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FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMIH-BR1X
$44.00
DCP-RPMIH-BR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium with 25mM HEPES, w/o Sodium Bicarbonate & Phenol Red — 1X Liquid

Contains L-Glutamine Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate Without Phenol Red

FluxMPS™ DCP-RPMIH-BR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 + 25 mM HEPES formulation engineered for hematopoietic cells, lymphocytes and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The quadruple-stage train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides CO2-independent pH buffering; this formulation contains no sodium bicarbonate and no phenol red. Formulation: [+] L-Glutamine, [+] HEPES, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] Sodium Bicarbonate, [-] Phenol Red.

  • Glucose 2000 mg/L (2.0 g/L) with L-glutamine (300 mg/L) and sodium pyruvate (110 mg/L) as carbon/energy sources
  • 25 mM HEPES (pKa 7.3 at 37°C) provides robust, CO2-independent pH buffering for open-air handling and flow cytometry workflows
  • Glutathione (reduced, 1 mg/L) included for antioxidant/redox support in redox-sensitive immune cell types
  • Bicarbonate-free and phenol red–free formulation avoids autofluorescence background for PE-channel flow cytometry and confocal imaging
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, with 0.1 µm mycoplasma-retentive filtration at each pass
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ
  • pH, HEPES concentration, and nutrient composition available on request for custom immune-cell formulations — contact support@diagnocine.com
CAT. NO.
DCP-RPMIH-BR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 + 25mM HEPES — 1X Liquid
  • Glucose2000 mg/L (2.0 g/L)
  • L-Glutamine300 mg/L
  • Sodium Pyruvate110 mg/L
  • HEPES25 mM (pKa 7.3 at 37°C)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • FiltrationQuadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that can activate TLR4 signaling and confound immune-cell assays. FluxMPS™ is built to address these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration reaches USP <788> Method 1 (light obscuration) particulate compliance, and the 0.1 µm stages provide mycoplasma-retentive filtration. Particle-free media for suspension immune-cell OoC models and flow cytometry workflows.

science

Immune cell–optimized formulation

RPMI 1640 contains glutathione (reduced) and a balanced profile of vitamins and amino acids formulated for lymphocyte, T-cell and hematopoietic cell culture.

water_drop

Ultrapure-grade water

Type 1 water (18.2 MΩ·cm) with trace-metal and organic-carbon (TOC) control, reducing feed-water contamination risk in sensitive immune-cell cultures.

visibility

Low background for imaging

Phenol red–free formulation combined with an ultra-low particulate baseline supports confocal imaging, flow cytometry and biosensor-based readouts without particulate interference.

shield

Below the TLR4 activation threshold

Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>) helps avoid TLR4/NF-κB-driven cytokine artefacts that can confound T-cell and NK-cell activation assays.

tune

Customization on demand

pH, HEPES concentration, nutrient composition and additional components available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two 0.1 µm prefilter / 0.04 µm final-filter pairs run in series — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including mycoplasma-sized organisms (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill and finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration — with 0.1 µm mycoplasma-retentive filtration applied at every pass.

0.04
µm final pore size — sub-mycoplasma polishing
4
Serial filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & mycoplasma control: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 Medium 25mM HEPES DCP-RPMIH-BR1X quadruple-stage 0.1 micron x2 plus 0.04 micron x2 filtration system for organ-on-a-chip OoC and microfluidic immune cell applications - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIH-BR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMIH-BR1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered, bicarbonate-free, phenol red–free purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation is available on request for automated bioreactor and robotics platforms.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard base for primary T cells, B cells, NK cells and monocytes. 0.04 µm filtration reduces particulate-driven TLR4 activation that can confound immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60 and other hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip models where particle-free media reduces TLR4 activation risk.

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Low endotoxin release specification (<0.05 EU/mL) supports CAR-T manufacturing and TIL expansion protocols sensitive to LPS-driven activation artefacts.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base for Seahorse XF T-cell metabolic assays, glycolysis stress tests, and 13C isotope tracing of lymphocyte activation states.

Seahorse XF13C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate, phenol red–free formulation avoids autofluorescence for PE-channel flow cytometry and immune-cell confocal imaging.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine (300 mg/L), [+] HEPES (25 mM), [+] Calcium (100 mg/L as calcium nitrate), [+] Magnesium (48.84 mg/L as magnesium sulfate), [+] Glucose (2.0 g/L), [+] Sodium Pyruvate (110 mg/L) | [-] Sodium Bicarbonate, [-] Phenol Red
Appearance Pale yellow–colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H2O
Total ingredients 40 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 NMT 25/mL
Particulate ≥25 µm USP <788> M1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 + 25mM HEPES: 40 ingredients across 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others), released per lot with CAS numbers for raw-material traceability. RPMI 1640 contains glutathione (reduced) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.

Inorganic Salts
Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulphate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Amino Acids
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Vitamins and Others
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
i-Inositol 87-89-8 35.000
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.00
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIH-BR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, with trace-metal and organic-carbon (TOC) control.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis issued for every batch.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIH-BR1X compares

FluxMPS™ DCP-RPMIH-BR1X vs. conventional 0.22 µm–filtered RPMI 1640 formulations.

Parameter DCP-RPMIH-BR1X (FluxMPS™) Conventional RPMI 1640 + HEPES (0.22 µm filtered) Standard RPMI/DMEM (0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Formulation trait HEPES-buffered, bicarbonate-free, phenol red–free Usually bicarbonate-buffered Usually bicarbonate-buffered, phenol red–containing
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma barrier filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested (Method 1) check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes (Microfluidics Suitable) cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIH-BR1X — RPMI 1640 + 25mM HEPES.

Yes. DCP-RPMIH-BR1X is a Microfluidics Suitable formulation processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering an ultra-low particulate baseline for MPS, OoC and LoC platforms. HEPES buffering and phenol red–free, bicarbonate-free formulation suit immune cell OoC, tumor-immune interaction chips and lymphocyte perfusion models.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than a 0.22 µm membrane, with 0.1 µm mycoplasma-retentive filtration applied at every pass.
This formulation is HEPES-buffered and CO2-independent, and phenol red–free to avoid autofluorescence in fluorescence-based assays. L-Glutamine (300 mg/L) and sodium pyruvate (110 mg/L) are already included. It is well suited to atmospheric, fluorescence-based flow cytometry and imaging-monitored immune cultures; contact support@diagnocine.com if a bicarbonate-buffered or phenol red–containing version is required.
No. This formulation is CO2-independent — 25 mM HEPES alone maintains pH without gas supplementation, though it can still be used in a CO2 incubator if preferred.
Yes. FBS (typically 5–10%), cytokines, and other serum or protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm filter for serum, as it will retain IgM, lipoproteins and other serum components. Defined, protein-free additions may use a 0.1 µm filter. Contact support@diagnocine.com for custom co-formulation.
Every batch is released against a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch rather than per unit. Elevated endotoxin can activate TLR4/NF-κB signaling and confound T-cell and NK-cell activation assays, which is why a low, consistently enforced release specification matters for immune-cell work.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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