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FluxMPS™ Penicillin-Streptomycin (10,000 U/mL)

Product#: DCP-PCSM100X
$31.71
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
FluxMPS™ Cell Culture Supplements
ISO 13485 Certified Manufacturing

FluxMPS™ Penicillin-Streptomycin (10,000 U/mL)

Contains Penicillin (10,000 U/mL) Contains Streptomycin (10,000 µg/mL)

DCP-PCSM100X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 100X liquid antibiotic concentrate supplying 10,000 U/mL penicillin and 10,000 µg/mL streptomycin. It is formulated as a bacterial-contamination-control supplement for mammalian and insect cell culture media, sterile-filtered to a 0.04 µm final cut-off for compatibility with microfluidic channels and organ-on-a-chip (OoC) perfusion systems.

  • 100X concentrate: 10,000 U/mL penicillin + 10,000 µg/mL streptomycin; add at typical working concentrations of 50-100 U/mL penicillin and 50-100 µg/mL streptomycin
  • Sterile-filtered through a quadruple-stage 0.1 µm ×2 + 0.04 µm ×2 filtration train
  • Dual-mechanism, broad-spectrum activity against most Gram-positive and Gram-negative contaminants (bacterial cell-wall synthesis inhibition + 30S ribosomal subunit binding)
  • Validated for use in mammalian and insect cell culture systems
  • Liquid, ready-to-add format; store at -20°C, protect from light, avoid repeated freeze-thaw cycles
  • 12-month shelf life from date of manufacture, unopened
  • Manufactured under an ISO 13485:2016 quality management system
  • Custom concentrations, pH, and additive formulations available on request — contact support@diagnocine.com
SKU: DCP-PCSM100X Cell Culture Antibiotics & Supplements UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Penicillin-Streptomycin (10,000 U/mL) — 100X Liquid
  • Concentration100X
  • Penicillin10,000 U/mL
  • Streptomycin10,000 µg/mL
  • Working Concentration50-100 U/mL / 50-100 µg/mL
  • Culture TypeMammalian & Insect Cell Culture
  • ApplicationPrevention of Cell Culture Contamination
  • FiltrationQuadruple-stage (0.1 µm ×2 + 0.04 µm ×2)
  • Storage-20°C; protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingDry Ice
ISO 13485:2016 Quadruple-Stage 0.04 µm Filtration RUO
Why FluxMPS™

Engineered where standard antibiotic supplements fall short

Conventional 0.22 µm-filtered antibiotic solutions can carry sub-micron particulates and mycoplasma-scale organisms into sensitive microfluidic and organ-on-a-chip (OoC) systems. DCP-PCSM100X is processed through Diagnocine's quadruple-stage filtration train to reduce that risk while delivering reliable, broad-spectrum contamination control.

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Microchannel-safe purity

A 0.1 µm + 0.04 µm prefilter/final-filter pair, run twice in series, reduces sub-micron particulate load before the antibiotic concentrate reaches a microfluidic channel.

shield

Broad-spectrum contamination control

Penicillin and streptomycin act on complementary bacterial targets, giving coverage against most Gram-positive and Gram-negative contaminants commonly introduced during routine culture handling.

science

Dual mechanism of action

Penicillin inactivates penicillin-binding proteins and disrupts bacterial cell-wall synthesis; streptomycin binds the bacterial 30S ribosomal subunit and halts protein synthesis, regardless of division state.[1]

biotech

Validated across culture systems

Formulated for use as a supplement in mammalian and insect cell culture media, including primary cells and T-cell culture systems.

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Rigorous batch processing

Each batch is sterile-filtered through the quadruple-stage train and manufactured under an ISO 13485:2016 quality management system prior to release.

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Customization on demand

Alternate concentrations, pH, or the addition of other chemicals, compounds, proteins, or supplements can be produced on request.

Purity Architecture

Quadruple-stage filtration system

DCP-PCSM100X is processed through a validated four-pass filtration train — two dedicated prefilter/final-filter pairs run in series — reaching a 0.04 µm final pore size before aseptic fill.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulate and protein aggregate load, protecting the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates that pass a conventional 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm final-filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill and finish.

Filtration architecture

The train is a repeated prefilter + final-filter pair, not a single descending cascade: each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, and the sequence runs twice in series for full redundancy.

0.04 µmFinal pore size
4Total filtration passes
This product is sterile-filtered using Diagnocine's validated quadruple-stage (0.1 µm ×2 + 0.04 µm ×2) process described above.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-PCSM100X Penicillin-Streptomycin 10,000 U/mL quadruple-stage 0.1 micron x2 plus 0.04 micron x2 filtration system diagram for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) applied to DCP-PCSM100X.
© Diagnocine® — DCP-PCSM100X
Applications

Where DCP-PCSM100X is used

As a supplement added to complete cell culture media, DCP-PCSM100X controls bacterial contamination across a range of mammalian and insect cell culture systems — while requiring the same caution any Pen/Strep supplement demands regarding off-target cellular effects.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For closed-loop automated bioreactor and liquid-handling robotics platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this antibiotic concentrate, engineered for systems where sub-40 nm particulate exclusion protects narrow-bore tubing, valves, and inline sensors.

  • Total Particulate Exclusion — six-stage cascade down to a 0.01 µm final cut-off
  • Valve & Sensor Protection — reduced fouling risk in automated perfusion hardware
  • Extended Perfusion Stability — supports longer unattended run times

Inquiry Required: the 0.01 µm MPS Grade variant is produced on request — contact support@diagnocine.com to discuss requirements.

Cell Culture

Mammalian & Insect Cell Systems

General-purpose bacterial contamination control for routine mammalian and insect cell culture maintenance.

Mammalian cellsSf9/Sf21 insect cells
Immunology

T-Cell & Primary Cell Culture

Used to help control contamination risk in primary cell and T-cell culture workflows.

Primary cellsT-cells
Stem Cell Biology

Use With Caution — Differentiation Studies

Antibiotics can affect proliferation and differentiation potential in stem cell cultures; monitor for confounding effects before adopting for definitive differentiation studies.

Stem cellsDifferentiation assays
Cancer Biology

Melanoma & Cancer Cell Lines

Reported to moderately stimulate melanogenic enzymes (dopa oxidase, tyrosine hydroxylase) in melanoma cultures over 24-48 hours; account for this in pigmentation-related endpoints.

Melanoma cell linesPigmentation assays
Dermatology / Tissue Models

Keratinocyte & 3D Skin Models

Can reduce proliferation of normal human epidermal keratinocytes (NHEK) and hinder full epidermal differentiation in 3D skin models; use judiciously in these systems.

NHEK3D skin models
Toxicogenomics

Gene Expression & Chromatin Studies

Genome-wide studies in human HepG2 liver cells report Pen/Strep-induced changes in gene expression and chromatin landscape across drug-response, insulin-response, fatty-acid metabolism, apoptosis, cell-growth, and unfolded-protein-response pathways.[1]

HepG2Gene expression
Technical Specifications

Formulation, safety, and logistics parameters

Specifications reflect data provided in Diagnocine's source formulation record. Parameters not stated in that record (endotoxin, osmolality, CO₂ requirement, mycoplasma testing, sodium bicarbonate) are intentionally omitted rather than estimated.

Physical & Formulation Parameters
Parameter Specification
Active Ingredient 1 Penicillin, 10,000 U/mL
Active Ingredient 2 Streptomycin, 10,000 µg/mL
Concentration 100X
Working Concentration 50-100 U/mL penicillin / 50-100 µg/mL streptomycin
Form Liquid
Product Type Antibiotic supplement
Sterility & Filtration Parameters
Parameter Specification
Sterility Sterile-filtered Filtration-based
Filtration System Quadruple-stage: 0.1 µm ×2 + 0.04 µm ×2
Culture Type Mammalian Cell Culture, Insect Cell Culture
Application Prevention of cell culture bacterial contamination
Storage, Handling & Logistics
Parameter Specification
Storage Temperature -20°C
Light Protection Protect from light
Freeze-Thaw Repeated freezing and thawing should be avoided
Shelf Life 12 months from date of manufacture, unopened
Shipping Condition Dry ice
Raw Materials & Regulatory Traceability
Parameter Specification
Manufacturing QMS ISO 13485:2016
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment Research Use Only (RUO)
Grade Microfluidics Suitable (0.04 µm final cut-off)
Intended Use Research Use Only. Not for clinical, diagnostic, or therapeutic use in humans
Formulation

Full composition

DCP-PCSM100X is a two-component antibiotic concentrate rather than a multi-salt basal medium; its active ingredients, formulation details, and recommended working concentrations are summarized below.

ACTIVE INGREDIENTS
Component CAS Number Concentration
Penicillin G (sodium salt) 69-57-8 10,000 U/mL
Streptomycin sulfate 3810-74-0 10,000 µg/mL
FORMULATION DETAILS
Attribute CAS Number Value
Concentration (stock) — 100X
Form — Liquid
Culture Type Compatibility — Mammalian Cell Culture, Insect Cell Culture
Product Type — Antibiotics
RECOMMENDED WORKING CONCENTRATIONS
Component CAS Number Typical Working Concentration
Penicillin 69-57-8 50-100 U/mL
Streptomycin 3810-74-0 50-100 µg/mL
Alternate concentrations, additions of other chemicals/compounds/proteins/supplements, and modified formulations are available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & batch quality control

DCP-PCSM100X is manufactured under Diagnocine's ISO 13485:2016 quality management system, with quadruple-stage filtration and per-batch release testing applied prior to shipment.

verified

ISO 13485:2016 QMS

Manufactured under a certified ISO 13485:2016 quality management system.

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Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 sterile filtration train applied to every batch.

fact_check

Per-Lot QC & CoA

Each lot is released against internal quality criteria, with a Certificate of Analysis available on request.

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Finished in Totowa, NJ

Final quality control and release performed at Diagnocine's Totowa, NJ facility.

Sterility

Sterile-filtered via the quadruple-stage 0.1 µm ×2 + 0.04 µm ×2 process described above.

Filtration Process

Two dedicated prefilter (0.1 µm) + final-filter (0.04 µm) pairs run in series.

Storage Stability

Store at -20°C, protect from light; repeated freeze-thaw cycles should be avoided to preserve potency.

Documentation / CoA

A Certificate of Analysis documenting lot number and manufacture date is available on request.

Batch-level quality control. Each batch of DCP-PCSM100X is manufactured under Diagnocine's ISO 13485:2016 quality management system and undergoes sterility testing and quadruple-stage filtration validation prior to release. A Certificate of Analysis is available on request from support@diagnocine.com.
Product Comparison

How DCP-PCSM100X compares

Comparison against conventional single-stage 0.22 µm-filtered Pen/Strep antibiotic supplements commonly used in cell culture.

Parameter DCP-PCSM100X (FluxMPS™) Conventional Pen/Strep (0.22 µm filtered) Standard Antibiotic-Antimycotic Solution (0.22 µm filtered)
Grade Microfluidics Suitable Standard grade Standard grade
Filtration stages 4 (0.1 µm ×2 + 0.04 µm ×2) 1 (0.22 µm single-stage) 1 (0.22 µm single-stage)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Antimicrobial spectrum Penicillin + Streptomycin (broad-spectrum Gram+/Gram-) Penicillin + Streptomycin Penicillin + Streptomycin + Amphotericin B
Endotoxin (release specification) Not specified for this product Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Culture type compatibility Mammalian & Insect Cell Culture Mammalian Mammalian
Custom formulation check_circle cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about using DCP-PCSM100X in cell culture and microfluidic workflows.

Yes. DCP-PCSM100X is filtered through Diagnocine's quadruple-stage (0.1 µm ×2 + 0.04 µm ×2) system, reducing sub-micron particulate load relevant to narrow microfluidic channels and OoC perfusion systems. As with any antibiotic supplement, researchers should confirm it does not confound their specific assay readouts before routine use in a chip-based system.
Standard antibiotic supplements are typically passed through a single 0.22 µm sterilizing-grade filter. DCP-PCSM100X instead runs two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs in series, four passes in total, reducing sub-micron particulate carryover beyond what a single 0.22 µm pass achieves.
Published studies show Penicillin-Streptomycin can induce global gene expression and chromatin changes in human cell lines such as HepG2, affecting pathways including drug response, insulin response, fatty acid metabolism, apoptosis, cell growth, and the unfolded protein response.[1] It has also been shown to moderately stimulate melanogenic enzymes in melanoma cultures and to reduce proliferation and differentiation capacity in normal human epidermal keratinocytes and 3D skin models. Researchers working with stem cells, primary cancer cells, keratinocytes, or transcriptomic endpoints should run antibiotic-free control cultures where feasible and weigh contamination prevention against these potential confounds.
Yes. Store DCP-PCSM100X at -20°C, protected from light, and avoid repeated freeze-thaw cycles, which can reduce antibiotic potency over time. Aliquoting on first thaw is recommended for frequent users.
Yes, it is typically added alongside serum and other supplements at the point of complete-media preparation. If a serum or protein-containing addition requires post-hoc filtration, use a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for serum or protein-containing additions, as it will retain immunoglobulins, lipoproteins, and other serum components.
Each batch is manufactured under Diagnocine's ISO 13485:2016 quality management system and is sterile-filtered through the quadruple-stage (0.1 µm ×2 + 0.04 µm ×2) process prior to release. Diagnocine has not published a specific endotoxin release specification for this product; contact support@diagnocine.com for the current Certificate of Analysis and available batch documentation.
Yes. A Certificate of Analysis is available on request and documents lot number, manufacture date, and applicable release testing performed for that batch. Contact support@diagnocine.com to request a CoA for a specific lot.
Scientific References

Supporting literature

Citations and curated literature relevant to antibiotic use in cell culture, mycoplasma control, and microfluidic/organ-on-a-chip applications.

Citations

  1. Ryu AH, Eckalbar WL, Kreimer A, Yosef N, Ahituv N. Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulation. Sci Rep. 2017;7(1):7533. doi:10.1038/s41598-017-07757-w

Supporting Literature

  1. Uphoff CC, Drexler HG. Detection of Mycoplasma in cell cultures. Curr Protoc Mol Biol. 2014;106:24.4.1-24.4.14. doi:10.1002/0471142727.mb2404s106
  2. Nims RW, Price PJ. Best practices for detecting and mitigating mycoplasma contamination in cell culture. J Vis Exp. 2017. doi:10.3791/57127
  3. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361. doi:10.1038/s41573-020-0079-3
  4. Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017;17(14):2395-2420. doi:10.1039/c6lc01554a
  5. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
  7. Skardal A, Shupe T, Atala A. Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling. Drug Discov Today. 2016;21(9):1399-1411. doi:10.1016/j.drudis.2016.07.003
  8. Coecke S, Balls M, Bowe G, et al. Guidance on good cell culture practice: a report of the second ECVAM task force. Altern Lab Anim. 2005;33(3):261-287. doi:10.1177/026119290503300313
  9. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 7th ed. Hoboken, NJ: Wiley; 2016.

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