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FluxMPS™ Nutrient Mixture F-12 Ham, Coon’s Modification w/ Sodium bicarbonate 1X Liquid Cell Culture Medium
FluxMPS™ Nutrient Mixture F-12 Ham, Coon’s Modification w/ Sodium bicarbonate 1X Liquid Cell Culture Medium
FluxMPS™ DCP-F12HCM1X is a Microfluidics Suitable, ultra-filtered Nutrient Mixture F-12 Ham, Coon’s Modification formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: L-Glutamine, Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, D-Glucose (2.0 g/L), Sodium Pyruvate.
- Ham’s F-12 Coon’s Modification with elevated amino acids, sodium pyruvate, and added L-ascorbic acid for demanding hybrid-cell fusion and CHO culture protocols
- Formulation: L-Glutamine, Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, D-Glucose (2.0 g/L), Sodium Pyruvate
- Quadruple-stage sequential filtration — 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — reaching a 0.04 µm final pore size
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Sodium bicarbonate–buffered (2676 mg/L); CO₂ requirement derived from bicarbonate concentration — approximately 7% CO₂ to maintain pH 7.4
- Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
- 50 verified ingredients across four composition categories with full lot traceability
- Custom pH, salts, and nutrient adjustments available on request
- FormulationL-Glutamine, Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, D-Glucose (2.0 g/L), Sodium Pyruvate
- AppearancePink-Red colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Total ingredients50
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and endotoxin-bearing debris that clog microfluidic channels and corrupt sensor signals. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion across common chip geometries.
Total metabolic control
Selective inclusion of glutamine, pyruvate, and sodium bicarbonate for precise nutrient and buffer definition.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) for controlled trace-metal and organic-carbon (TOC) background.
Low background for imaging
Ultra-low particulate baseline from 0.04 µm final filtration reduces background scatter for confocal microscopy and optical biosensor platforms.
Rich, stable nutrient profile
50 ingredients verified per lot; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages, run as two dedicated prefilter + final-filter pairs, reach a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-grade particulate control beyond what conventional 0.22 µm filtration achieves.
-
1
0.1 µm Prefiltration I — Large Particulate Removal
Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a standard 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm cartridge, providing full redundancy across the train.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter prior to aseptic fill and finish under ISO Class 5 (Class 100) conditions.
Performance vs. conventional media
© Diagnocine® — DCP-F12HCM1X
Designed for next-generation cell models
FluxMPS™ DCP-F12HCM1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems, as well as classical hybrid-cell and CHO culture work.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling risk for solenoid valves and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
Ultra-clean 0.04 µm–filtered media reduces microchannel clogging risk in complex multi-organ chip architectures.
CHO & Mammalian Cell Culture
Elevated amino acid and sodium pyruvate levels support CHO, hybrid, and primary cell clonal growth protocols.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and 0.1 µm mycoplasma-retentive filtration support sensitive iPSC-derived protocols.
Endothelial & Primary Cells
Particulate-controlled, endotoxin-specified media for HUVEC monolayer integrity and TEER monitoring.
Metabolic Flux Analysis
Defined, elevated-pyruvate formulation provides a consistent background for 13C isotope tracing and NMR-based metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline from 0.04 µm final filtration supports confocal microscopy and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | L-Glutamine, Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, D-Glucose (2.0 g/L), Sodium Pyruvate |
| Appearance | Pink-Red colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Glucose | 2.0 g/L (2000 mg/L) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | 220 mg/L |
| Phenol Red | 1.240 mg/L (phenol red sodium salt) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 7% CO₂ (derived from 2676 mg/L sodium bicarbonate to maintain pH 7.4) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
50 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham’s F-12 Coon’s Modification carries zinc, putrescine, hypoxanthine and thymidine in addition to standard amino acids and vitamins, with doubled amino acid and pyruvate levels plus added ascorbic acid versus standard Ham’s F-12.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 165.000 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 132.040 |
| Ferric sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride hexahydrate | 7791-18-6 | 106.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 50.800 |
| Potassium chloride | 7447-40-7 | 305.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 68.000 |
| Sodium bicarbonate | 144-55-8 | 2676.000 |
| Sodium chloride | 7647-14-5 | 7530.000 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.144 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 16.000 |
| L-Alanine | 56-41-7 | 18.000 |
| L-Arginine hydrochloride | 1119-34-2 | 422.000 |
| L-Asparagine anhydrous | 70-47-3 | 30.000 |
| L-Aspartic acid | 56-84-8 | 26.000 |
| L-Cysteine hydrochloride | 52-89-1 | 70.260 |
| L-Glutamic acid | 56-86-0 | 30.000 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 7.800 |
| L-Leucine | 61-90-5 | 26.200 |
| L-Lysine hydrochloride | 657-27-2 | 73.000 |
| L-Methionine | 63-68-3 | 9.000 |
| L-Phenylalanine | 63-91-2 | 10.000 |
| L-Proline | 147-85-3 | 70.000 |
| L-Serine | 56-45-1 | 21.000 |
| L-Threonine | 72-19-5 | 23.800 |
| L-Tryptophan | 73-22-3 | 4.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 15.860 |
| L-Valine | 72-18-4 | 23.400 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Folic acid | 59-30-3 | 1.320 |
| L-Ascorbic acid | 50-81-7 | 45.000 |
| Nicotinamide | 98-92-0 | 0.040 |
| Pyridoxine hydrochloride | 58-56-0 | 0.060 |
| Riboflavin | 83-88-5 | 0.040 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| i-Inositol | 87-89-8 | 18.020 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Hypoxanthine sodium salt | 45738-97-4 | 4.040 |
| Linoleic acid | 60-33-3 | 0.090 |
| Lipoic acid | 1077-28-7 | 0.206 |
| Phenol red sodium salt | 34487-61-1 | 1.240 |
| Putrescine dihydrochloride | 333-93-7 | 0.300 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| Thymidine | 50-89-5 | 0.700 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016 quality management system. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity Type 1 water, controlled for trace metals and organic carbon (TOC).
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-F12HCM1X compares
FluxMPS™ DCP-F12HCM1X vs. conventional 0.22 µm–filtered Ham’s F-12 Coon’s Modification formulations.
| Parameter | DCP-F12HCM1X (FluxMPS™) | Conventional Ham’s F-12 Coon’s Mo (0.22 µm filtered) |
Standard Alt. (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final) | Not graded (0.22 µm filtered) | Not graded (0.22 µm filtered) |
| Coon’s Modification — doubled amino acids and pyruvate plus ascorbic acid for hybrid cell culture | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration (0.1 µm) | check_circle Yes | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-F12HCM1X.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research, and the underlying Ham’s F-12 Coon’s Modification formulation.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG, McKeehan WL. Development of improved media and culture conditions for clonal growth of normal diploid cells. In Vitro. 1978;14:11–22. doi:10.1007/BF02618171
- Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids. Proc Natl Acad Sci USA. 1969;62:852–859. doi:10.1073/pnas.62.3.852
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j