FluxMPS™ RPMI 1640 Medium with 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ RPMI 1640
FluxMPS™ DCP-RPMIH-QB1X is an MPS-grade, ultra-filtered RPMI 1640 + 25mM HEPES formulation engineered for Hematopoietic cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering. Formulation: [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Bicarbonate.
- Glucose source: 2000 mg/L (2.0 g/L)
- 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO₂
- Glutathione (reduced) included — antioxidant support for redox-sensitive cell types
- 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Media familyRPMI 1640 + 25mM HEPES
- Glucose2000 mg/L (2.0 g/L)
- HEPES25 mM, pKa 7.3 at 37°C
- Formulation[+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Bicarbonate
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)230.00–270.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months / 2–8°C
Engineered where standard media fails
Conventional 0.22 µm–filtered RPMI passes mycoplasma, subvisible particulates, and endotoxin fragments that activate TLR4 and confound immune cell assays. FluxMPS™ eliminates these failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance. Particle-free media for suspension immune cell OoC and flow cytometry.
Immune cell–optimized formulation
RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.
Ultrapure-grade water
18.2 MΩ·cm — eliminates ionic contaminants that alter lymphocyte signaling thresholds.
Below TLR4 endotoxin threshold
< 0.05 EU/mL — prevents LPS-driven T cell and NK cell activation artefacts in immune assays.
HEPES pH stability
HEPES (25 mM) prevents pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates; protects downstream 0.04 µm membranes.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-RPMIH-QB1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered RPMI, with confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-RPMIH-QB1X
Immune cell OoC and hematopoietic applications
FluxMPS™ DCP-RPMIH-QB1X — RPMI 1640 + 25mM HEPES — delivers 0.04 µm filtered purity for Hematopoietic cells and related OoC applications.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available on request.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
T Cell & Lymphocyte Culture
RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. FluxMPS 0.04 µm filtration prevents particulate TLR4 activation that confounds immune assays.
Leukemia & Lymphoma Lines
RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and all hematopoietic cancer lines where DMEM would alter proliferation and signaling.
Immune Cell OoC
0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip where particle-free media prevents TLR4 activation.
CAR-T & TIL Expansion
Ultra-low endotoxin (<0.05 EU/mL) prevents LPS-driven T cell activation artefacts in CAR-T manufacturing and TIL expansion protocols.
Immune Cell Metabolic Flux
Defined RPMI base for Seahorse XF T cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.
Flow Cytometry & Confocal
Ultra-low particulate; phenol red–free variants eliminate autofluorescence for PE-channel flow cytometry and immune cell confocal imaging.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] L-Glutamine, [-] Sodium Bicarbonate |
| Appearance | Orange-colored, clear solution |
| Glucose | 2000 mg/L (2.0 g/L) |
| HEPES | 25 mM (pKa 7.3 at 37°C) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 230.00–270.00 mOsm/kg H₂O |
| Total ingredients | 39 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | cold pack |
| CO₂ requirement | CO₂-independent — 25 mM HEPES alone maintains pH 7.2–7.4 at 37°C |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
RPMI 1640 + 25mM HEPES: 39 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium nitrate tetrahydrate | 13477-34-4 | 100.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 48.840 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6000.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 800.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 10.000 |
| L-Arginine hydrochloride | 1119-34-2 | 241.000 |
| L-Asparagine | 70-47-3 | 50.000 |
| L-Aspartic acid | 56-84-8 | 20.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 65.200 |
| L-Glutamic acid | 56-86-0 | 20.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Hydroxyproline | 51-35-4 | 20.000 |
| L-Isoleucine | 73-32-5 | 50.000 |
| L-Leucine | 61-90-5 | 50.000 |
| L-Lysine hydrochloride | 657-27-2 | 40.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 15.000 |
| L-Proline | 147-85-3 | 20.000 |
| L-Serine | 56-45-1 | 30.000 |
| L-Threonine | 72-19-5 | 20.000 |
| L-Tryptophan | 73-22-3 | 5.000 |
| L-Tyrosine Disodium Salt | 69847-45-6 | 28.830 |
| L-Valine | 72-18-4 | 20.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 3.000 |
| D-Biotin | 58-85-5 | 0.200 |
| D-Ca-Pantothenate | 137-08-6 | 0.250 |
| Folic acid | 59-30-3 | 1.000 |
| Niacinamide | 98-92-0 | 1.00 |
| Pyridoxine hydrochloride | 58-56-0 | 1.00 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 0.005 |
| i-Inositol | 87-89-8 | 35.000 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Glutathione reduced | 70-18-8 | 1.000 |
| HEPES | 7365-45-9 | 5958.00 |
| Phenol red sodium salt | 34487-61-1 | 5.300 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: 230.00–270.00 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available on request.
How DCP-RPMIH-QB1X compares
FluxMPS™ DCP-RPMIH-QB1X vs. conventional 0.22 µm–filtered RPMI formulations.
| Parameter | DCP-RPMIH-QB1X (FluxMPS™) | Conventional RPMI 1640 + 25m (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| RPMI 1640 + HEPES-only CO₂-free — fresh glutamine for atmospheric immune cell suspension | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| HEPES (25 mM) | check_circle Yes | cancel Usually no | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
Frequently asked questions
Common questions about FluxMPS™ DCP-RPMIH-QB1X — RPMI 1640 + 25mM HEPES.
Supporting literature
Key publications supporting RPMI 1640 + 25mM HEPES in immune cell culture and OoC applications.
- Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
