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FluxMPS™ RPMI 164 Medium w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Product#: DCP-RPMI-QPBR1X
$38.50
DCP-RPMI-QPBR1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium w/o L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate, Phenol Red: 1X Liquid

Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Sodium Bicarbonate Without Phenol Red Without Sodium Pyruvate

FluxMPS™ DCP-RPMI-QPBR1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) RPMI 1640 formulation engineered for hematopoietic cells, lymphocytes and related immune-cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The 0.04 µm final cut-off is five times finer than the 0.22 µm membranes used in conventional sterile filtration. Formulation: [+] Calcium, Magnesium, Glucose (2.0 g/L) | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate, Phenol Red.

  • Glucose is the only added carbon source: 2000 mg/L (2.0 g/L); L-glutamine, sodium pyruvate, sodium bicarbonate and phenol red are not added and must be supplied by the researcher
  • Glutathione (reduced), 1.000 mg/L, included — antioxidant support for redox-sensitive lymphocyte and hematopoietic cell types
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration
  • Endotoxin release specification: < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch
  • Ultrapure Type 1 water (18.2 MΩ·cm) feedstock; ISO 13485:2016 quality management system; ISO Class 5 aseptic fill & finish
  • Bicarbonate-free formulation — CO₂-independent; buffer with HEPES (15–25 mM) as needed for open or closed culture systems
  • Manufactured and released under ISO 13485:2016 QMS with final QC at Diagnocine, Totowa, NJ
CAT. NO.
DCP-RPMI-QPBR1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 — 1X Liquid
  • Media familyRPMI 1640
  • Glucose2000 mg/L (2.0 g/L)
  • Formulation[+] Calcium, Magnesium, Glucose (2.0 g/L) | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate, Phenol Red
  • AppearancePale yellow-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)230–270 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound immune-cell assays. FluxMPS™ is built around a finer filtration train and a source-verified specification matrix.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance — suited to suspension immune-cell OoC and flow cytometry work where particulates confound gating.

science

Immune cell–optimized formulation

RPMI 1640 contains reduced glutathione (antioxidant) and a balanced amino-acid and vitamin profile optimized for lymphocyte and hematopoietic cell culture.

water_drop

Ultrapure-grade water

18.2 MΩ·cm Type 1 feedstock, controlled for trace metals and organic carbon (TOC) — a tighter feed-water specification than purified-water grades.

shield

Below the TLR4 endotoxin threshold

Release specification < 0.05 EU/mL (LAL assay, USP <85>) — formulated for endotoxin-sensitive immune-cell assays where TLR4/NF-κB signaling is a confound.

analytics

Rich nutrient profile

RPMI 1640's specialized amino-acid and vitamin profile is delivered at a controlled particulate baseline for demanding OoC and imaging use.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish, run as two dedicated prefilter + final-filter pairs, finished under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and cell debris; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Mycoplasma-retentive grade (organisms typically 0.2–0.3 µm in diameter); retains sub-micron particulates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Performance vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration of RPMI 1640.

4
Sequential filtration passes to final fill
0.04
µm final pore size
Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma: controlled by 0.1 µm mycoplasma-retentive filtration; not tested per lot.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMI-QPBR1X RPMI 1640 medium Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 for organ-on-a-chip and microfluidic cell culture applications Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-QPBR1X
Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-QPBR1X — RPMI 1640 — delivers 0.04 µm filtered purity for hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant of this formulation, ultra nano-filtered beyond the standard 0.04 µm Microfluidics Suitable line, is available on request for automated bioreactors and robotics.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. FluxMPS™ 0.04 µm filtration limits particulate load that can confound immune assays.

T cellsB cellsNK cellsPBMC
Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and hematopoietic cancer lines where a DMEM background would alter proliferation and signaling.

JurkatRajiK562HL-60
Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph-node-on-chip microphysiological systems (MPS).

Tumor-immune chipLymph node OoCMPS
Immunotherapy

CAR-T & TIL Expansion

Formulated to a < 0.05 EU/mL endotoxin release specification (LAL, USP <85>) to support CAR-T manufacturing and TIL expansion protocols sensitive to endotoxin-driven activation.

CAR-TTILTCR-T
Metabolomics

Immune Cell Metabolic Flux

Bicarbonate-free, phenol red–free base compatible with Agilent Seahorse XF T-cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.

Seahorse XF¹³C tracingGlycolysis
Live-Cell Imaging

Flow Cytometry & Confocal

Phenol red–free formulation with a reduced particulate baseline (0.04 µm final filtration) supports PE-channel flow cytometry and confocal imaging of immune cells.

Flow cytometryConfocalELISA
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Calcium, Magnesium, Glucose (2.0 g/L) | [-] L-Glutamine, Sodium Bicarbonate, Sodium Pyruvate, Phenol Red
Appearance Pale yellow-colored, clear solution
Glucose 2000 mg/L (2.0 g/L)
L-Glutamine Not added / None
Sodium Pyruvate Not added / None
Sodium Bicarbonate Not added / None
Phenol Red Not added / None
pH USP <791> 7.4
Osmolality USP <785> 230–270 mOsm/kg H₂O
Total ingredients 37
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec; see § Quality Assurance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent (bicarbonate-free); supplement with HEPES (15–25 mM) for pH buffering
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Available pack sizes 500 mL, 1000 mL
Formulation

Full composition (mg/L)

RPMI 1640: 37 ingredients across 4 categories, verified per lot with CAS numbers for raw-material traceability. Contains reduced glutathione and a balanced amino-acid/vitamin profile optimized for lymphocyte and hematopoietic cell culture.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6000.000
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.00
Pyridoxine hydrochloride 58-56-0 1.00
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 0.005
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
i-Inositol 87-89-8 35.000
D-Glucose 50-99-7 2000.000
Glutathione reduced 70-18-8 1.000
Custom formulation: Contact support@diagnocine.com for DCP-RPMI-QPBR1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016–certified quality management system. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm Type 1 water, controlled for trace metals and organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 230–270 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMI-QPBR1X compares

FluxMPS™ DCP-RPMI-QPBR1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter DCP-RPMI-QPBR1X (FluxMPS™) Conventional RPMI 1640
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
Formulation trait Bicarbonate-free, phenol red-free, CO₂-independent (HEPES-bufferable); all secondary carbon/nitrogen researcher-defined cancel Typically bicarbonate-buffered cancel Typically bicarbonate-buffered
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm stage) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-QPBR1X — RPMI 1640.

Yes. DCP-RPMI-QPBR1X is Microfluidics Suitable, processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size. RPMI 1640 with mycoplasma-retentive filtration is suited to immune-cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models where standard RPMI carries a higher particulate load.
FluxMPS™ runs four sequential filters — 0.1 µm prefiltration I, 0.04 µm final filtration I (mycoplasma-retentive grade), 0.1 µm prefiltration II, and 0.04 µm final filtration II (polish) — reaching a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used in conventional sterile filtration.
All optional components are removed so the researcher fully controls the culture system: bicarbonate-free (CO₂-independent, HEPES-bufferable), phenol red-free, with all secondary nitrogen and carbon inputs left undefined. Only the RPMI 1640 salt/vitamin/amino-acid scaffold and 2 g/L glucose remain. Add L-glutamine (or a stable dipeptide substitute), sodium pyruvate, sodium bicarbonate, phenol red and/or HEPES as your assay requires.
No sodium bicarbonate is added, so this formulation is CO₂-independent. Supplement with HEPES (15–25 mM) for pH buffering in open or ambient-air culture systems.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before use; do not use a 0.04 µm filter for serum, which will strip lipoproteins and clog rapidly. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch; every batch is tested before release and must meet a release specification of < 0.05 EU/mL by LAL assay (USP <85>). This matters for immune-cell assays, where endotoxin can activate TLR4/NF-κB signaling independently of experimental conditions.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune-cell culture and OoC applications.

  1. Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1001/jama.1967.03120080053007
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Rothbauer M, et al. Recent advances in microfluidic technologies for cell-to-cell interaction studies. Lab Chip. 2018;18:249–270. doi:10.1039/c7lc00815e
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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