Gel Loading Buffer [10X]
Cat No.: DCP-GLB10X
Size: 3 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Gel Loading Buffer (10X) is an essential component for DNA and RNA electrophoresis experiments. It facilitates the loading process by increasing the density of the sample and providing visual tracking. Tris-HCl provides a stable pH environment for the DNA/RNA samples and helps maintain the integrity of nucleic acids during electrophoresis. EDTA acts as a chelating agent and inhibits metal-dependent nucleases, protecting DNA/RNA from degradation. Bromophenol blue serves as a tracking dye and allows visual monitoring of the electrophoresis progress. Glycerol increases the density of the sample and ensures that the DNA/RNA sample sinks to the bottom of the well when loaded. Gel Loading Buffer (10X) formulation is suitable for both agarose and native polyacrylamide gel electrophoresis, providing a versatile solution for various nucleic acid separation experiments.
* Please note that this buffer contains bromophenol blue.
Advantages:
- Sample density: The high glycerol content (60%) increases the density of the sample, ensuring it sinks to the bottom of the well when loaded onto the gel. This prevents sample loss and improves loading accuracy.
- Nucleic acid protection: EDTA acts as a chelating agent, inhibiting metal-dependent nucleases and protecting DNA/RNA from degradation during electrophoresis.
- Visual tracking: The bromophenol blue serves as a tracking dye, allowing researchers to monitor the progress of electrophoresis visually. In a 1% agarose gel, bromophenol blue migrates with DNA fragments of approximately 300-500 base pairs.
Application:
- Sample preparation: It's used to prepare DNA or RNA samples for loading into agarose or polyacrylamide gels. The recommended usage is typically 1 part buffer to 5-9 parts sample, depending on the specific application.
- Density increase: The high glycerol content (50-60%) increases the density of the sample, ensuring it sinks to the bottom of the well when loaded onto the gel. This prevents sample loss and improves loading accuracy.
- Versatility: This buffer is compatible with both agarose and native polyacrylamide gels, making it suitable for various nucleic acid separation experiments.
- Compatibility with downstream applications: It doesn't introduce components that might interfere with subsequent analyses of the separated nucleic acids.
Composition:
| Ingredients |
Concentration |
| Glycerol |
60% |
| Bromophenol blue |
0.03% |
| Xylene cyanol FF |
0.03% |
| Tris-HCl |
10 mM |
| EDTA |
60mM |
Quality Control:
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Appearance: blue-purple or violet color.
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pH: 7.6
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
