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FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine: 1X Liquid

Product#: DCP-MEM-Q1X
$44.00
DCP-MEM-Q1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without L-Glutamine

FluxMPS™ DCP-MEM-Q1X is a Microfluidics Suitable, ultra-filtered Minimum Essential Medium (MEM) with Earle’s Salts and Non-Essential Amino Acids (NEAA) formulation engineered for primary cell culture on organ-on-a-chip (OoC), neuronal chip, and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it is finished to a 0.04 µm final cut-off for low-particulate perfusion in microfluidic channels. Formulation: [+] Earle’s Salts, [+] NEAA, [+] Low Glucose (1000 mg/L), [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] L-Glutamine.

  • MEM with Earle’s Salts — physiological ionic balance optimized for primary fibroblasts, neurons, epithelial, and vascular cells
  • NEAA (alanine, asparagine, aspartate, glutamate, proline) pre-loaded to reduce de novo synthesis burden on primary cells
  • Low Glucose (1.0 g/L) — physiological carbon source for primary cell types sensitive to high glucose-induced ROS and glycation
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Sodium bicarbonate–buffered (2200 mg/L NaHCO₃) for standard 5% CO₂ incubation
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom formulation modifications (pH, glucose, NEAA, HEPES) available on request
DCP-MEM-Q1X Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Available sizes: 500 mL, 1000 mL
Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine: 1X Liquid
  • BaseMEM + Earle’s Salts + NEAA
  • Glucose1000 mg/L (1.0 g/L, Low Glucose)
  • Formulation[+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290 – 330 mOsm/kg H¹O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
  • CO₂ Requirement5% CO₂ required (NaHCO₃-buffered)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for primary cell culture where standard media fails

MEM with Earle’s Salts is the primary cell biologist’s medium — but conventional 0.22 µm–filtered MEM passes mycoplasma-scale particulate and endotoxin fragments that can alter primary cell gene expression, activation state, and morphology. FluxMPS™ reaches a 0.04 µm final polish while preserving the full MEM + NEAA nutritional profile primary cells depend on.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance supports safe perfusion in neuronal, epithelial, and fibroblast chip architectures.

biotech

Primary cell–optimized formulation

MEM + Earle’s Salts + NEAA: the established base for primary fibroblasts, neurons, epithelial cells, and vascular smooth muscle cells in low-serum or serum-free conditions.

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Trace-metal & TOC-controlled water

Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm), minimizing trace-metal and organic-carbon background in the finished medium.

shield

Low endotoxin release specification

< 0.05 EU/mL endotoxin release specification, tested per batch by LAL assay — relevant to primary cell TLR4-mediated inflammatory sensitivity.

science

NEAA reduces metabolic burden

Pre-loaded NEAA reduces the de novo synthesis burden on primary cells, supporting viability in low-serum conditions.

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Customization on demand

pH, glucose, NEAA concentrations, HEPES, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reach a final 0.04 µm polish. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter, giving full redundancy across the train.

  1. 1

    0.1 µm Prefiltration I — Large Particulate Removal

    Removes large aggregates and contaminants; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I — Mycoplasma-Retentive Filtration

    Retains particulate at the mycoplasma size range (0.2–0.3 µm) — a class of retention absent from standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II — Second-Pass Redundancy

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in an ISO Class 5 (Class 100) environment.

Filtration architecture summary

FluxMPS™ DCP-MEM-Q1X is processed through two paired prefilter + final-filter stages (0.1 µm ×2 + 0.04 µm ×2), reaching a validated 0.04 µm final pore size — well below the 0.22 µm cut-off used in conventional MEM.

4
Sequential filtration passes
0.04
µm Final pore size
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm and 0.04 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-MEM-Q1X Minimum Essential Medium (MEM), Low Glucose, NEAA w/o L-Glutamine: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I (mycoplasma-retentive), 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable MEM Earle Salts NEAA for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) used in primary-cell OoC applications.
© Diagnocine® — DCP-MEM-Q1X
Applications

Primary cell models & OoC applications

FluxMPS™ DCP-MEM-Q1X is purpose-built for primary cell OoC platforms where low-glucose, NEAA-supplemented, Earle’s salt–balanced conditions better mimic the physiological microenvironment than DMEM — combined with 0.04 µm filtration purity for particle-free chip perfusion.

Automated Bioreactors & Robotics

Next-Generation Primary Cell Perfusion

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available as a separate product line for automated bioreactor perfusion where trace particulates accelerate chip fouling. This is a distinct grade from the 0.04 µm Microfluidics Suitable product described on this page.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from primary cell perfusion circuits
  • Valve & Sensor Protection: Reduces micro-fouling risk in delicate neuronal and epithelial chip geometries
  • Extended Perfusion Stability: Consistent NEAA and nutrient delivery over multi-week primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Neuroscience

Neuronal Chips & Brain-on-Chip

MEM + Earle’s Salts + NEAA + low glucose is a physiologically close base for primary cortical neurons, DRG neurons, and iPSC-derived neuronal networks in microfluidic compartmentalized chips.

Primary neuronsiPSC-NeuronsDRG neuronsBrain-on-chip
Epithelial Biology

Epithelium-on-Chip

Low-glucose MEM with NEAA supports primary epithelial cells (intestinal, pulmonary, renal) and barrier integrity in transwell and microfluidic TEER-monitored platforms.

Caco-2A549Primary HBECGut-on-chip
Connective Tissue

Primary Fibroblast & Stromal Models

MEM with NEAA is a classical culture base for primary dermal, lung, and cardiac fibroblasts — NEAA reduces glutamine-driven ammonia accumulation associated with myofibroblast differentiation artefacts.

Primary fibroblastsLung fibroblastsCardiac fibroblasts
Vascular Biology

Vascular Cell Culture

Earle’s salt ionic balance and low glucose support vascular smooth muscle cells (VSMCs) and primary endothelial co-culture models on vascular-on-chip platforms.

VSMCsPrimary endothelialVascular-on-chip
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered MEM reduces particulate load relevant to sub-100 µm neuronal and epithelial chip microchannels.

OoCToCLoCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate MEM supports confocal microscopy and biosensor applications. This formulation contains phenol red (11 mg/L); request the phenol-red-free variant where reduced optical background is required.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (1000 mg/L, Low), [+] Sodium Pyruvate | [-] L-Glutamine
Base MEM + Earle’s Salts + NEAA
Appearance Orange-to-red colored, clear solution (phenol red present)
Glucose 1000 mg/L (1.0 g/L, Low Glucose)
pH USP <791> 7.4
Osmolality USP <785> 290 – 330 mOsm/kg H¹O
Total ingredients 36 (4 category groups: Inorganic Salts, Amino Acids, Vitamins, Others; displayed across 3 tabs)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (per batch)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm + 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ required (2200 mg/L NaHCO₃-buffered)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MEM with Earle’s Salts and NEAA: 36 ingredients verified per lot with CAS numbers. The Amino Acids tab combines essential amino acids with the Non-Essential Amino Acids as one group, per the source composition table.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 126.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 31.300
L-Glutamic acid 56-86-0 14.700
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 52.000
L-Leucine 61-90-5 52.000
L-Lysine hydrochloride 657-27-2 72.500
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 32.000
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 48.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-15-0 51.900
L-Valine 72-18-4 46.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
Phenol red sodium salt 34487-61-1 11.000
Sodium pyruvate 113-24-6 110.000
Custom formulation: NEAA concentrations, HEPES addition, glucose level, and pH adjustments available on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA at the Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm, trace-metal and organic-carbon (TOC) controlled to minimize chemical background variability.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Release specification: 290 – 330 mOsm/kg H¹O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-MEM-Q1X compares

FluxMPS™ DCP-MEM-Q1X vs. conventional 0.22 µm–filtered MEM and standard high-glucose DMEM for primary cell OoC applications.

Parameter DCP-MEM-Q1X (FluxMPS™) Conventional MEM
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
MEM with Earle’s Salts and NEAA without L-Glutamine — fresh nitrogen addition for ammonia-sensitive primary cell models check_circle Yes cancel No cancel No
Salt formulation Earle’s Salts (5% CO₂ optimized) Earle’s Salts Modified Earle’s
NEAA included check_circle Yes (Ala, Asn, Asp, Glu, Pro) Optional add-on cancel Not included
Glucose 1.0 g/L (Low) 1.0 g/L 4.5 g/L (High)
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.04 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available on request cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as “Not specified”.

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-MEM-Q1X — MEM with Earle’s Salts and NEAA.

Yes. DCP-MEM-Q1X is processed through a Quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels for MPS, OoC, and LoC platforms. MEM with Earle’s Salts and NEAA is particularly suited to primary cell OoC models — neuronal chips, epithelial chips, fibroblast chips — where low glucose and NEAA supplementation better mimic the physiological microenvironment than DMEM.
FluxMPS™ uses four sequential filters — 0.1 µm Prefiltration I, 0.04 µm Final filtration I, 0.1 µm Prefiltration II, and 0.04 µm Final filtration II Polish — reaching a 0.04 µm final cut-off, well below the 0.22 µm used in conventional MEM. This paired-stage architecture gives full redundancy: each 0.04 µm final filter has its own dedicated 0.1 µm prefilter.
L-glutamine is omitted for fresh addition at time of use (commonly 2 mM / 292 mg/L), since L-glutamine degrades in solution over time to pyrrolidone carboxylic acid and ammonia — a particular concern in media intended for sensitive primary cells. The NEAA supplement remains pre-loaded: alanine, asparagine, aspartate, glutamate, and proline are already present in this formulation; only L-glutamine is added fresh. GlutaMAX dipeptide is an option for extended culture stability.
Yes. DCP-MEM-Q1X uses Earle’s Salts with sodium bicarbonate (2200 mg/L NaHCO₃) as the buffering system, which requires a standard 5% CO₂ incubator to maintain a stable pH near 7.4. This differs from Hanks’ Salts formulations, which omit bicarbonate and are designed for CO₂-independent environments.
Yes. Serum and other protein-containing supplements should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not appropriate for this purpose, as it will retain much of the protein and lipoprotein fraction of serum. Defined, protein-free additions can be filtered at 0.1 µm.
The release specification is < 0.05 EU/mL, verified per manufacturing batch by LAL assay (USP <85>), with an assay sensitivity of 0.005 EU/mL. Endotoxin is controlled at the batch level rather than per individual unit; every batch is tested before release and must meet this specification.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MEM with Earle’s Salts and NEAA for primary cell culture and organ-on-a-chip applications.

  1. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130:432–437. doi:10.1126/science.130.3373.432
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  6. Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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