FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-RPMI-QPB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation supplying 2.0 g/L glucose as the sole added carbon source, with L-glutamine, sodium pyruvate, and sodium bicarbonate all left out so investigators can define their own metabolic and buffering conditions. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Carbon source: 2000 mg/L (2.0 g/L) D-glucose — the sole added energy substrate in this triple-omission formulation
- Formulated without L-glutamine, sodium pyruvate, and sodium bicarbonate for maximum user-defined metabolic and buffer control
- Contains 1.0 mg/L reduced glutathione — antioxidant support for redox-sensitive lymphocyte and hematopoietic cell types
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, with an endotoxin release specification of < 0.05 EU/mL (LAL, USP <85>)
- CO₂-independent formulation; supplement with 15–25 mM HEPES for pH buffering under atmospheric conditions
- Manufactured under an ISO 13485:2016 quality management system, with Ultrapure Type 1 water (18.2 MΩ·cm) and aseptic ISO Class 5 fill & finish
- 38 formulation components verified per lot across inorganic salts, amino acids, vitamins, and other constituents, with full CAS traceability
- Media familyRPMI 1640
- Formulation[+] Phenol Red, Calcium, Magnesium, 2.0 g/L Glucose | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate
- L-GlutamineNone / Not added
- Sodium PyruvateNone / Not added
- pH (USP <791>)7.4
- Osmolality (USP <785>)230–270 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes subvisible particulates and mycoplasma-sized organisms that can activate TLR4 signaling and confound immune cell assays. FluxMPS™ is built to reduce those failure modes.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 particulate compliance keep suspension immune cell channels free of clogging debris.
Total metabolic control
User-defined carbon source: only 2.0 g/L glucose is provided, with glutamine, pyruvate, and bicarbonate left to the investigator for precise control of lymphocyte metabolic-flux experiments.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) minimizes trace-metal and organic-carbon content entering the formulation.
Low background for imaging
0.04 µm filtration minimizes the subvisible particulate baseline that can scatter light and interfere with confocal microscopy and biosensor-based readouts, independent of formulation color.
Rich, stable nutrient profile
38 verified components across amino acids, vitamins, inorganic salts, and other constituents — including reduced glutathione for redox-sensitive hematopoietic cell types — released on a per-lot micro-batch basis.
Customization on demand
pH, HEPES, glucose concentration, and re-addition of glutamine, pyruvate, or bicarbonate available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration passes — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.
-
1
0.1 µm Prefiltration I
Large particulate, cell debris, and protein aggregate removal; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 (Class 100) environment.
Performance vs. conventional media
© Diagnocine® — DCP-RPMI-QPB1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMI-QPB1X — RPMI 1640 — delivers 0.04 µm filtered purity for hematopoietic and lymphocyte cell models and related microfluidic applications.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated perfusion and robotic handling systems.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard base medium for primary T cells, B cells, NK cells, and monocytes. 0.04 µm filtration reduces particulate-driven TLR4 activation artifacts in immune assays.
Leukemia & Lymphoma Lines
RPMI supports NCI-60 hematopoietic cancer lines, Jurkat, Raji, K562, and HL-60 — models where a DMEM background would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models, where particle-free media reduces TLR4 activation risk.
CAR-T & TIL Expansion
A low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols where minimizing LPS-driven T cell activation artifacts is a priority.
Immune Cell Metabolic Flux
Defined RPMI base for lymphocyte glycolysis and OXPHOS flux studies and ¹³C isotope tracing of T cell activation metabolism. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium — this formulation contains phenol red.
Flow Cytometry & Confocal
Ultra-low particulate 0.04 µm filtration reduces background scatter for flow cytometry, ELISA, and immune cell confocal imaging. A phenol-red-free custom variant is available on request for fluorescence-sensitive assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Phenol Red, Calcium, Magnesium, 2.0 g/L Glucose | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate |
| Appearance | Orange-colored, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 230–270 mOsm/kg H₂O |
| Total ingredients | 38 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release spec) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent (bicarbonate-free); supplement with 15–25 mM HEPES for pH buffering |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640: 38 ingredients verified per lot with CAS numbers for full raw-material traceability, including reduced glutathione for redox-sensitive lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6000.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.00 |
| Pyridoxine hydrochloride | 58-56-0 | 1.00 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 35.000 |
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| Phenol red sodium salt | 34487-61-1 | 5.300 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine's R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity feedwater minimizes trace-metal and organic-carbon content in every batch.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm) by light obscuration.
Osmolality — USP <785>
Target: 230–270 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-RPMI-QPB1X compares
FluxMPS™ DCP-RPMI-QPB1X specifications set against published supplier data where available.
| Parameter | DCP-RPMI-QPB1X (FluxMPS™) | Published Comparison |
|---|---|---|
| Grade | Microfluidics Suitable | Not specified |
| Formulation | Triple-omission RPMI 1640 (no L-glutamine, no sodium pyruvate, no sodium bicarbonate) | Not specified |
| Final filtration pore size | 0.04 µm | Not specified |
| Number of filtration stages | 4 (Quadruple-stage) | Not specified |
| Mycoplasma control | 0.1 µm mycoplasma-retentive filtration (not tested per lot) | Not specified |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| USP <788> particulate compliance | Tested, Method 1 (light obscuration) | Not specified |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Not specified |
| Manufacturing QMS | ISO 13485:2016 | Not specified |
| Microfluidic channel compatible | check_circle Yes | Not specified |
| Custom formulation | check_circle Available on request | Not specified |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMI-QPB1X — RPMI 1640.
Supporting literature
Key publications supporting RPMI 1640 in immune cell culture and OoC applications.
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- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Ma C, et al. Organ-on-a-chip: a new paradigm for drug development. Trends Pharmacol Sci. 2021;42:119–133. doi:10.1016/j.tips.2020.11.009
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
- Junkin M, et al. High-content quantification of single-cell immune dynamics. Cell Rep. 2016;15:411–422. doi:10.1016/j.celrep.2016.03.033