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FluxMPS™ Minimum Essential Medium (MEM) w/o Sodium Pyruvate: 2X Liquid

Product#: DCP-MEM-PN2X
$63.80
DCP-MEM-PN2X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM) w/o Sodium Pyruvate: 2X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without Sodium Pyruvate

FluxMPS™ DCP-MEM-PN2X is a Microfluidics Suitable, ultra-filtered MEM 2X without Sodium Pyruvate and NEAA formulation on an Earle's Salts base, engineered for primary fibroblasts and related cell models used on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. It is processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, well below the 0.22 µm industry standard. This is a 2X concentrated formulation — dilute 1:1 with sterile water, cell suspension, or supplements to reach 1X working concentration. Sodium pyruvate and NEAA are intentionally excluded so researchers can define these components independently at final 1X concentration.

  • MEM 2X without Sodium Pyruvate and NEAA on an Earle's Salts base — a 2X concentrated formulation for 1:1 dilution
  • Glucose: 2000 mg/L (2.0 g/L, 2X concentrated; 1.0 g/L at 1X working concentration)
  • L-Glutamine: 584 mg/L (2X concentrated); Sodium Bicarbonate: 4400 mg/L (2X concentrated)
  • Sodium pyruvate and NEAA intentionally omitted — add independently at final 1X concentration to avoid double-dosing
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, concentration, and component modifications available on request
CAT. NO.
DCP-MEM-PN2X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Minimum Essential Medium (MEM) w/o Sodium Pyruvate: 2X Liquid
  • Glucose2000 mg/L (2.0 g/L, 2X concentrated)
  • L-Glutamine584 mg/L (2X concentrated)
  • Sodium PyruvateNone / Not added (excluded from formulation)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack, 2–8°C maintained in transit
Available pack sizes: 500 mL, 1000 mL.
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered MEM passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound sensitive cell culture applications. FluxMPS™ addresses these failure modes with a four-stage filtration train reaching a 0.04 µm final cut-off.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 (light obscuration) particulate testing support safe perfusion in microfluidic chip geometries.

target

Defined dilution control

Supplied as a 2X concentrate on an Earle's Salts base with 2 g/L glucose and 584 mg/L L-glutamine. Sodium pyruvate and NEAA are withheld so researchers set these components independently at the final 1X working concentration.

water_drop

Ultrapure-grade water

Manufactured with Type 1 water (18.2 MΩ·cm), supporting trace-metal and organic-carbon control in the finished medium.

visibility

Low background for imaging

Quadruple-stage filtration to 0.04 µm keeps particulate baseline low for confocal and biosensor-based readouts. This formulation contains phenol red; phenol-red-free variants are available on request for optical/TEER applications sensitive to indicator dye.

science

Complete amino acid & vitamin profile

Full MEM amino acid and B-vitamin complement, released per manufacturing batch with full CAS-level traceability.

tune

Customization on demand

pH, concentration factor, component additions, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four filtration passes — two dedicated prefilter/final-filter pairs — reaching a final 0.04 µm polish.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris and protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second, dedicated prefilter protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish (ISO Class 5).

Performance vs. conventional media

The 0.04 µm final cut-off of DCP-MEM-PN2X falls well below the 0.22 µm pore size used in conventional filtered media.

4
Filtration passes (2 prefilter + 2 final-filter stages)
0.04
µm final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms are typically 0.2–0.3 µm in diameter.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-MEM-PN2X Minimum Essential Medium (MEM) w/o Sodium Pyruvate 2X Liquid, Quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 final polish, Microfluidics Suitable for organ-on-a-chip and OoC applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-MEM-PN2X
Applications

Specialized cell culture applications

FluxMPS™ DCP-MEM-PN2X — MEM 2X without Sodium Pyruvate and NEAA — engineered for primary fibroblasts and related models, with 0.04 µm filtration purity for microfluidic compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM 2X helps prevent microchannel clogging in complex OoC platforms.

OoCMPSToCLoC
Primary Cells

Primary Cell Culture

Mycoplasma-retentive filtration and a < 0.05 EU/mL endotoxin release specification support sensitive primary cell models.

Primary fibroblastsPrimary epithelial cellsPrimary neurons
Metabolomics

Metabolic Flux Analysis

2X concentrate base for researcher-defined pyruvate/NEAA supplementation, suited to ¹³C isotope tracing studies.

¹³C tracingMetabolomics
Vascular Biology

Endothelial & Primary Cells

Sub-mycoplasma filtration purity supports co-culture models with endothelial and stromal cell types.

HUVECsPrimary fibroblasts
Stem Cell Biology

iPSC-Derived Models

Defined 2X base compatible with iPSC-derived fibroblast and mesenchymal models requiring precise nutrient control.

iPSC-FibroblastsiPSC-MSC
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate baseline from Quadruple-stage filtration supports confocal and biosensor-based OoC readouts. Contains phenol red; a phenol-red-free variant is available on request for TEER applications.

ConfocalBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose | [-] Sodium Pyruvate (on an Earle's Salts base, NEAA also excluded)
Appearance Orange-to-red colored, clear solution
Glucose 2000 mg/L (2.0 g/L, 2X concentrated)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Total ingredients 29 total, grouped into 4 composition categories (Inorganic Salts, Amino Acids, Vitamins, Others) shown across 3 tabs
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack, 2–8°C maintained in transit
CO2 requirement ~5% CO2 recommended (derived from ~26 mM NaHCO3 at 1X working concentration)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM 2X without Sodium Pyruvate and NEAA: 29 ingredients verified per manufacturing batch with CAS numbers. This is a 2X concentrated formulation — dilute 1:1 with sterile water, cell suspension, or supplements to reach 1X working concentration. Sodium pyruvate and NEAA are absent and should be added independently at desired final concentration after dilution.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 530.000
Magnesium sulfate anhydrous 7487-88-9 195.44
Potassium chloride 7447-40-7 800.000
Sodium bicarbonate 144-55-8 4400.00
Sodium chloride 7647-14-5 13600.000
Sodium phosphate dibasic anhydrous 7558-79-4 244.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 252.000
L-Cystine dihydrochloride 30925-07-6 62.600
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 84.000
L-Isoleucine 73-32-5 104.000
L-Leucine 61-90-5 104.000
L-Lysine hydrochloride 657-27-2 145.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 64.000
L-Threonine 72-19-5 96.000
L-Tryptophan 73-22-3 20.000
L-Tyrosine disodium salt 122666-87-9 103.800
L-Valine 72-18-4 92.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Niacinamide 98-92-0 2.000
Pyridoxine hydrochloride 58-56-0 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
OTHERS
D-Glucose 50-99-7 2000.000
Phenol red sodium salt 34487-61-1 22.000
i-Inositol 87-89-8 4.000
Custom formulation: Contact support@diagnocine.com for DCP-MEM-PN2X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QC at Diagnocine R&D Center, Totowa, NJ, USA.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train reaching a 0.04 µm final polish.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Per-Lot QC & CoA

Small-batch, per-lot tested — Certificate of Analysis available for every batch.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL, tested per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration particle count: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-PN2X compares

FluxMPS™ DCP-MEM-PN2X vs. conventional 0.22 µm–filtered MEM formulations.

Parameter DCP-MEM-PN2X (FluxMPS™) Conventional MEM 2X
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable Standard Standard
MEM 2X without Sodium Pyruvate and NEAA — double-concentrated base for dilution with researcher-defined pyruvate and NEAA check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple-stage) 1 1
Mycoplasma barrier filtration check_circle 0.1 µm retentive cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-PN2X — MEM 2X without Sodium Pyruvate and NEAA.

Yes. DCP-MEM-PN2X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, giving a low particulate baseline suited to MPS, OoC and LoC platforms. As a 2X concentrated MEM without sodium pyruvate and NEAA, it is well suited to primary fibroblast and related cell models where researchers want independent control over pyruvate/NEAA supplementation.
FluxMPS™ uses four sequential filtration passes — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II (Polish) — reaching a 0.04 µm final cut-off, well below the 0.22 µm pore size of conventional filtered media.
DCP-MEM-PN2X is a double-concentration (2X) formulation designed for 1:1 dilution with an equal volume of cell suspension, conditioned medium, or supplements, yielding 1X MEM at final working concentration. Sodium pyruvate and NEAA are excluded so researchers can add them independently at the final 1X concentration, avoiding double-dosing when they are already present in the diluent volume. Note that L-glutamine (584 mg/L, 2X) and D-glucose (2000 mg/L, 2X) are already present in this formulation.
This formulation contains sodium bicarbonate (4400 mg/L, 2X concentrated; approximately 26 mM NaHCO3 at 1X working concentration). Based on this bicarbonate concentration, approximately 5% CO2 is recommended to maintain pH 7.4; confirm against your own incubator calibration.
Yes. Add FBS, serum-free supplements, growth factors, sodium pyruvate, NEAA, or antibiotics as required at final 1X concentration after dilution. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — never a 0.04 µm membrane, which will retain serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulation.
Diagnocine's release specification for DCP-MEM-PN2X is < 0.05 EU/mL, verified by LAL assay (USP <85>) per manufacturing batch, not per individual unit. See the Product Comparison section for how this compares to other suppliers' published specifications.
Yes. Full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma control status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM formulations and microfluidic/OoC cell culture applications.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  4. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  5. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  6. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  7. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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