FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Phenol Red: 1X Liquid
FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o Phenol Red: 1X Liquid
FluxMPS™ DCP-MEM-R1X is an MPS-grade, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Phenol Red.
- MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
- NEAA included reduces metabolic burden on primary cells
- Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
- 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin for sensitive primary cell cultures
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom formulation modifications available on request
- BaseMEM + Earle’s Salts + NEAA
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- Formulation[+] Earle's Salts, [+] NEAA, [+] Low Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate
[-] Phenol Red - AppearancePale Yellow-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)290.00–330.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months / 2–8°C
Engineered for primary cell culture where standard media fails
MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma, subvisible particulates, and endotoxin fragments that cause subtle but significant alterations to primary cell gene expression, activation state, and morphology. FluxMPS™ eliminates these failure modes while preserving the full MEM + NEAA nutritional profile that primary cells depend on.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in neuronal, epithelial, and fibroblast chip architectures.
Primary cell–optimized formulation
MEM + Earle’s Salts + NEAA: the established standard for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) — eliminates ionic contaminants that alter primary cell ion channel activity, TEER measurements, and electrophysiology.
Below TLR4 endotoxin threshold
< 0.05 EU/mL endotoxin — prevents LPS-driven inflammatory activation that confounds primary cell phenotype, fibroblast-to-myofibroblast transition, and neuronal inflammatory responses.
NEAA reduces metabolic burden
Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, improving viability in low-serum conditions and reducing ammonia accumulation from NEAA synthesis pathways.
Customization on demand
pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions. For primary cell culture, sub-mycoplasma filtration is especially critical — mycoplasma in MEM causes altered cytokine profiles, metabolic shifts, and gene expression changes that can persist undetected for months.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries from fouling.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration. Critical for primary cell cultures where mycoplasma causes undetected phenotypic drift.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional MEM
FluxMPS™ DCP-MEM-R1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered MEM, with a confirmed mycoplasma barrier at every production stage — essential for long-duration primary cell OoC experiments.
© Diagnocine® — DCP-MEM-R1X
Primary cell models & OoC applications
FluxMPS™ DCP-MEM-R1X is purpose-built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available for automated bioreactor perfusion of primary cell cultures where trace particulates cause accelerated chip fouling.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
- Valve & Sensor Protection: Eliminates micro-fouling in delicate neuronal and epithelial chip geometries
- Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Neuronal Chips & Brain-on-Chip
MEM + Earle’s Salts + NEAA + low glucose is the physiological-closest base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.
Epithelium-on-Chip
Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and their barrier integrity in transwell and microfluidic TEER-monitored platforms.
Primary Fibroblast & Stromal Models
MEM with NEAA is the classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation that promotes myofibroblast differentiation artefacts.
Vascular Cell Culture
Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.
Organ-on-a-Chip & MPS
0.04 µm filtered MEM prevents particulate clogging in sub-100 µm neuronal and epithelial chip microchannels where standard MEM would cause progressive flow reduction.
Microscopy & Optical Sensing
Ultra-low particulate MEM; phenol red–free variants eliminate autofluorescence for confocal, TEER sensor, and biosensor applications on primary cell chips.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Earle's Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Phenol Red |
| Appearance | Pale Yellow-colored, clear solution |
| Base | MEM + Earle’s Salts + NEAA |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 290.00–330.00 mOsm/kg H₂O |
| Total ingredients | 36 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | 5% CO₂ required (sodium bicarbonate buffering) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MEM with Earle’s Salts and NEAA: 36 ingredients verified per lot with CAS numbers. The AMINO ACIDS tab includes both essential amino acids (EAA) and Non-Essential Amino Acids as one combined group per the source composition table.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 126.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 52.000 |
| L-Leucine | 61-90-5 | 52.000 |
| L-Lysine hydrochloride | 657-27-2 | 72.500 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 32.000 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 48.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 51.900 |
| L-Valine | 72-18-4 | 46.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system, with particular care for primary cell–grade purity standards.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants that alter primary cell electrophysiology, ion channel activity, and TEER measurements.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL — below TLR4 activation threshold for primary cell cultures.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: 290.00–330.00 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-MEM-R1X compares
FluxMPS™ DCP-MEM-R1X vs. conventional 0.22 µm–filtered MEM and standard DMEM for primary cell OoC applications.
| Parameter | DCP-MEM-R1X (FluxMPS™) | Conventional MEM (0.22 µm filtered) |
Standard DMEM HG (0.22 µm filtered) |
|---|---|---|---|
| Full-formulation MEM with Earle's Salts and NEAA without Phenol Red — imaging-clean, hormone-interference–free primary cell base | check_circle Yes | cancel No | cancel No |
| Salt formulation | Earle’s Salts (5% CO₂ optimized) | Earle’s Salts | Modified Earle’s |
| NEAA included | check_circle Yes (Ala, Asn, Asp, Glu, Pro) | Optional add-on | cancel Not included |
| Glucose | 1.0 g/L (Low — physiological) | 1.0 g/L | 4.5 g/L (High) |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
Frequently asked questions
Common questions about FluxMPS™ DCP-MEM-R1X — MEM with Earle’s Salts and NEAA.
Supporting literature
Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
