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FluxMPS™ RPMI 1640 Medium w/ L-Alanyl-Glutamine, 25mM HEPES: 1X Liquid

Product#: DCP-RPMIYAH1X
$50.00
DCP-RPMIYAH1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/ L-Alanyl-Glutamine, 25mM HEPES: 1X Liquid

Contains L-Alanyl-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Contains 2 g/L Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-RPMIYAH1X is a Microfluidics Suitable, ultra-filtered RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES formulation engineered for T cells and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] L-Alanyl-L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate. HEPES (25 mM, pKa 7.3 at 37°C) supplements the sodium bicarbonate buffering system for added pH stability.

  • RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES — L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) replaces free L-glutamine for stable, non-degrading nitrogen delivery
  • Glucose: 2000 mg/L (2.0 g/L); Sodium Pyruvate: 110 mg/L
  • 25 mM HEPES (pKa 7.3 at 37°C) supplements the sodium bicarbonate buffering system, reducing pH drift during open-bench handling and flow cytometry sampling
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, with mycoplasma-retentive filtration at every stage
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85>)
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa NJ
  • Custom formulation modifications available on request — contact support@diagnocine.com
CAT. NO.
DCP-RPMIYAH1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 Medium w/ L-Alanyl-Glutamine, 25mM HEPES: 1X Liquid
  • Glucose2000 mg/L (2.0 g/L)
  • Sodium Pyruvate110 mg/L
  • HEPES25 mM, pKa 7.3 at 37°C
  • Formulation[+] L-Alanyl-L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280 – 320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma, subvisible particulates, and endotoxin fragments that confound sensitive immune-cell culture applications. FluxMPS™ addresses these failure modes with four-stage filtration to a 0.04 µm final cut-off.

filter_alt

Microchannel-safe purity

0.04 µm final filtration with USP <788> Method 1 particulate compliance supports safe perfusion in microfluidic chip geometries.

science

Specialized dipeptide formulation

L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) replaces free L-glutamine. Use at the same molar concentration as free glutamine (2 mM equivalent). Do not add additional free L-glutamine unless specifically required.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm feed water — low trace-metal and low-TOC formulation background for sensitive cell culture outcomes.

shield

Endotoxin below TLR4 threshold

< 0.05 EU/mL endotoxin release specification — below levels associated with TLR4-mediated inflammatory activation in sensitive immune-cell assays.

balance

HEPES pH stability

25 mM HEPES supplements the sodium bicarbonate buffering system, reducing pH drift during open-bench handling and sampling outside the CO2 incubator.

tune

Customization on demand

pH, concentration, component additions, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

0.04
µm Final pore size — sub-mycoplasma polishing
4
Sequential filtration passes to final cut-off
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMIYAH1X RPMI 1640 Medium with L-Alanyl-Glutamine, 25mM HEPES, 1X Liquid - Quadruple-stage filtration system reaching 0.04 micron final cut-off for organ-on-a-chip and microfluidic cell culture applications - Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMIYAH1X
Applications

Specialized cell culture applications

FluxMPS™ DCP-RPMIYAH1X — RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES — engineered for T cells with 0.04 µm filtration purity for microfluidic and OoC compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request — see the Grade note in the filtration section above.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered RPMI reduces microchannel clogging risk for complex OoC platforms.

OoCMPSToCLoC
Immunology

Immune Cell Culture

RPMI supports T cells, B cells, NK cells, and hematopoietic lines with endotoxin below the TLR4 activation threshold.

T cellsB cellsNK cells
Cell Therapy

CAR-T & TIL Manufacturing

Stable L-Alanyl-L-Glutamine avoids ammonia accumulation over multi-week expansion protocols without mid-process replenishment.

CAR-TTILPBMC
Primary Cells

Primary Cell Culture

Mycoplasma-retentive filtration and < 0.05 EU/mL endotoxin support sensitive primary immune-cell models.

Primary lymphocytesPrimary monocytes
Metabolomics

Metabolic Flux Analysis

Defined formulation base for 13C isotope tracing. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingMetabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal microscopy and biosensor OoC applications. Note: this formulation contains phenol red, which may interfere with certain fluorescence/absorbance-based assays.

ConfocalBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Alanyl-L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 25mM HEPES, [+] Calcium, [+] Magnesium, [+] 2 g/L Glucose, [+] Sodium Pyruvate
Appearance Red-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> 280 – 320 mOsm/kg H2O
Total ingredients 42 components across 4 categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release; see Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 recommended (dual sodium bicarbonate + 25 mM HEPES buffering; HEPES component maintains pH during brief handling outside CO2)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES: 42 ingredients per lot with CAS numbers. L-Alanyl-L-Glutamine (dipeptide, CAS 39537-23-0) replaces free L-glutamine. Use at the same molar concentration as free glutamine (2 mM equivalent). Do not add additional free L-glutamine unless specifically required.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulphate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.00
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Alanyl-L-Glutamine 39537-23-0 0.445
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
Folic Acid 59-30-3 1.000
OTHERS
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Phenol red sodium salt 34487-61-1 5.300
Sodium Pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMIYAH1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm formulation water — low trace-metal, low-TOC background.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280–320 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIYAH1X compares

FluxMPS™ DCP-RPMIYAH1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMIYAH1X (FluxMPS™) Conventional RPMI 1640
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade check_circle Microfluidics Suitable Not applicable Not applicable
RPMI 1640 with L-Alanyl-L-Glutamine + 25mM HEPES — stable nitrogen + pH stability for long-duration T cell / CAR-T culture check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma-retentive filtration check_circle Yes cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Yes cancel Risk of clogging cancel Risk of clogging
Custom formulation Available on request Not typically offered Not typically offered

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIYAH1X — RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES.

Yes. DCP-RPMIYAH1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, supporting low particulate levels for MPS, OoC, and LoC platforms. The RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES formulation is delivered with mycoplasma-retentive filtration and a < 0.05 EU/mL endotoxin release specification for demanding immune-cell culture applications on chip.
FluxMPS™ uses four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration, with mycoplasma-retentive filtration at every stage.
L-Alanyl-L-Glutamine is a stable dipeptide that does not spontaneously degrade during storage the way free L-glutamine does (half-life approximately 3 weeks at 4°C; days at 37°C). It is cleaved by cell-surface dipeptidases to release free glutamine and alanine, providing sustained nitrogen throughout culture. For T cell expansion and CAR-T manufacturing workflows lasting 2–3 weeks, this reduces the need for mid-process glutamine replenishment and the ammonia accumulation associated with free-glutamine breakdown. Combined with 25 mM HEPES for pH stability during open-bench handling and flow cytometry sampling, this base is intended for long-duration immune-cell manufacturing. Use at the same molar concentration as free glutamine (2 mM equivalent); do not add additional free L-glutamine unless specifically required.
5% CO2 is recommended. This formulation is dual-buffered with sodium bicarbonate (2000 mg/L) and 25 mM HEPES (pKa 7.3 at 37°C); the bicarbonate system is calibrated for a 5% CO2 atmosphere, while the HEPES component provides supplemental buffering during open-bench handling and sampling outside the incubator.
Yes. Serum, growth factors, and other protein-containing supplements may be added post-filtration. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane — not 0.04 µm, which retains IgM, VLDL, and lipoprotein fractions and will strip serum of its functional components. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-RPMIYAH1X is manufactured to a release specification of < 0.05 EU/mL, tested per manufacturing batch by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL) prior to release.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 with L-Alanyl-Glutamine + 25mM HEPES in immune-cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120070081012
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Ahmed T. L-alanyl-L-glutamine: a review of its use in clinical nutrition. Nutr Clin Pract. 2001;16:337–348. doi:10.1177/088453360101600605
  6. Christie A, Butler M. Glutamine-based dipeptides are utilized by mammalian cells by extracellular hydrolysis catalyzed by a specific peptidase. J Biotechnol. 1994;37:277–290. doi:10.1016/0168-1656(94)90132-5
  7. Wang FS, et al. Microfluidic technologies for T cell therapy manufacturing. Trends Biotechnol. 2021;39:1131–1144. doi:10.1016/j.tibtech.2021.01.006
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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