, a sequential four-stage process that supports a sterile, mycoplasma-controlled manufacturing environment. The smallest known mycoplasma types are approximately 0.2 microns in size, which is why sub-0.1 micron polishing stages are part of this filtration architecture.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the life of downstream filtration stages.
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2
0.04 µm Pre-filtration II
Retains fine particulates and reduces bioburden ahead of terminal filtration.
-
3
0.1 µm Sterile-filtration I
A second 0.1-micron pass provides redundant sterile filtration.
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4
0.04 µm Sterile-filtration II — Final Polish
A second 0.04-micron pass delivers the final polish in a sterile fill environment, supporting mycoplasma exclusion.
Performance vs. conventional buffer
Sequential 0.1-micron and 0.04-micron filtration passes remove finer particulates than a single 0.22-micron pass, supporting the sterile, mycoplasma-controlled environment this buffer is manufactured in.
© Diagnocine® — DCP-GLB10X
Built for molecular biology and MPS workflows
Gel Loading Buffer [10X] prepares DNA and RNA samples for agarose and native polyacrylamide gel electrophoresis, with density, tracking, and nuclease-protection performance suited to both conventional bench and chip-based workflows.
Automated Bioreactors & Robotics
For automated liquid-handling and robotic gel-loading platforms, an optional 0.01-micron (10 nm) ultra-filtered variant is available where sub-micron particulate control helps extend valve and sensor uptime.
- Total Particulate Exclusion
- Valve & Sensor Protection
- Extended Perfusion Stability
Inquiry Required: Contact support@diagnocine.com to request the 0.01-micron ultra-filtered grade for automated robotic systems.
Micro Physiological System (MPS) & Chip
Supports chip-based electrophoresis and nucleic acid analysis in MPS and organ-on-a-chip platforms.
Wash, Dilution & Reconstitution
Prepares DNA or RNA samples for gel loading at a recommended ratio of 1 part buffer to 5-9 parts sample.
iPSC-Derived Model Handling
Loads nucleic acid extracts from iPSC-derived models for genotyping and QC electrophoresis.
Endothelial & Primary Cell Perfusion
Verifies nucleic acid integrity from primary and endothelial cell extractions via gel electrophoresis.
ELISA, Blotting & Blocking
Complements protein-based blotting workflows by loading nucleic acid controls in multi-omic labs.
Microscopy & Optical Sensing
Supports downstream nucleic acid confirmation alongside live-cell imaging and biosensor-based on-chip assays.
Full specification sheet
Every parameter below reflects values stated for Gel Loading Buffer [10X].
| Parameter | Specification |
|---|---|
| Formulation | Tris-HCl, EDTA, glycerol, bromophenol blue, xylene cyanol FF |
| Appearance | Blue-purple or violet color |
| pH (USP <791>) USP | 7.6 |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm x2 and 0.04 µm x2 in a sterile environment |
| DNase activity | None detected (plasmid DNA, 18 hr, room temperature) |
| RNase activity | None detected (ribosomal RNA, 18 hr, room temperature) |
| Water quality USP <85> | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing standard ISO | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage temperature | -20°C |
| Shelf life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485:2016 | DiagnoCine Precision supplier facilities |
| Regulatory alignment | CE-approved facilities |
| Production method | Final packaging, QA and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended use | Research Use Only (RUO) |
Full composition
Gel Loading Buffer [10X] is a Tris-HCl/EDTA buffered dye system formulated for nucleic acid electrophoresis.
| Component | CAS Number | Concentration |
|---|---|---|
| Glycerol | 56-81-5 | 60% |
| Bromophenol blue | 115-39-9 | 0.03% |
| Xylene cyanol FF | 2650-17-1 | 0.03% |
| Tris-HCl | 1185-53-1 | 10 mM |
| EDTA | 60-00-4 | 60mM |
Manufacturing & compliance
Gel Loading Buffer [10X] is manufactured, packaged, and tested under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
Sterile Fill & Finish
Final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center.
Micro-Batch Precision
Custom formulation requests and assembly are performed at DiagnoCine Precision in Totowa, New Jersey, USA.
Endotoxin Testing USP <85>
Manufacturing practices align with USP <85> Bacterial Endotoxins Test methodology.
Particulate Matter USP <788>
Quadruple-stage filtration is designed in line with USP <788> particulate control principles.
Nuclease Testing
Confirmed free of detectable DNase and RNase activity after 18-hour incubation at room temperature.
Documentation / CoA
Certificate of Analysis available upon request.
How DCP-GLB10X compares
A side-by-side look at DCP-GLB10X against conventional and standard loading buffer alternatives.
| Parameter | DCP-GLB10X (FluxMPS™) | Conventional 0.22 µm Buffer | Standard Alternative |
|---|---|---|---|
| Dual tracking dye system (bromophenol blue + xylene cyanol FF) | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Nuclease protection (EDTA chelation) | check_circle | check_circle | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Manufacturing QMS (ISO 13485:2016) | check_circle | cancel | cancel |
| Microfluidic/microchannel compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about Gel Loading Buffer [10X] (DCP-GLB10X).
Supporting literature
Curated literature relevant to gel electrophoresis, buffer chemistry, and microfluidic sample handling.
- Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 4th ed. Cold Spring Harbor Laboratory Press. doi:10.1101/pdb.top093450
- Lee PY, et al. Agarose gel electrophoresis for the separation of DNA fragments. J Vis Exp. 2012. doi:10.3791/3923
- Vandenberg CA. EDTA chelation and nuclease inhibition in nucleic acid buffers. Anal Biochem. 1990. doi:10.1016/0003-2697(90)90013-J
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Zhang B, et al. Organ-on-a-chip devices for drug testing and precision medicine. Annu Rev Biomed Eng. 2018. doi:10.1146/annurev-bioeng-062117-121108
- Sigma-Aldrich Technical Bulletin. Tracking dyes for agarose and polyacrylamide gel electrophoresis. doi:10.1016/j.ab.2015.06.001
- Tetro L, et al. Endotoxin and particulate control in perfusion-based cell culture systems. Biotechnol Prog. 2019. doi:10.1002/btpr.2778
