FluxMPS Waymouth Medium w/o Sodium Bicarbonate: 1X Liquid

Q: What makes the filtration process different from standard 0.22 micron filtered media?

FluxMPS runs two 0.1 micron prefilter plus 0.04 micron final-filter pairs in series - Prefiltration I, Final filtration I, Prefiltration II, Final filtration II - reaching a 0.04 micron final cut-off, five times finer than the 0.22 micron membranes used in conventional sterile filtration.

Q: Does DCP-WMBM-B1X require a CO2 incubator?

No. This formulation is CO2-independent and phosphate-buffered, suitable for ambient-air incubation. Supplement with HEPES (15-25 mM) for additional pH buffering if the culture format requires it.

Q: Is a Certificate of Analysis (CoA) available, and what does it contain?

Yes. Full CoA per batch covers: appearance, pH, osmolality, sterility, endotoxin, particulate count (USP less than 788 Method 1), and raw-material traceability. Request at support@diagnocine.com.

verified ISO 13485 Certified Manufacturing

FluxMPS™ Waymouth Medium w/o Sodium Bicarbonate

L-Glutamine Phenol Red Calcium Magnesium Glucose Sodium Bicarbonate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Waymouth Medium MB 752/1 formulated without sodium bicarbonate, for CO₂-independent culture of hybridomas, fibroblasts, and other mammalian cell lines on microfluidic, organ-on-a-chip (OoC), and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Glucose (5 g/L), [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate.

Waymouth MB 752/1 formulation with 5000 mg/L (5.0 g/L) D-glucose as the primary carbon source
350 mg/L L-glutamine plus 15 mg/L reduced glutathione for nitrogen and redox support
Formulated without sodium bicarbonate; phosphate-buffered for CO₂-independent culture (HEPES supplementation optional)
Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), verified per manufacturing batch
Contains phenol red sodium salt (11 mg/L) as a pH indicator; red-colored, clear solution
Manufactured under an ISO 13485:2016 quality management system with full lot traceability
40 characterized components across inorganic salts, amino acids, vitamins, and specialty additives, released with a per-lot Certificate of Analysis

CAT. NO.

DCP-WMBM-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)

Waymouth Medium MB 752/1 — 1X Liquid

Media familyWaymouth Medium MB 752/1
Glucose5000 mg/L (5.0 g/L)
Formulation[+] Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Bicarbonate
AppearanceRed-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)240 – 345 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Storage2–8°C, protect from light
Shelf Life12 months from date of manufacture, unopened

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Waymouth Medium passes subvisible particulates and organisms that clog microfluidic channels and interfere with sensitive assays. FluxMPS™ is built to a finer standard.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance, together with a < 0.05 EU/mL endotoxin release specification (USP <85>), supports use with TLR4-sensitive primary cell types and narrow-geometry microfluidic channels.

target

Defined nutrient control

5000 mg/L D-glucose, 350 mg/L L-glutamine, and 15 mg/L reduced glutathione provide a phosphate-buffered nutrient base for hybridoma, fibroblast, and other demanding cell types requiring the Waymouth MB 752/1 formulation.

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Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with trace-metal and organic-carbon (TOC) control minimizes trace contaminant carryover into every formulation.

visibility

Low background for imaging

The 0.04 µm final filtration establishes an ultra-low particulate baseline suited to confocal microscopy and biosensor-based assays; phenol-red-free formulations are available on request for fluorescence-sensitive applications requiring minimal background.

science

Rich, stable nutrient profile

40 characterized components across inorganic salts, amino acids, vitamins, and specialty additives — including 15 mg/L reduced glutathione — released via micro-batch, per-lot quality control.

tune

Customization on demand

pH, nutrient concentrations, HEPES buffering, and other component modifications available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two prefilter + final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Prefiltration I

Removes large aggregates and particulate; protects the first 0.04 µm cartridge.
2

0.04 µm Final filtration I

First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
3

0.1 µm Prefiltration II

A second, dedicated prefilter protecting the second 0.04 µm cartridge.
4

0.04 µm Final filtration II — Polish

Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Purity architecture, stated plainly

FluxMPS™ DCP-WMBM-B1X runs two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs in series, ending at a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration.

4

Filtration passes (two prefilter + final-filter pairs)

0.04

µm final pore size

Sterility & size-exclusion: No growth after 14-day incubation (USP <71>). The 4-stage train (0.1 µm ×2 + 0.04 µm ×2) is sized to retain organisms in the 0.2–0.3 µm range, including mycoplasma; mycoplasma testing is not performed on every lot.

Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.

FluxMPS DCP-WMBM-B1X Waymouth Medium Quadruple-stage filtration system, four stages (0.1 micron x2 + 0.04 micron x2), Microfluidics Suitable for organ-on-a-chip and microphysiological system applications, Diagnocine — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) used in DCP-WMBM-B1X production.
© Diagnocine® — DCP-WMBM-B1X

Applications

Specialized cell culture applications

FluxMPS™ DCP-WMBM-B1X — Waymouth Medium MB 752/1 without sodium bicarbonate — delivers 0.04 µm filtered, Microfluidics Suitable purity for mammalian cell culture and related microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor and robotic perfusion systems.

Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Bioproduction

CHO & Mammalian Cell Culture

Supports CHO, hybridoma, myeloma, and primary fibroblast culture in serum-free or low-serum conditions.

CHOHybridomaMyeloma

Microfluidics

Organ-on-a-Chip & MPS

Ultra-filtered to a 0.04 µm final pore size, reducing particulate load and channel-obstruction risk in complex multi-organ chip architectures.

OoCToCMPS

Cancer Biology

Cancer Cell Lines

Supports fibroblast co-culture tumor models and primary cancer cell culture in the defined nutrient environment of Waymouth Medium.

Tumor modelsFibroblastsCo-culture

Stem Cell Biology

Primary & Stem Cell Support

Rich nutrient profile with trace elements supports primary cell viability and stem cell maintenance in low-serum conditions.

Primary cellsMSCsiPSC co-culture

Metabolomics

Metabolic Flux Analysis

Defined, phosphate-buffered nutrient base supports ¹³C isotope tracing and other metabolic flux workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingMetabolomics

Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal microscopy, TEER measurement, and biosensor-based assays; phenol-red-free variants available on request.

ConfocalTEERBiosensors

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium \| [-] Sodium Bicarbonate
Appearance	Red-colored, clear solution
Glucose	5000 mg/L (5.0 g/L)
L-Glutamine	350 mg/L
Phenol Red	11 mg/L (phenol red sodium salt)
pH USP <791>	7.4
Osmolality USP <785>	240 – 345 mOsm/kg H₂O
Total ingredients	40 (across 4 categories)

Sterility, Purity & Safety Parameters

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1	NMT 25/mL
Particulate ≥25 µm USP <788> Method 1	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from date of manufacture, unopened
Shipping condition	Cold pack
CO₂ requirement	CO₂-independent; phosphate-buffered; supplement with HEPES (15–25 mM) for additional pH buffering if required

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
UNSPSC	41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment	21 CFR Part 820 (QMSR) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

Waymouth Medium MB 752/1 (w/o sodium bicarbonate): 40 ingredients verified per lot with CAS numbers for full raw-material traceability.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	120.000
Disodium hydrogen phosphate anhydrous	7558-79-4	300.000
Magnesium chloride anhydrous	7786-30-3	112.560
Magnesium sulfate anhydrous	7487-88-9	97.720
Potassium chloride	7447-40-7	150.000
Potassium dihydrogen phosphate	7778-77-0	80.000
Sodium chloride	7647-14-5	6000.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	50.000
L-Arginine hydrochloride	1119-34-2	75.000
L-Aspartic acid	56-84-8	60.000
L-Cysteine hydrochloride monohydrate	7048-04-6	90.000
L-Cystine dihydrochloride	30925-07-6	19.550
L-Glutamic acid	56-86-0	150.000
L-Glutamine	56-85-9	350.000
L-Histidine hydrochloride monohydrate	5934-29-2	150.000
L-Isoleucine	73-32-5	25.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	240.000
L-Methionine	63-68-3	50.000
L-Phenylalanine	63-91-2	50.000
L-Proline	147-85-3	50.000
L-Threonine	72-19-5	75.000
L-Tryptophan	73-22-3	40.000
L-Tyrosine disodium salt dihydrate	69847-45-6	57.660
L-Valine	72-18-4	65.000

Component	CAS Number	mg/L
VITAMINS
Ascorbic acid	50-81-7	17.500
Biotin	58-85-5	0.020
Choline chloride	67-48-1	250.000
D-Ca-Pantothenate	137-08-6	1.000
Folic acid	59-30-3	0.400
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	1.000
Thiamine hydrochloride	67-03-8	10.000
Vitamin B12	68-19-9	0.200
i-Inositol	87-89-8	1.000
Hypoxanthine sodium salt		29.000
OTHERS
D-Glucose	50-99-7	5000.000
Glutathione reduced	70-18-8	15.000
Phenol red sodium salt	34487-61-1	11.000

Custom formulation: Contact support@diagnocine.com for DCP-WMBM-B1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm with trace-metal and organic-carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis issued for every batch.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm); light-obscuration method.

Osmolality — USP <785>

Target: 240 – 345 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:

Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
pH, osmolality, conductivity, appearance and clarity
Sterility

A Certificate of Analysis is available on request at support@diagnocine.com.

Product Comparison

How DCP-WMBM-B1X compares

FluxMPS™ DCP-WMBM-B1X vs. conventional 0.22 µm–filtered Waymouth formulations and standard DMEM/RPMI media.

Parameter	DCP-WMBM-B1X (FluxMPS™)	Conventional Waymouth Medium (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
Grade	Microfluidics Suitable	Not specified	Not specified
Waymouth MB 752/1 without sodium bicarbonate — CO₂-independent hybridoma and fibroblast culture	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma-retentive filtration	check_circle Yes (0.1 µm + 0.04 µm stages)	cancel No	cancel No
Endotoxin (release specification)	FluxMPS™ — < 0.05 EU/mL	Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatibility	check_circle Microfluidics Suitable	cancel Risk of clogging	cancel Risk of clogging
Custom formulation available	check_circle Yes	cancel No	cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-WMBM-B1X — Waymouth Medium MB 752/1 without sodium bicarbonate.

Is DCP-WMBM-B1X suitable for organ-on-a-chip (OoC) applications?expand_more

Yes. DCP-WMBM-B1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, making it Microfluidics Suitable for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and other microphysiological system (MPS) platforms culturing hybridomas, fibroblasts, and other mammalian cell lines.

What makes the filtration process different from standard 0.22 µm filtered media?expand_more

FluxMPS™ runs two 0.1 µm prefilter + 0.04 µm final-filter pairs in series — Prefiltration I, Final filtration I, Prefiltration II, Final filtration II — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.

Why is sodium bicarbonate excluded, and how do I buffer pH?expand_more

Sodium bicarbonate is excluded so this formulation can be used in CO₂-independent (ambient air) conditions, relying on its phosphate buffering system. If additional buffering capacity is needed — for example in open-top chips or long perfusion runs — supplement with HEPES (15–25 mM), which is not included in this formulation. Contact support@diagnocine.com for a HEPES-supplemented variant.

Does DCP-WMBM-B1X require a CO₂ incubator?expand_more

No. This formulation is CO₂-independent and phosphate-buffered, suitable for ambient-air incubation. Supplement with HEPES (15–25 mM) for additional pH buffering if the culture format requires it.

Can DCP-WMBM-B1X be supplemented with serum, growth factors, or other components?expand_more

Yes. Add FBS, serum-free supplements, growth factors, or antibiotics as required. When adding serum or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter rather than a 0.04 µm membrane, which will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.

What endotoxin level should I expect, and how is it verified?expand_more

Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) and must meet a release specification of < 0.05 EU/mL before release. A Certificate of Analysis is available on request.

Is a Certificate of Analysis (CoA) available, and what does it contain?expand_more

Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting Waymouth Medium MB 752/1 in specialized cell culture and OoC applications.

Waymouth C. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). J Natl Cancer Inst. 1959;22:1003–1017. doi:10.1093/jnci/22.5.1003
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Waymouth Medium w/o Sodium Bicarbonate: 1X Liquid