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FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-RPMIGH-B1X
$49.50
DCP-RPMIGH-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains High Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-RPMIGH-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered RPMI 1640 formulation engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • High-glucose (4500 mg/L), HEPES-buffered (25 mM) RPMI 1640 formulated without sodium bicarbonate for reduced CO2 dependence
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off, four validated passes
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Contains phenol red as a visual pH indicator; red-colored, clear solution
  • 40 verified ingredients per lot across inorganic salts, amino acids, vitamins, and other components, with CAS traceability
  • Manufactured under an ISO 13485:2016 quality management system with full lot traceability
  • Available in 500 mL and 1000 mL sizes; pH, glucose, HEPES, and nutrient composition customizable on request
CAT. NO.
DCP-RPMIGH-B1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
  • Formulation[+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] High Glucose, [+] Sodium Pyruvate, [-] Sodium Bicarbonate
  • Glucose4500.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
  • CO₂ RequirementHEPES-buffered; reduced CO2 dependence (validate per cell line)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
  • Sizes Available500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes subvisible particulates, mycoplasma-sized organisms, and endotoxin fragments that can confound sensitive cell assays and foul microfluidic channels.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Reduced-particulate media suited to OoC microfluidic chips.

target

Total metabolic control

Defined high-glucose, HEPES-buffered formulation supporting precise control of carbon source and buffering conditions in your experimental system.

water_drop

Ultrapure-grade water

Type 1 water, 18.2 MΩ·cm resistivity. Trace-metal and total organic carbon (TOC) control at the manufacturing source.

visibility

Low background for imaging

Ultra-low particulate baseline from 0.04 µm final filtration supports confocal microscopy, biosensor measurements, and live-cell imaging on-chip.

science

Rich, stable nutrient profile

40 verified ingredients per lot across inorganic salts, amino acids, vitamins, and other components. Full CAS traceability; micro-batch precision manufacturing.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrient composition modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter-plus-final-filter pairs — reaching a final 0.04 µm polish, engineered for applications requiring microchannel-safe purity.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter would not.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for redundant processing.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish; final product QC release gate.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration within the train (not tested per lot); organisms in the mycoplasma size range are approximately 0.2–0.3 µm.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS RPMI 1640 High Glucose 25mM HEPES DCP-RPMIGH-B1X quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 organ-on-a-chip microfluidic cell culture media Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-RPMIGH-B1X.
© Diagnocine® — DCP-RPMIGH-B1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-RPMIGH-B1X delivers Microfluidics Suitable, ultra-filtered purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip applications where reduced-particulate media supports channel integrity.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose RPMI base suited to NCI-60 cancer lines and Warburg effect metabolic studies with defined nutrient control.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered base for iPSC differentiation protocols requiring reduced-particulate media.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Reduced-particulate formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium — this formulation contains phenol red.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate background from 0.04 µm final filtration for confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] High Glucose, [+] Sodium Pyruvate, [-] Sodium Bicarbonate
Appearance Red-colored, clear solution (phenol red indicator present)
Total ingredients 40 components across 4 categories
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement HEPES-buffered; reduced CO2 dependence (validate per cell line)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 4500.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 5.300
Sodium pyruvate 113-24-6 110.000
Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-RPMIGH-B1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity; trace-metal and total organic carbon (TOC) control at the manufacturing source.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to reduce recontamination risk.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

LAL endotoxin test per batch. Release specification: < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate count test. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request: support@diagnocine.com.
Product Comparison

How DCP-RPMIGH-B1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-RPMIGH-B1X (FluxMPS™) Conventional 0.22 µm Filtered Standard Alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation High-glucose, HEPES-buffered, bicarbonate-free Standard RPMI 1640 (bicarbonate-buffered) Standard RPMI 1640 (bicarbonate-buffered)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Type 2 typical Type 2 typical
Manufacturing QMS ISO 13485:2016 ISO 9001 typical Variable
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH-B1X and its use in OoC and MPS applications.

Yes. DCP-RPMIGH-B1X is Microfluidics Suitable and engineered for MPS and OoC platforms. The 0.04 µm final filtration reduces the particulate load that can foul microfluidic channels, making it suited to OoC, ToC, BoC, and LoC applications.
FluxMPS uses a quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2), providing approximately 5× lower particulate counts and 0.1 µm mycoplasma-retentive filtration that standard 0.22 µm filtration does not include.
This RPMI 1640 formulation is HEPES-buffered (25 mM) and formulated without sodium bicarbonate for stable pH control outside a CO2 incubator, such as during open-bench microfluidic handling. If your protocol calls for bicarbonate buffering, use a bicarbonate-containing variant instead of adding sodium bicarbonate to this formulation.
Not strictly. Because this formulation is HEPES-buffered and contains no sodium bicarbonate, it has reduced CO2 dependence and can maintain pH under ambient atmosphere for shorter culture periods. Validate CO2 requirements for your specific cell line and culture duration.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, growth factors, or other additives as required by your cell type and protocol. When sterile-filtering serum-containing or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter; do not use a 0.04 µm filter for serum, as it will retain immunoglobulins and lipoproteins and clog quickly.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by a LAL (Limulus Amebocyte Lysate) assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) for every production batch prior to release.
Yes. A CoA is available per lot upon request at support@diagnocine.com. It includes lot number, expiry, pH, osmolality, endotoxin, sterility, mycoplasma filtration status, USP <788> particulate data, appearance, and full raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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